{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T07:02:30Z","timestamp":1778050950778,"version":"3.51.4"},"reference-count":39,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T00:00:00Z","timestamp":1599955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["DL 57\/2016\/CP1382\/CT0012, and SFRH\/BPD\/89427\/2012, UID\/AGR\/04129\/2020 (LEAF) and UID\/DTP\/04138 \/2019 (i.Med)"],"award-info":[{"award-number":["DL 57\/2016\/CP1382\/CT0012, and SFRH\/BPD\/89427\/2012, UID\/AGR\/04129\/2020 (LEAF) and UID\/DTP\/04138 \/2019 (i.Med)"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Nodulin 26-like intrinsic proteins (NIPs) of the plant aquaporin family majorly facilitate the transport of physiologically relevant solutes. The present study intended to investigate how substrate selectivity in grapevine NIPs is influenced by the aromatic\/arginine (ar\/R) selectivity filter within the pore and the possible underlying mechanisms. A mutational approach was used to interchange the ar\/R residues between grapevine NIPs (VvTnNIP1;1 with VvTnNIP6;1, and VvTnNIP2;1 with VvTnNIP5;1). Their functional characterization by stopped-flow spectroscopy in Saccharomyces cerevisiae revealed that mutations in residues of H2\/H5 helices in VvTnNIP1;1 and VvTnNIP6;1 caused a general decline in membrane glycerol permeability but did not impart the expected substrate conductivity in the mutants. This result suggests that ar\/R filter substitution could alter the NIP channel activity, but it was not sufficient to interchange their substrate preferences. Further, homology modeling analyses evidenced that variations in the pore radius combined with the differences in the channel\u2019s physicochemical properties (hydrophilicity\/hydrophobicity) may drive substrate selectivity. Furthermore, yeast growth assays showed that H5 residue substitution alleviated the sensitivity of VvTnNIP2;1 and VvTnNIP5;1 to As, B, and Se, implying importance of H5 sequence for substrate selection. These results contribute to the knowledge of the overall determinants of substrate selectivity in NIPs.<\/jats:p>","DOI":"10.3390\/ijms21186697","type":"journal-article","created":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T21:11:32Z","timestamp":1600031492000},"page":"6697","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Insights into the Selectivity Mechanisms of Grapevine NIP Aquaporins"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0181-989X","authenticated-orcid":false,"given":"Farzana","family":"Sabir","sequence":"first","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food (LEAF), Departmento de Recursos Biol\u00f3gicos, Ambiente e Territ\u00f3rio (DRAT), Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"},{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8520-5165","authenticated-orcid":false,"given":"Antonella","family":"Di Pizio","sequence":"additional","affiliation":[{"name":"Leibniz-Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany"}]},{"given":"Maria C.","family":"Loureiro-Dias","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food (LEAF), Departmento de Recursos Biol\u00f3gicos, Ambiente e Territ\u00f3rio (DRAT), Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1599-9542","authenticated-orcid":false,"given":"Angela","family":"Casini","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Technical University of Munich, Lichtenbergstr. 4, 85748 M\u00fcnchen, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8487-110X","authenticated-orcid":false,"given":"Gra\u00e7a","family":"Soveral","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9307-1905","authenticated-orcid":false,"given":"Catarina","family":"Prista","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food (LEAF), Departmento de Recursos Biol\u00f3gicos, Ambiente e Territ\u00f3rio (DRAT), Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1146\/annurev.arplant.59.032607.092734","article-title":"Plant aquaporins: Membrane channels with multiple integrated functions","volume":"59","author":"Maurel","year":"2008","journal-title":"Annu. 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