{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T13:54:12Z","timestamp":1773496452307,"version":"3.50.1"},"reference-count":78,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["ED431B 2019\/02"],"award-info":[{"award-number":["ED431B 2019\/02"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Operational Program for Competitiveness and Internationalisation through European Regional Development Funds (FEDER\/FNR) and through national funds by the Portuguese Foundation for Science and Technology","award":["PTDC\/MED-TOX\/31162\/2017"],"award-info":[{"award-number":["PTDC\/MED-TOX\/31162\/2017"]}]},{"name":"COMPETE 2020, Portugal 2020 and European Union, through FEDER","award":["PTDC\/SAU-PUB\/29651\/2017"],"award-info":[{"award-number":["PTDC\/SAU-PUB\/29651\/2017"]}]},{"DOI":"10.13039\/501100003176","name":"Ministerio de Educaci\u00f3n, Cultura y Deporte","doi-asserted-by":"publisher","award":["BEAGAL18\/00142"],"award-info":[{"award-number":["BEAGAL18\/00142"]}],"id":[{"id":"10.13039\/501100003176","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/101060\/2014"],"award-info":[{"award-number":["SFRH\/BD\/101060\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Standard toxicity tests might not be fully adequate for evaluating nanomaterials since their unique features are also responsible for unexpected interactions. The in vitro cytokinesis-block micronucleus (CBMN) test is recommended for genotoxicity testing, but cytochalasin-B (Cyt-B) may interfere with nanoparticles (NP), leading to inaccurate results. Our objective was to determine whether Cyt-B could interfere with MN induction by TiO2 NP in human SH-SY5Y cells, as assessed by CBMN test. Cells were treated for 6 or 24 h, according to three treatment options: co-treatment with Cyt-B, post-treatment, and delayed co-treatment. Influence of Cyt-B on TiO2 NP cellular uptake and MN induction as evaluated by flow cytometry (FCMN) were also assessed. TiO2 NP were significantly internalized by cells, both in the absence and presence of Cyt-B, indicating that this chemical does not interfere with NP uptake. Dose-dependent increases in MN rates were observed in CBMN test after co-treatment. However, FCMN assay only showed a positive response when Cyt-B was added simultaneously with TiO2 NP, suggesting that Cyt-B might alter CBMN assay results. No differences were observed in the comparisons between the treatment options assessed, suggesting they are not adequate alternatives to avoid Cyt-B interference in the specific conditions tested.<\/jats:p>","DOI":"10.3390\/ijms22168558","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T09:03:53Z","timestamp":1628499833000},"page":"8558","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells"],"prefix":"10.3390","volume":"22","author":[{"given":"Natalia","family":"Fern\u00e1ndez-Bert\u00f3lez","sequence":"first","affiliation":[{"name":"Universidade da Coru\u00f1a, Grupo DICOMOSA, Centro de Investigaciones Cient\u00edficas Avanzadas (CICA), Departamento de Psicolog\u00eda, Facultad de Ciencias de la Educaci\u00f3n, Campus Elvi\u00f1a s\/n, 15071 A Coru\u00f1a, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), AE CICA-INIBIC. Oza, 15071 A Coru\u00f1a, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2933-8561","authenticated-orcid":false,"given":"F\u00e1tima","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"EPIUnit\u2014Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, Rua das Taipas, N\u00b0 135, 4050-600 Porto, Portugal"},{"name":"Environmental Health Department, Portuguese National Institute of Health, Rua Alexandre Herculano 321, 4000-055 Porto, Portugal"},{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"Laboratory for Integrative and Translational Research in Population Health (ITR), Rua das Taipas 135, 4050-600 Porto, Portugal"}]},{"given":"Carla","family":"Costa","sequence":"additional","affiliation":[{"name":"EPIUnit\u2014Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, Rua das Taipas, N\u00b0 135, 4050-600 Porto, Portugal"},{"name":"Environmental Health Department, Portuguese National Institute of Health, Rua Alexandre Herculano 321, 4000-055 Porto, Portugal"},{"name":"Laboratory for Integrative and Translational Research in Population Health (ITR), Rua das Taipas 135, 4050-600 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0650-8852","authenticated-orcid":false,"given":"Eduardo","family":"P\u00e1saro","sequence":"additional","affiliation":[{"name":"Universidade da Coru\u00f1a, Grupo DICOMOSA, Centro de Investigaciones Cient\u00edficas Avanzadas (CICA), Departamento de Psicolog\u00eda, Facultad de Ciencias de la Educaci\u00f3n, Campus Elvi\u00f1a s\/n, 15071 A Coru\u00f1a, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), AE CICA-INIBIC. Oza, 15071 A Coru\u00f1a, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8693-5250","authenticated-orcid":false,"given":"Jo\u00e3o Paulo","family":"Teixeira","sequence":"additional","affiliation":[{"name":"EPIUnit\u2014Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, Rua das Taipas, N\u00b0 135, 4050-600 Porto, Portugal"},{"name":"Environmental Health Department, Portuguese National Institute of Health, Rua Alexandre Herculano 321, 4000-055 Porto, Portugal"},{"name":"Laboratory for Integrative and Translational Research in Population Health (ITR), Rua das Taipas 135, 4050-600 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7649-2599","authenticated-orcid":false,"given":"Blanca","family":"Laffon","sequence":"additional","affiliation":[{"name":"Universidade da Coru\u00f1a, Grupo DICOMOSA, Centro de Investigaciones Cient\u00edficas Avanzadas (CICA), Departamento de Psicolog\u00eda, Facultad de Ciencias de la Educaci\u00f3n, Campus Elvi\u00f1a s\/n, 15071 A Coru\u00f1a, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), AE CICA-INIBIC. Oza, 15071 A Coru\u00f1a, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5572-1089","authenticated-orcid":false,"given":"Vanessa","family":"Valdiglesias","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), AE CICA-INIBIC. Oza, 15071 A Coru\u00f1a, Spain"},{"name":"Universidade da Coru\u00f1a, Grupo DICOMOSA, Centro de Investigaciones Cient\u00edficas Avanzadas (CICA), Departamento de Biolog\u00eda, Facultad de Ciencias, Campus A Zapateira s\/n, 15071, 15071 A Coru\u00f1a, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1080\/10408444.2017.1328400","article-title":"A comprehensive framework for evaluating the environmental health and safety implications of engineered nanomaterials","volume":"47","author":"Boyes","year":"2017","journal-title":"Crit. Rev. 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