{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T05:45:32Z","timestamp":1768283132765,"version":"3.49.0"},"reference-count":79,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T00:00:00Z","timestamp":1614211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["FK125035"],"award-info":[{"award-number":["FK125035"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"name":"New National Excelence Program of the Ministry of Human Capacities","award":["\u00daNKP-18-4-DE-70"],"award-info":[{"award-number":["\u00daNKP-18-4-DE-70"]}]},{"name":"New National Excelence Program of the Ministry of Human Capacities","award":["\u00daNKP-19-4-DE-3"],"award-info":[{"award-number":["\u00daNKP-19-4-DE-3"]}]},{"name":"New National Excelence Program of the Ministry of Human Capacities","award":["\u00daNKP-17-3\/I"],"award-info":[{"award-number":["\u00daNKP-17-3\/I"]}]},{"name":"Hungarian Brain Research Program","award":["KTIA_NAP_13-2-2014-0005"],"award-info":[{"award-number":["KTIA_NAP_13-2-2014-0005"]}]},{"name":"Hungarian Brain Reseach Program","award":["2017-1.2.1-NKP-2017-00002"],"award-info":[{"award-number":["2017-1.2.1-NKP-2017-00002"]}]},{"name":"J\u00e1nos Bolyai Scholarship of the Hungarian Academy of Sciences","award":["BO\/00369\/17\/5"],"award-info":[{"award-number":["BO\/00369\/17\/5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The phosphorylation of serine 10 in histone 3 (p-S10H3) has recently been demonstrated to participate in spinal nociceptive processing. However, superficial dorsal horn (SDH) neurons involved in p-S10H3-mediated nociception have not been fully characterized. In the present work, we combined immunohistochemistry, in situ hybridization with the retrograde labeling of projection neurons to reveal the subset of dorsal horn neurons presenting an elevated level of p-S10H3 in response to noxious heat (60 \u00b0C), causing burn injury. Projection neurons only represented a small percentage (5%) of p-S10H3-positive cells, while the greater part of them belonged to excitatory SDH interneurons. The combined immunolabeling of p-S10H3 with markers of already established interneuronal classes of the SDH revealed that the largest subset of neurons with burn injury-induced p-S10H3 expression was dynorphin immunopositive in mice. Furthermore, the majority of p-S10H3-expressing dynorphinergic neurons proved to be excitatory, as they lacked Pax-2 and showed Lmx1b-immunopositivity. Thus, we showed that neurochemically heterogeneous SDH neurons exhibit the upregulation of p-S10H3 shortly after noxious heat-induced burn injury and consequential tissue damage, and that a dedicated subset of excitatory dynorphinergic neurons is likely a key player in the development of central sensitization via the p-S10H3 mediated pathway.<\/jats:p>","DOI":"10.3390\/ijms22052297","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T02:08:17Z","timestamp":1614305297000},"page":"2297","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Spinal Excitatory Dynorphinergic Interneurons Contribute to Burn Injury-Induced Nociception Mediated by Phosphorylated Histone 3 at Serine 10 in Rodents"],"prefix":"10.3390","volume":"22","author":[{"given":"Angelika","family":"Varga","sequence":"first","affiliation":[{"name":"MTA-DE-NAP B-Pain Control Research Group, University of Debrecen, 4032 Debrecen, Hungary"},{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary"}]},{"given":"Zolt\u00e1n","family":"M\u00e9sz\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary"}]},{"given":"Mikl\u00f3s","family":"Sivad\u00f3","sequence":"additional","affiliation":[{"name":"MTA-DE-NAP B-Pain Control Research Group, University of Debrecen, 4032 Debrecen, Hungary"},{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary"}]},{"given":"T\u00edmea","family":"B\u00e1cskai","sequence":"additional","affiliation":[{"name":"Division of Dental Anatomy, Department of Basic Medical Sciences, Faculty of Dentistry, University of Debrecen, 4032 Debrecen, Hungary"}]},{"given":"Bence","family":"V\u00e9gh","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary"}]},{"given":"\u00c9va","family":"K\u00f3kai","sequence":"additional","affiliation":[{"name":"MTA-DE-NAP B-Pain Control Research Group, University of Debrecen, 4032 Debrecen, Hungary"},{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary"}]},{"given":"Istv\u00e1n","family":"Nagy","sequence":"additional","affiliation":[{"name":"Department of Surgery and Cancer, Imperial College London, London SW7 ZAZ, UK"},{"name":"Department of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary"}]},{"given":"P\u00e9ter","family":"Sz\u00fccs","sequence":"additional","affiliation":[{"name":"MTA-DE-NAP B-Pain Control Research Group, University of Debrecen, 4032 Debrecen, Hungary"},{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1744806916641950","DOI":"10.1177\/1744806916641950","article-title":"Epigenetic regulation of spinal cord gene expression contributes to enhanced postoperative pain and analgesic tolerance subsequent to continuous opioid exposure","volume":"12","author":"Sahbaie","year":"2016","journal-title":"Mol. 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