{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T19:14:46Z","timestamp":1775070886872,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT - Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["IF\/00256\/2015"],"award-info":[{"award-number":["IF\/00256\/2015"]}]},{"name":"Fundo Europeu de Desenvolvimento Regional (FEDER) funds through the COMPETE 2020 Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia\/Minist\u00e9rio da","award":["POCI-01-0145-FEDER-029540 (PTDC\/BIA-OUT\/29540\/2017)"],"award-info":[{"award-number":["POCI-01-0145-FEDER-029540 (PTDC\/BIA-OUT\/29540\/2017)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cyanobacteria play an important role in several ecological environments, and they are widely accepted to be the ancestors of chloroplasts in modern plants and green algae. Cyanobacteria have become attractive models for metabolic engineering, with the goal of exploring them as microbial cell factories. However, the study of cyanobacterial lipids\u2019 composition and variation, and the assessment of the lipids\u2019 functional and structural roles have been largely overlooked. Here, we aimed at expanding the cyanobacterial lipidomic analytical pipeline by using an untargeted lipidomics approach. Thus, the lipid composition variation of the model cyanobacterium Synechocystis sp. PCC 6803 was investigated in response to both alternative cultivation setups and gene deletion. This approach allowed for detecting differences in total lipid content, alterations in fatty-acid unsaturation level, and adjustments of specific lipid species among the identified lipid classes. The employed method also revealed that the cultivation setup tested in this work induced a deeper alteration of the cyanobacterial cell lipidome than the deletion of a gene that results in a dramatic increase in the release of lipid-rich outer membrane vesicles. This study further highlights how growth conditions must be carefully selected when cyanobacteria are to be engineered and\/or scaled-up for lipid or fatty acids production.<\/jats:p>","DOI":"10.3390\/ijms21238883","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T02:34:42Z","timestamp":1606185282000},"page":"8883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Untargeted Lipidomics Analysis of the Cyanobacterium Synechocystis sp. PCC 6803: Lipid Composition Variation in Response to Alternative Cultivation Setups and to Gene Deletion"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1990-2130","authenticated-orcid":false,"given":"Weronika","family":"Hewelt-Belka","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemistry, Gda\u0144sk University of Technology, Gabriela Narutowicza 11\/12, 80-233 Gda\u0144sk, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2546-3779","authenticated-orcid":false,"given":"\u00c1gata","family":"Kot-Wasik","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemistry, Gda\u0144sk University of Technology, Gabriela Narutowicza 11\/12, 80-233 Gda\u0144sk, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4396-2122","authenticated-orcid":false,"given":"Paula","family":"Tamagnini","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal"},{"name":"IBMC\u2014Instituto de Biologia Molecular e Celular, Universidade do Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal"},{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, R. Campo Alegre s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-152X","authenticated-orcid":false,"given":"Paulo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal"},{"name":"IBMC\u2014Instituto de Biologia Molecular e Celular, Universidade do Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,24]]},"reference":[{"key":"ref_1","first-page":"36","article-title":"Synechocystis: Not Just a Plug-Bug for CO2, but a Green E. coli","volume":"2","author":"Du","year":"2014","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4515","DOI":"10.1038\/s41598-020-61100-4","article-title":"Synechocystis sp. PCC 6803 overexpressing genes involved in CBB cycle and free fatty acid cycling enhances the significant levels of intracellular lipids and secreted free fatty acids","volume":"10","author":"Eungrasamee","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6899","DOI":"10.1073\/pnas.1103014108","article-title":"Fatty acid production in genetically modified cyanobacteria","volume":"108","author":"Liu","year":"2011","journal-title":"Proc. Natl. Acad. Sci. 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