{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T07:25:29Z","timestamp":1775201129340,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T00:00:00Z","timestamp":1634515200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In recent decades, atmospheric pollution led to a progressive reduction of the ozone layer with a consequent increase in UV-B radiation. Despite the high adaptation of olive trees to the Mediterranean environment, the progressive increase of UV-B radiation is a risk factor for olive tree cultivation. It is therefore necessary to understand how high levels of UV-B radiation affect olive plants and to identify olive varieties which are better adapted. In this study we analyzed two Italian olive varieties subjected to chronic UV-B stress. We focused on the effects of UV-B radiation on RubisCO, in terms of quantity, enzymatic activity and isoform composition. In addition, we also analyzed changes in the activity of antioxidant enzymes (SOD, CAT, GPox) to get a comprehensive picture of the antioxidant system. We also evaluated the effects of UV-B on the enzyme sucrose synthase. The overall damage at biochemical level was also assessed by analyzing changes in Hsp70, a protein triggered under stress conditions. The results of this work indicate that the varieties (Giarraffa and Olivastra Seggianese) differ significantly in the use of specific antioxidant defense systems, as well as in the activity and isoform composition of RubisCO. Combined with a different use of sucrose synthase, the overall picture shows that Giarraffa optimized the use of GPox and opted for a targeted choice of RubisCO isoforms, in addition to managing the content of sucrose synthase, thereby saving energy during critical stress points.<\/jats:p>","DOI":"10.3390\/ijms222011214","type":"journal-article","created":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T22:52:47Z","timestamp":1634683967000},"page":"11214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Olive Varieties under UV-B Stress Show Distinct Responses in Terms of Antioxidant Machinery and Isoform\/Activity of RubisCO"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8755-912X","authenticated-orcid":false,"given":"Chiara","family":"Piccini","sequence":"first","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"},{"name":"Institute for BioEconomy, National Research Council of Italy, 58022 Follonica, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4511-3557","authenticated-orcid":false,"given":"Giampiero","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3083-6218","authenticated-orcid":false,"given":"Maria Celeste","family":"Dias","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5440-1772","authenticated-orcid":false,"given":"M\u00e1rcia","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre, 4169-007 Porto, Portugal"},{"name":"CITAB, University of Tr\u00e1s-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1589-9074","authenticated-orcid":false,"given":"Sara","family":"Parri","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-4819","authenticated-orcid":false,"given":"Marco","family":"Romi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"}]},{"given":"Claudia","family":"Faleri","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-6013","authenticated-orcid":false,"given":"Claudio","family":"Cantini","sequence":"additional","affiliation":[{"name":"Institute for BioEconomy, National Research Council of Italy, 58022 Follonica, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rugini, E., Mencuccini, M., Biasi, R., and Altamura, M.M. (2005). Olive (Olea europaea L.). Protocol for Somatic Embryogenesis in Woody Plants, Springer.","DOI":"10.1007\/1-4020-2985-3_27"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"435","DOI":"10.17660\/ActaHortic.2002.586.89","article-title":"Physiological and biochemical remarks on environmental stress in olive (Olea europaea L.)","volume":"586","author":"Vitagliano","year":"2002","journal-title":"Acta Hortic."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.envpol.2007.06.012","article-title":"Human health effects of air pollution","volume":"151","author":"Kampa","year":"2008","journal-title":"Environ. Pollut."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"41475","DOI":"10.1038\/srep41475","article-title":"Fewer clouds in the Mediterranean: Consistency of observations and climate simulations","volume":"7","author":"Wild","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/s11104-017-3485-5","article-title":"Effects of UV radiation and rainfall reduction on leaf and soil parameters related to C and N cycles of a Mediterranean shrubland before and after a controlled fire","volume":"424","author":"Verdaguer","year":"2018","journal-title":"Plant Soil"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jplph.2018.01.004","article-title":"UV-B radiation modulates physiology and lipophilic metabolite profile in Olea europaea","volume":"222","author":"Dias","year":"2018","journal-title":"J. Plant Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"112199","DOI":"10.1016\/j.phytochem.2019.112199","article-title":"The antioxidant system in Olea europaea to enhanced UV-B radiation also depends on flavonoids and secoiridoids","volume":"170","author":"Dias","year":"2020","journal-title":"Phytochemistry"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Brito, C., Dinis, L.-T., Moutinho-Pereira, J., and Correia, C.M. (2019). Drought Stress Effects and Olive Tree Acclimation under a Changing Climate. Plants, 8.","DOI":"10.3390\/plants8070232"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S1360-1385(98)01215-1","article-title":"Higher plants and UV-B radiation: Balancing damage, repair and acclimation","volume":"3","author":"Jansen","year":"1998","journal-title":"Trends Plant Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1080\/02648725.2017.1340546","article-title":"Molecular analysis of Hsp70 mechanisms in plants and their function in response to stress","volume":"33","author":"Usman","year":"2017","journal-title":"Biotechnol. Genet. Eng. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.jphotobiol.2013.08.013","article-title":"Enhancement of growth, photosynthetic performance and yield by exclusion of ambient UV components in C3 and C4 plants","volume":"127","author":"Kataria","year":"2013","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Piccini, C., Cai, G., Dias, M.C., Romi, M., Longo, R., and Cantini, C. (2020). UV-B Radiation Affects Photosynthesis-Related Processes of Two Italian Olea europaea (L.) Varieties Differently. Plants, 9.","DOI":"10.3390\/plants9121712"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.plantsci.2017.09.006","article-title":"Developmental reprogramming by UV-B radiation in plants","volume":"264","author":"Dotto","year":"2017","journal-title":"Plant Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s11099-015-0102-9","article-title":"Ultraviolet-B radiation or heat cause changes in photosynthesis, antioxidant enzyme activities and pollen performance in olive tree","volume":"53","author":"Koubouris","year":"2015","journal-title":"Photosynthetica"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"481","DOI":"10.9755\/ejfa.v24i6.481501","article-title":"Plant physiological responses to UV-B radiation","volume":"24","author":"Zlatev","year":"2012","journal-title":"Emir. J. Food Agric."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.envexpbot.2017.05.008","article-title":"Photosynthetic performance and volatile organic compounds profile in Eucalyptus globulus after UVB radiation","volume":"140","author":"Machado","year":"2017","journal-title":"Environ. Exp. Bot."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.jphotobiol.2011.05.004","article-title":"Impact of UV-B irradiation on photosynthetic performance and chloroplast membrane components in Oryza sativa L.","volume":"104","author":"Lidon","year":"2011","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/BF00014600","article-title":"UV-B damage and protection at the molecular level in plants","volume":"39","author":"Strid","year":"1994","journal-title":"Photosynth. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"13430","DOI":"10.1073\/pnas.230451497","article-title":"Rubisco activase constrains the photosynthetic potential of leaves at high temperature and CO2","volume":"97","author":"Salvucci","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.envexpbot.2012.04.001","article-title":"Decreased CO2 availability and inactivation of Rubisco limit photosynthesis in cotton plants under heat and drought stress in the field","volume":"83","author":"Gore","year":"2012","journal-title":"Environ. Exp. Bot."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s10535-010-0102-3","article-title":"UV-B induced stress responses in three rice cultivars","volume":"54","author":"Fedina","year":"2010","journal-title":"Biol. Plant."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/j.1365-3040.1997.00093.x","article-title":"Analysis of limitations to CO2 assimilation on exposure of leaves of two Brassica napus cultivars to UV-B","volume":"20","author":"Allen","year":"1997","journal-title":"Plant Cell Environ."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s004250000313","article-title":"Effects of ultraviolet radiation on photosynthesis and related enzyme reactions of marine macroalgae","volume":"211","author":"Bischof","year":"2000","journal-title":"Planta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1071\/PP00116","article-title":"Effects of growth under UVA radiation on CO2 assimilation, carbon partitioning, PSII photochemistry and resistance to UVB radiation in Brassica napus cv. Topas","volume":"28","author":"Savitch","year":"2001","journal-title":"Funct. Plant Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1093\/jxb\/erm360","article-title":"Co- and post-translational modifications in Rubisco: Unanswered questions","volume":"59","author":"Houtz","year":"2007","journal-title":"J. Exp. Bot."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/1011-1344(90)85189-4","article-title":"Effects of ultraviolet radiation and visible light on growth, fluorescence induction, ultraweak luminescence and peroxidase activity in sugar beet plants","volume":"8","author":"Panagopoulos","year":"1990","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1111\/j.1399-3054.1994.tb03042.x","article-title":"Photooxidative stress in plants","volume":"92","author":"Foyer","year":"1994","journal-title":"Physiol. Plant."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/S0958-1669(98)80118-3","article-title":"Plant resistance to environmental stress","volume":"9","author":"Smirnoff","year":"1998","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_29","first-page":"25","article-title":"The effects of ultraviolet radiation on the contents of chlorophyll, flavonoid, anthocyanin and proline in Capsicum annuum L.","volume":"32","author":"Mahdavian","year":"2008","journal-title":"Turk. J. Bot."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1104\/pp.103.030049","article-title":"Ultraviolet-B Radiation-Mediated Responses in Plants. Balancing Damage and Protection","volume":"133","author":"Frohnmeyer","year":"2003","journal-title":"Plant Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jplph.2017.12.010","article-title":"Selective responses of class III plant peroxidase isoforms to environmentally relevant UV-B doses","volume":"221","author":"Hideg","year":"2018","journal-title":"J. Plant Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.tplants.2012.09.003","article-title":"UV-B exposure, ROS, and stress: Inseparable companions or loosely linked associates?","volume":"18","author":"Hideg","year":"2013","journal-title":"Trends Plant Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.plantsci.2012.07.014","article-title":"Flavonoids as antioxidants in plants: Location and functional significance","volume":"196","author":"Agati","year":"2012","journal-title":"Plant Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1073\/pnas.1117099109","article-title":"Sucrose synthase activity in the sus1\/sus2\/sus3\/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production","volume":"109","author":"Li","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.envexpbot.2010.09.006","article-title":"Effects of low UV-B doses on the accumulation of UV-B absorbing compounds and total phenolics and carbohydrate metabolism in the peel of harvested lemons","volume":"70","author":"Interdonato","year":"2011","journal-title":"Environ. Exp. Bot."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.envexpbot.2018.04.015","article-title":"Ultraviolet B irradiation influences the fruit quality and sucrose metabolism of peach (Prunus persica L.)","volume":"153","author":"Wang","year":"2018","journal-title":"Environ. Exp. Bot."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0300-483X(00)00304-8","article-title":"Applications and pitfalls of stress-proteins in biomonitoring","volume":"153","author":"Bierkens","year":"2000","journal-title":"Toxicology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1007\/s00018-004-4464-6","article-title":"Hsp70 chaperones: Cellular functions and molecular mechanism","volume":"62","author":"Mayer","year":"2005","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1046\/j.1461-0248.2003.00528.x","article-title":"The evolutionary and ecological role of heat shock proteins","volume":"6","author":"Kristensen","year":"2003","journal-title":"Ecol. Lett."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Swindell, W.R., Huebner, M., and Weber, A.P. (2007). Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways. BMC Genom., 8.","DOI":"10.1186\/1471-2164-8-125"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4189","DOI":"10.1021\/pr100504j","article-title":"Tissue-Specific Defense and Thermo-Adaptive Mechanisms of Soybean Seedlings under Heat Stress Revealed by Proteomic Approach","volume":"9","author":"Ahsan","year":"2010","journal-title":"J. Proteome Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.3389\/fpls.2016.01917","article-title":"Transcriptomic Profiling of Soybean in Response to High-Intensity UV-B Irradiation Reveals Stress Defense Signaling","volume":"7","author":"Yoon","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jplph.2017.11.013","article-title":"Tomato plants use non-enzymatic antioxidant pathways to cope with moderate UV-A\/B irradiation: A contribution to the use of UV-A\/B in horticulture","volume":"221","author":"Ponte","year":"2018","journal-title":"J. Plant Physiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.tplants.2011.03.007","article-title":"ROS signaling: The new wave?","volume":"16","author":"Mittler","year":"2011","journal-title":"Trends Plant Sci."},{"key":"ref_45","first-page":"1221","article-title":"Class I and II small heat-shock proteins protect protein translation factors during heat stress","volume":"172","author":"McLoughlin","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ara\u00fajo, M., Santos, C., and Dias, M.C. (2018). Can Young Olive Plants Overcome Heat Shock?. Climate Change Management, Springer Science and Business Media LLC.","DOI":"10.1007\/978-3-319-72874-2_11"},{"key":"ref_47","first-page":"419","article-title":"Heat shock response in olive (Olea europaeaL.) twigs: Identification and analysis of a cDNA coding a class I small heat shock protein","volume":"145","author":"Assab","year":"2011","journal-title":"Plant Biosyst.-Int. J. Deal. All Asp. Plant Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.jphotobiol.2016.06.048","article-title":"Plasticity of young Moringa oleifera L. plants to face water deficit and UVB radiation challenges","volume":"162","author":"Santos","year":"2016","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"921","DOI":"10.3389\/fpls.2017.00921","article-title":"Antioxidant Responses Induced by UVB Radiation in Deschampsia antarctica Desv","volume":"8","author":"Contreras","year":"2017","journal-title":"Front. Plant Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"388","DOI":"10.4161\/psb.4.5.8294","article-title":"Soluble sugars","volume":"4","author":"Rosa","year":"2009","journal-title":"Plant Signal. Behav."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S1360-1385(96)80048-3","article-title":"Mannitol metabolism in plants: A method for coping with stress","volume":"1","author":"Stoop","year":"1996","journal-title":"Trends Plant Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"6805","DOI":"10.3390\/ijms14046805","article-title":"Alternative Oxidase: A Mitochondrial Respiratory Pathway to Maintain Metabolic and Signaling Homeostasis during Abiotic and Biotic Stress in Plants","volume":"14","author":"Vanlerberghe","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0168-9452(99)00260-5","article-title":"Sucrose and the integration of metabolism in vascular plants","volume":"154","author":"Farrar","year":"2000","journal-title":"Plant Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S1360-1385(02)00029-8","article-title":"Origin of sucrose metabolism in higher plants: When, how and why?","volume":"8","author":"Salerno","year":"2003","journal-title":"Trends Plant Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.tplants.2004.10.009","article-title":"Function and regulation of plant invertases: Sweet sensations","volume":"9","author":"Roitsch","year":"2004","journal-title":"Trends Plant Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.pbi.2004.03.014","article-title":"Sucrose metabolism: Regulatory mechanisms and pivotal roles in sugar sensing and plant development","volume":"7","author":"Koch","year":"2004","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"608954","DOI":"10.1100\/2012\/608954","article-title":"Ultraviolet-B Radiation and Nitrogen Affect Nutrient Concentrations and the Amount of Nutrients Acquired by Above-Ground Organs of Maize","volume":"2012","author":"Correia","year":"2012","journal-title":"Sci. World J."},{"key":"ref_58","unstructured":"Forster, P.M., Thompson, D.W.J., Baldwin, M.P., Chipperfield, M.P., Dameris, M., Haigh, J.D., Karoly, D.J., Kushner, P.J., Ran-del, W.J., and Rosenlof, K.H. (2011). Stratospheric Changes and Climate: Scientific Assessment of Ozone Depletion, Global Ozone Research and Monitoring Project, United Nations Environment Programme. Report No. 52."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s10535-005-0048-z","article-title":"Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes","volume":"49","author":"Agarwal","year":"2005","journal-title":"Biol. Plant."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0021-9258(19)50881-X","article-title":"A Spectrophotometric Method for Measuring the Breakdown of Hydrogen Peroxide by Catalase","volume":"195","author":"Beers","year":"1952","journal-title":"J. Biol. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1007\/s004250050524","article-title":"Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds","volume":"207","author":"Hodges","year":"1999","journal-title":"Planta"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/0005-2744(74)90274-5","article-title":"An improved spectrophotometric assay for ribulosebisphosphate carboxylase","volume":"358","author":"Lilley","year":"1974","journal-title":"Biochim. Biophys. Acta BBA-Enzymol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/nprot.2014.022","article-title":"Universal sample preparation method integrating trichloroacetic acid\/acetone precipitation with phenol extraction for crop proteomic analysis","volume":"9","author":"Wu","year":"2014","journal-title":"Nat. Protoc."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1007\/s00425-019-03245-9","article-title":"Distribution of cell-wall polysaccharides and proteins during growth of the hemp hypocotyl","volume":"250","author":"Behr","year":"2019","journal-title":"Planta"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0022-5320(69)90033-1","article-title":"A low-viscosity epoxy resin embedding medium for electron microscopy","volume":"26","author":"Spurr","year":"1969","journal-title":"J. Ultrastruct. Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/20\/11214\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:17:07Z","timestamp":1760167027000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/20\/11214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,18]]},"references-count":65,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["ijms222011214"],"URL":"https:\/\/doi.org\/10.3390\/ijms222011214","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,18]]}}}