{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T09:45:24Z","timestamp":1775382324569,"version":"3.50.1"},"reference-count":59,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIAFBT\/32013\/2017"],"award-info":[{"award-number":["PTDC\/BIAFBT\/32013\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/05748\/2020"],"award-info":[{"award-number":["UIDB\/05748\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/05748\/2020"],"award-info":[{"award-number":["UIDP\/05748\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Adverse conditions caused by abiotic stress modulate plant development and growth by altering morphological and cellular mechanisms. Plants\u2019 responses\/adaptations to stress often involve changes in the distribution and sorting of specific proteins and molecules. Still, little attention has been given to the molecular mechanisms controlling these rearrangements. We tested the hypothesis that plants respond to stress by remodelling their endomembranes and adapting their trafficking pathways. We focused on the molecular machinery behind organelle biogenesis and protein trafficking under abiotic stress conditions, evaluating their effects at the subcellular level, by looking at ultrastructural changes and measuring the expression levels of genes involved in well-known intracellular routes. The results point to a differential response of the endomembrane system, showing that the genes involved in the pathway to the Protein Storage Vacuole and the exocyst-mediated routes are upregulated. In contrast, the ones involved in the route to the Lytic Vacuole are downregulated. These changes are accompanied by morphological alterations of endomembrane compartments. The data obtained demonstrate that plants\u2019 response to abiotic stress involves the differential expression of genes related to protein trafficking machinery, which can be connected to the activation\/deactivation of specific intracellular sorting pathways and lead to alterations in the cell ultrastructure.<\/jats:p>","DOI":"10.3390\/ijms221910644","type":"journal-article","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T10:55:40Z","timestamp":1633085740000},"page":"10644","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Abiotic Stress Triggers the Expression of Genes Involved in Protein Storage Vacuole and Exocyst-Mediated Routes"],"prefix":"10.3390","volume":"22","author":[{"given":"Jo\u00e3o","family":"Neves","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4917-9027","authenticated-orcid":false,"given":"Miguel","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"}]},{"given":"Ana","family":"S\u00e9neca","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7938-1074","authenticated-orcid":false,"given":"Susana","family":"Pereira","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9489-9904","authenticated-orcid":false,"given":"Jos\u00e9","family":"Pissarra","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8050-5102","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Pereira","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, s\/n\u00b0, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/pce.13203","article-title":"Adapting legume crops to climate change using genomic approaches","volume":"42","author":"Bayer","year":"2019","journal-title":"Plant Cell Environ."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1093\/jxb\/erx395","article-title":"All roads lead to the vacuole\u2014autophagic transport as part of the endomembrane trafficking network in plants","volume":"69","author":"Kalinowska","year":"2018","journal-title":"J. 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