{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:22:45Z","timestamp":1774462965206,"version":"3.50.1"},"reference-count":86,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T00:00:00Z","timestamp":1606694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia \u2013 Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior (FCT-MCTES, Portugal)","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia \u2013 Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior (FCT-MCTES, Portugal)","award":["SPRH\/BD\/128290\/2017 and SPRH\/BD\/100517\/2014"],"award-info":[{"award-number":["SPRH\/BD\/128290\/2017 and SPRH\/BD\/100517\/2014"]}]},{"name":"Marie Sk\u0142odowska-Curie Research and Innovation Staff Exchange (MSCA-RISE, European Union) project INPACT","award":["grant agreement 644167"],"award-info":[{"award-number":["grant agreement 644167"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq, Brazil), Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES, Brazil), Funda\u00e7\u00e3o de Amparo a Pesquisa do Distrito Federal (FAPDF, Brazil)","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino, Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT, Brazil)","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Discovering antibiotic molecules able to hold the growing spread of antimicrobial resistance is one of the most urgent endeavors that public health must tackle. The case of Gram-negative bacterial pathogens is of special concern, as they are intrinsically resistant to many antibiotics, due to an outer membrane that constitutes an effective permeability barrier. Antimicrobial peptides (AMPs) have been pointed out as potential alternatives to conventional antibiotics, as their main mechanism of action is membrane disruption, arguably less prone to elicit resistance in pathogens. Here, we investigate the in vitro activity and selectivity of EcDBS1R4, a bioinspired AMP. To this purpose, we have used bacterial cells and model membrane systems mimicking both the inner and the outer membranes of Escherichia coli, and a variety of optical spectroscopic methodologies. EcDBS1R4 is effective against the Gram-negative E. coli, ineffective against the Gram-positive Staphylococcus aureus and noncytotoxic for human cells. EcDBS1R4 does not form stable pores in E. coli, as the peptide does not dissipate its membrane potential, suggesting an unusual mechanism of action. Interestingly, EcDBS1R4 promotes a hemi-fusion of vesicles mimicking the inner membrane of E. coli. This fusogenic ability of EcDBS1R4 requires the presence of phospholipids with a negative curvature and a negative charge. This finding suggests that EcDBS1R4 promotes a large lipid spatial reorganization able to reshape membrane curvature, with interesting biological implications herein discussed.<\/jats:p>","DOI":"10.3390\/ijms21239104","type":"journal-article","created":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T10:26:12Z","timestamp":1606731972000},"page":"9104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["EcDBS1R4, an Antimicrobial Peptide Effective against Escherichia coli with In Vitro Fusogenic Ability"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6929-6143","authenticated-orcid":false,"given":"Marcin","family":"Makowski","sequence":"first","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9398-2140","authenticated-orcid":false,"given":"M\u00e1rio R.","family":"Fel\u00edcio","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal"}]},{"given":"Isabel C. M.","family":"Fensterseifer","sequence":"additional","affiliation":[{"name":"Centro de An\u00e1lises Prote\u00f4micas e Bioqu\u00edmicas, P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Gen\u00f4micas e Biotecnologia, Universidade Cat\u00f3lica de Bras\u00edlia, Bras\u00edlia 71966-700, Brazil"},{"name":"S-Inova Biotech, P\u00f3s-gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-010, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9546-0525","authenticated-orcid":false,"given":"Oct\u00e1vio L.","family":"Franco","sequence":"additional","affiliation":[{"name":"Centro de An\u00e1lises Prote\u00f4micas e Bioqu\u00edmicas, P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Gen\u00f4micas e Biotecnologia, Universidade Cat\u00f3lica de Bras\u00edlia, Bras\u00edlia 71966-700, Brazil"},{"name":"S-Inova Biotech, P\u00f3s-gradua\u00e7\u00e3o em Biotecnologia, Universidade Cat\u00f3lica Dom Bosco, Campo Grande 79117-010, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0580-0475","authenticated-orcid":false,"given":"Nuno C.","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2903-4282","authenticated-orcid":false,"given":"S\u00f3nia","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,30]]},"reference":[{"key":"ref_1","unstructured":"(2020, March 17). 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