{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:50:11Z","timestamp":1760237411422,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T00:00:00Z","timestamp":1586390400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/FIS\/04564\/2016","POCI-01-0145-FEDER-031743 \u2013PTDC\/BIA-CEL\/31743\/2017"],"award-info":[{"award-number":["UID\/FIS\/04564\/2016","POCI-01-0145-FEDER-031743 \u2013PTDC\/BIA-CEL\/31743\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["CENTRO-01-0145-FEDER-000014"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000014"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"publisher","award":["P1-0055","P1-0390"],"award-info":[{"award-number":["P1-0055","P1-0390"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"name":"EraCoSysMed","award":["JTC-2 2017"],"award-info":[{"award-number":["JTC-2 2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Keratins are one of the most abundant proteins in epithelial cells. They form a cytoskeletal filament network whose structural organization seriously conditions its function. Dynamic keratin particles and aggregates are often observed at the periphery of mutant keratinocytes related to the hereditary skin disorder epidermolysis bullosa simplex, which is due to mutations in keratins 5 and 14. To account for their emergence in mutant cells, we extended an existing mathematical model of keratin turnover in wild-type cells and developed a novel 2D phase-field model to predict the keratin distribution inside the cell. This model includes the turnover between soluble, particulate and filamentous keratin forms. We assumed that the mutation causes a slowdown in the assembly of an intermediate keratin phase into filaments, and demonstrated that this change is enough to account for the loss of keratin filaments in the cell\u2019s interior and the emergence of keratin particles at its periphery. The developed mathematical model is also particularly tailored to model the spatial distribution of keratins as the cell changes its shape.<\/jats:p>","DOI":"10.3390\/ijms21072596","type":"journal-article","created":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T03:40:19Z","timestamp":1586403619000},"page":"2596","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Keratin Dynamics and Spatial Distribution in Wild-Type and K14 R125P Mutant Cells\u2014A Computational Model"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2552-9993","authenticated-orcid":false,"given":"Marcos","family":"Gouveia","sequence":"first","affiliation":[{"name":"CFisUC, Center for Physics of the University of Coimbra, Department of Physics, University of Coimbra, R Larga, 3004-516 Coimbra, Portugal"}]},{"given":"\u0160pela","family":"Zemlji\u010d-Jokhadar","sequence":"additional","affiliation":[{"name":"Institute for Biophysics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia"}]},{"given":"Marko","family":"Vidak","sequence":"additional","affiliation":[{"name":"Medical Center for Molecular Biology, Institute for Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia"}]},{"given":"Biljana","family":"Stojkovi\u010d","sequence":"additional","affiliation":[{"name":"Institute for Biophysics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia"}]},{"given":"Jure","family":"Derganc","sequence":"additional","affiliation":[{"name":"Institute for Biophysics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6078-0721","authenticated-orcid":false,"given":"Rui","family":"Travasso","sequence":"additional","affiliation":[{"name":"CFisUC, Center for Physics of the University of Coimbra, Department of Physics, University of Coimbra, R Larga, 3004-516 Coimbra, Portugal"}]},{"given":"Mirjana","family":"Liovic","sequence":"additional","affiliation":[{"name":"Medical Center for Molecular Biology, Institute for Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"18513","DOI":"10.1073\/pnas.1313491110","article-title":"Keratins as the main component for the mechanical integrity of keratinocytes","volume":"110","author":"Ramms","year":"2013","journal-title":"Proc. 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