{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T21:01:32Z","timestamp":1777064492870,"version":"3.51.4"},"reference-count":58,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>REV-ERB\u03b1 (nr1d1, nuclear receptor subfamily 1 group D member 1) is a transcriptional repressor that in mammals regulates nutrient metabolism, and has effects on energy homeostasis, although its role in teleosts is poorly understood. To determine REV-ERB\u03b1\u2019s involvement in fish energy balance and metabolism, we studied the effects of acute and 7-day administration of its agonist SR9009 on food intake, weight and length gain, locomotor activity, feeding regulators, plasma and hepatic metabolites, and liver enzymatic activity. SR9009 inhibited feeding, lowering body weight and length gain. In addition, the abundance of ghrelin mRNA decreased in the intestine, and abundance of leptin-aI mRNA increased in the liver. Hypocretin, neuropeptide y (npy), and proopiomelanocortin (pomc) mRNA abundance was not modified after acute or subchronic SR9009 administration, while hypothalamic cocaine- and amphetamine-regulated transcript (cartpt-I) was induced in the subchronic treatment, being a possible mediator of the anorectic effects. Moreover, SR9009 decreased plasma glucose, coinciding with increased glycolysis and a decreased gluconeogenesis in the liver. Decreased triglyceride levels and activity of lipogenic enzymes suggest a lipogenesis reduction by SR9009. Energy expenditure by locomotor activity was not significantly affected by SR9009. Overall, this study shows for the first time in fish the effects of REV-ERB\u03b1 activation via SR9009, promoting a negative energy balance by reducing energetic inputs and regulating lipid and glucose metabolism.<\/jats:p>","DOI":"10.3390\/ijms23062921","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T12:35:37Z","timestamp":1646742937000},"page":"2921","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["REV-ERB\u03b1 Agonist SR9009 Promotes a Negative Energy Balance in Goldfish"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6629-2507","authenticated-orcid":false,"given":"Nuria","family":"Saiz","sequence":"first","affiliation":[{"name":"Fish Neuroendocrinology Group, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain"}]},{"given":"Lisbeth","family":"Herrera-Castillo","sequence":"additional","affiliation":[{"name":"Fish Neuroendocrinology Group, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain"}]},{"given":"Esther","family":"Isorna","sequence":"additional","affiliation":[{"name":"Fish Neuroendocrinology Group, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1185-9377","authenticated-orcid":false,"given":"Mar\u00eda Jes\u00fas","family":"Delgado","sequence":"additional","affiliation":[{"name":"Fish Neuroendocrinology Group, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain"}]},{"given":"Marta","family":"Conde-Sieira","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Mari\u00f1a, Laboratorio de Fisiolox\u00eda Animal, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, 36310 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6847-3993","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"Soengas","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Mari\u00f1a, Laboratorio de Fisiolox\u00eda Animal, Departamento de Biolox\u00eda Funcional e Ciencias da Sa\u00fade, Facultade de Biolox\u00eda, Universidade de Vigo, 36310 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1971-6792","authenticated-orcid":false,"given":"Nuria","family":"de Pedro","sequence":"additional","affiliation":[{"name":"Fish Neuroendocrinology Group, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/j.tem.2014.06.011","article-title":"Nuclear receptor Rev-erb\u03b1: Up, down, and all around","volume":"25","author":"Everett","year":"2014","journal-title":"Trends Endocrinol. 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