{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T04:47:03Z","timestamp":1764910023849,"version":"build-2065373602"},"reference-count":75,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["UIDB 50006\/2020"],"award-info":[{"award-number":["UIDB 50006\/2020"]}]},{"name":"GEDII - Grupo de Estudos da Doen\u00e7a Inflamat\u00f3ria Intestinal","award":["-"],"award-info":[{"award-number":["-"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Angiotensin II (Ang II) regulates colon contraction, acting not only directly on smooth muscle but also indirectly, interfering with myenteric neuromodulation mediated by the activation of AT1 \/AT2 receptors. In this article, we aimed to explore which mediators and cells were involved in Ang II-mediated colonic contraction in the TNBS-induced rat model of colitis. The contractile responses to Ang II were evaluated in distinct regions of the colon of control animals or animals with colitis in the absence and presence of different antagonists\/inhibitors. Endogenous levels of Ang II in the colon were assessed by ELISA and the number of AT1\/AT2 receptors by qPCR. Ang II caused AT1 receptor-mediated colonic contraction that was markedly decreased along the colons of TNBS-induced rats, consistent with reduced AT1 mRNA expression. However, the effect mediated by Ang II is much more intricate, involving (in addition to smooth muscle cells and nerve terminals) ICC and EGC, which communicate by releasing ACh and NO in a complex mechanism that changes colitis, unveiling new therapeutic targets.<\/jats:p>","DOI":"10.3390\/ijms22094836","type":"journal-article","created":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T11:06:01Z","timestamp":1620212761000},"page":"4836","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Interaction between the Renin\u2013Angiotensin System and Enteric Neurotransmission Contributes to Colonic Dysmotility in the TNBS-Induced Model of Colitis"],"prefix":"10.3390","volume":"22","author":[{"given":"Mariana","family":"Ferreira-Duarte","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"}]},{"given":"Tiago","family":"Rodrigues-Pinto","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"}]},{"given":"Teresa","family":"Sousa","sequence":"additional","affiliation":[{"name":"Unit of Pharmacology and Therapeutics, Department of Biomedicine, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal"},{"name":"Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar, University of Porto (ICBAS-UP), 4050-313 Porto, Portugal"}]},{"given":"Miguel A.","family":"Faria","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"}]},{"given":"Maria Sofia","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"}]},{"given":"Daniela","family":"Menezes-Pinto","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"}]},{"given":"Marisa","family":"Esteves-Monteiro","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2634-9668","authenticated-orcid":false,"given":"Fernando","family":"Magro","sequence":"additional","affiliation":[{"name":"Unit of Pharmacology and Therapeutics, Department of Biomedicine, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal"}]},{"given":"Patr\u00edcia","family":"Dias-Pereira","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar, University of Porto (ICBAS-UP), 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4727-727X","authenticated-orcid":false,"given":"Margarida","family":"Duarte-Ara\u00fajo","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"},{"name":"Department of Immuno-Physiology and Pharmacology, Institute of Biomedical Sciences Abel Salazar, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9509-0613","authenticated-orcid":false,"given":"Manuela","family":"Morato","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Pharmacy of University of Porto (FFUP), 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1016\/S0140-6736(17)32448-0","article-title":"Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: A systematic review of population-based studies","volume":"390","author":"Ng","year":"2017","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.3748\/wjg.v24.i22.2363","article-title":"Quality of care in inflammatory bowel diseases: What is the best way to better outcomes?","volume":"24","author":"Strohl","year":"2018","journal-title":"World J. 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