{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T23:04:37Z","timestamp":1768259077670,"version":"3.49.0"},"reference-count":54,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["FK 125035"],"award-info":[{"award-number":["FK 125035"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"name":"J\u00e1nos Bolyai Scholarship of the Hungarian Academy of Sciences","award":["BO\/00369\/17\/5"],"award-info":[{"award-number":["BO\/00369\/17\/5"]}]},{"name":"New National Excelence Program of the Ministry of Human Capacities","award":["\u00daNKP-19-4-DE-3"],"award-info":[{"award-number":["\u00daNKP-19-4-DE-3"]}]},{"name":"Hungarian Brain Research Program","award":["2017-1.2.1-NKP-2017-00002"],"award-info":[{"award-number":["2017-1.2.1-NKP-2017-00002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Burn injury is a trauma resulting in tissue degradation and severe pain, which is processed first by neuronal circuits in the spinal dorsal horn. We have recently shown that in mice, excitatory dynorphinergic (Pdyn) neurons play a pivotal role in the response to burn-injury-associated tissue damage via histone H3.1 phosphorylation-dependent signaling. As Pdyn neurons were mostly associated with mechanical allodynia, their involvement in thermonociception had to be further elucidated. Using a custom-made AAV9_mutH3.1 virus combined with the CRISPR\/cas9 system, here we provide evidence that blocking histone H3.1 phosphorylation at position serine 10 (S10) in spinal Pdyn neurons significantly increases the thermal nociceptive threshold in mice. In contrast, neither mechanosensation nor acute chemonociception was affected by the transgenic manipulation of histone H3.1. These results suggest that blocking rapid epigenetic tagging of S10H3 in spinal Pdyn neurons alters acute thermosensation and thus explains the involvement of Pdyn cells in the immediate response to burn-injury-associated tissue damage.<\/jats:p>","DOI":"10.3390\/ijms23063178","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T03:34:13Z","timestamp":1647401653000},"page":"3178","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["CRISPR\/Cas9-Based Mutagenesis of Histone H3.1 in Spinal Dynorphinergic Neurons Attenuates Thermal Sensitivity in Mice"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5032-8520","authenticated-orcid":false,"given":"Zolt\u00e1n","family":"M\u00e9sz\u00e1r","sequence":"first","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"given":"\u00c9va","family":"K\u00f3kai","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"given":"Rita","family":"Varga","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2753-5748","authenticated-orcid":false,"given":"L\u00e1szl\u00f3","family":"Ducza","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"given":"Tam\u00e1s","family":"Papp","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8610-3788","authenticated-orcid":false,"given":"Monika","family":"B\u00e9resov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"given":"Marianna","family":"Nagy","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4635-6427","authenticated-orcid":false,"given":"P\u00e9ter","family":"Sz\u00fccs","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4248-1338","authenticated-orcid":false,"given":"Angelika","family":"Varga","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1744-8069-10-59","article-title":"Epigenetic regulation of spinal cord gene expression controls opioid-induced hyperalgesia","volume":"10","author":"Liang","year":"2014","journal-title":"Mol. 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