{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:34:18Z","timestamp":1767897258912,"version":"3.49.0"},"reference-count":58,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,3,10]],"date-time":"2020-03-10T00:00:00Z","timestamp":1583798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["BFU2015-65623"],"award-info":[{"award-number":["BFU2015-65623"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PGC2018-098557-B-I00"],"award-info":[{"award-number":["PGC2018-098557-B-I00"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020","doi-asserted-by":"publisher","award":["grant agreement No 765912"],"award-info":[{"award-number":["grant agreement No 765912"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["6619\/1-1"],"award-info":[{"award-number":["6619\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing mitophagy in vivo. We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito-QC mice to quantify mitophagy activation in vitro and ex vivo. We also analyzed mitophagy in retinal ganglion cells (RGCs), in vivo, using different mitophagy inducers, as well as after optic nerve crush (ONC) in mice, a commonly used surgical procedure to model glaucoma. Using mito-QC reporter we quantified mitophagy induced by several known inducers in primary RGCs in vitro, ex vivo and in vivo. We also found that RGCs were rescued from some glaucoma relevant stress factors by incubation with the iron chelator deferiprone (DFP). Thus, the mito-QC reporter-based model is a valuable tool for accurately analyzing mitophagy in the context of glaucoma.<\/jats:p>","DOI":"10.3390\/ijms21051882","type":"journal-article","created":{"date-parts":[[2020,3,10]],"date-time":"2020-03-10T11:59:36Z","timestamp":1583841576000},"page":"1882","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models"],"prefix":"10.3390","volume":"21","author":[{"given":"Ines","family":"Rosignol","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Biology, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, CSIC, 28040 Madrid, Spain"}]},{"given":"Beatriz","family":"Villarejo-Zori","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, CSIC, 28040 Madrid, Spain"}]},{"given":"Petra","family":"Teresak","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, CSIC, 28040 Madrid, Spain"}]},{"given":"Elena","family":"Sierra-Filardi","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, CSIC, 28040 Madrid, Spain"}]},{"given":"Xandra","family":"Pereiro","sequence":"additional","affiliation":[{"name":"Experimental-Ophthalmo-Biology Group, Universidad del Pa\u00eds Vasco, 48950 Leioa, Spain"}]},{"given":"Natalia","family":"Rodr\u00edguez-Muela","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, CSIC, 28040 Madrid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1672-5132","authenticated-orcid":false,"given":"Elena","family":"Vecino","sequence":"additional","affiliation":[{"name":"Experimental-Ophthalmo-Biology Group, Universidad del Pa\u00eds Vasco, 48950 Leioa, Spain"}]},{"given":"Helena L. A.","family":"Vieira","sequence":"additional","affiliation":[{"name":"UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"CEDOC, NOVA Medical School\/Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Nova de Lisboa, 1150-082 Lisbon, Portugal"}]},{"given":"Katharina","family":"Bell","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, CSIC, 28040 Madrid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3045-951X","authenticated-orcid":false,"given":"Patricia","family":"Boya","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Centro de Investigaciones Biol\u00f3gicas Margarita Salas, CSIC, 28040 Madrid, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1136\/bjo.86.2.238","article-title":"The definition and classification of glaucoma in prevalence surveys","volume":"86","author":"Foster","year":"2002","journal-title":"Br. 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