{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:35:52Z","timestamp":1777415752441,"version":"3.51.4"},"reference-count":75,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010784","name":"Banco Santander (Spain)","doi-asserted-by":"publisher","award":["Convenio de colaboraci\u00f3n empresarial en actividades de inter\u00e9s general"],"award-info":[{"award-number":["Convenio de colaboraci\u00f3n empresarial en actividades de inter\u00e9s general"]}],"id":[{"id":"10.13039\/100010784","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/01026\/2018"],"award-info":[{"award-number":["CEECIND\/01026\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FI19\/00041"],"award-info":[{"award-number":["FI19\/00041"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CD19\/00048"],"award-info":[{"award-number":["CD19\/00048"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["MS17\/00021"],"award-info":[{"award-number":["MS17\/00021"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CP17\/00021"],"award-info":[{"award-number":["CP17\/00021"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI18\/0911"],"award-info":[{"award-number":["PI18\/0911"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centro de Investigaci\u00f3n en Red en Enfermedades Cardiovasculares","award":["CB16\/11\/00494"],"award-info":[{"award-number":["CB16\/11\/00494"]}]},{"name":"Government of Extremadura","award":["IB20184"],"award-info":[{"award-number":["IB20184"]}]},{"name":"Government of Extremadura","award":["GR18199"],"award-info":[{"award-number":["GR18199"]}]},{"name":"Government of Extremadura","award":["TA18054"],"award-info":[{"award-number":["TA18054"]}]},{"name":"Government of Extremadura","award":["TA18011"],"award-info":[{"award-number":["TA18011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Polypropylene (PP) mesh is well-known as a gold standard of all prosthetic materials of choice for the reinforcement of soft tissues in case of hernia, organ prolapse, and urinary incontinence. The adverse effects that follow surgical mesh implantation remain an unmet medical challenge. Herein, it is outlined a new approach to allow viability and adhesion of human menstrual blood-derived mesenchymal stromal cells (MenSCs) on PP surgical meshes. A multilayered fibrin coating, based on fibrinogen and thrombin from a commercial fibrin sealant, was optimized to guarantee a homogeneous and stratified film on PP mesh. MenSCs were seeded on the optimized fibrin-coated meshes and their adhesion, viability, phenotype, gene expression, and immunomodulatory capacity were fully evaluated. This coating guaranteed MenSC viability, adhesion and did not trigger any change in their stemness and inflammatory profile. Additionally, MenSCs seeded on fibrin-coated meshes significantly decreased CD4+ and CD8+ T cell proliferation, compared to in vitro stimulated lymphocytes (p &lt; 0.0001). Hence, the proposed fibrin coating for PP surgical meshes may allow the local administration of stromal cells and the reduction of the exacerbated inflammatory response following mesh implantation surgery. Reproducible and easy to adapt to other cell types, this method undoubtedly requires a multidisciplinary and translational approach to be improved for future clinical uses.<\/jats:p>","DOI":"10.3390\/ijms222413385","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T01:20:41Z","timestamp":1639444841000},"page":"13385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5965-7718","authenticated-orcid":false,"given":"Federica","family":"Marinaro","sequence":"first","affiliation":[{"name":"Stem Cell Therapy Unit, Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre, 10071 C\u00e1ceres, Spain"}]},{"given":"Joana M.","family":"Silva","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Zona Industrial da Gandra, 4805-017 Barco, Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s-PT Government Associate Laboratory, 4805-017 Guimar\u00e3es, Portugal"}]},{"given":"Alexandre A.","family":"Barros","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Zona Industrial da Gandra, 4805-017 Barco, Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s-PT Government Associate Laboratory, 4805-017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0543-548X","authenticated-orcid":false,"given":"Ivo M.","family":"Aroso","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Zona Industrial da Gandra, 4805-017 Barco, Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s-PT Government Associate Laboratory, 4805-017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4354-5313","authenticated-orcid":false,"given":"Juan C.","family":"G\u00f3mez-Blanco","sequence":"additional","affiliation":[{"name":"Stem Cell Therapy Unit, Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre, 10071 C\u00e1ceres, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4575-8264","authenticated-orcid":false,"given":"Isaac","family":"Jardin","sequence":"additional","affiliation":[{"name":"Cell Physiology Research Group, Department of Physiology, University of Extremadura, 10003 C\u00e1ceres, Spain"},{"name":"Institute of Molecular Pathology Biomarkers, University of Extremadura, 10003 C\u00e1ceres, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5234-1478","authenticated-orcid":false,"given":"Jose J.","family":"Lopez","sequence":"additional","affiliation":[{"name":"Cell Physiology Research Group, Department of Physiology, University of Extremadura, 10003 C\u00e1ceres, Spain"},{"name":"Institute of Molecular Pathology Biomarkers, University of Extremadura, 10003 C\u00e1ceres, Spain"}]},{"given":"Mar\u00eda","family":"Pulido","sequence":"additional","affiliation":[{"name":"Stem Cell Therapy Unit, Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre, 10071 C\u00e1ceres, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8989-2468","authenticated-orcid":false,"given":"Mar\u00eda \u00c1ngeles","family":"de Pedro","sequence":"additional","affiliation":[{"name":"Stem Cell Therapy Unit, Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre, 10071 C\u00e1ceres, Spain"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Zona Industrial da Gandra, 4805-017 Barco, Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s-PT Government Associate Laboratory, 4805-017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2138-988X","authenticated-orcid":false,"given":"Francisco Miguel","family":"S\u00e1nchez-Margallo","sequence":"additional","affiliation":[{"name":"Stem Cell Therapy Unit, Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre, 10071 C\u00e1ceres, Spain"},{"name":"Centro de Investigaci\u00f3n en Red en Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6797-7287","authenticated-orcid":false,"given":"Javier G.","family":"Casado","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology Biomarkers, University of Extremadura, 10003 C\u00e1ceres, Spain"},{"name":"Centro de Investigaci\u00f3n en Red en Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain"},{"name":"Immunology Unit, Department of Physiology, University of Extremadura, 10003 C\u00e1ceres, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5051-5913","authenticated-orcid":false,"given":"Esther","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Stem Cell Therapy Unit, Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre, 10071 C\u00e1ceres, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1557\/mrs.2013.52","article-title":"Ziegler-Natta Catalysis: 50 Years after the Nobel Prize","volume":"38","author":"Claverie","year":"2013","journal-title":"MRS Bull."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S0161-6420(81)80012-7","article-title":"Polypropylene","volume":"88","author":"Clayman","year":"1981","journal-title":"Ophthalmology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/0888-6296(90)90041-D","article-title":"In Vitro Variability in Fentanyl Absorption by Different Membrane Oxygenators","volume":"4","author":"Rosen","year":"1990","journal-title":"J. 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