{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T02:30:12Z","timestamp":1774492212145,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T00:00:00Z","timestamp":1647475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Toxoplasmosis is a highly prevalent human disease, and virulent strains of this parasite emerge from wild biotopes. Here, we report on the potential of a histone deacetylase (HDAC) inhibitor we previously synthesized, named JF363, to act in vitro against a large panel of Toxoplasma strains, as well as against the liver and blood stages of Plasmodium parasites, the causative agents of malaria. In vivo administration of the drug significantly increases the survival of mice during the acute phase of infection by T. gondii, thus delaying its spreading. We further provide evidence of the compound\u2019s efficiency in controlling the formation of cysts in the brain of T. gondii-infected mice. A convincing docking of the JF363 compound in the active site of the five annotated ME49 T. gondii HDACs was performed by extensive sequence\u2013structure comparison modeling. The resulting complexes show a similar mode of binding in the five paralogous structures and a quite similar prediction of affinities in the micromolar range. Altogether, these results pave the way for further development of this compound to treat acute and chronic toxoplasmosis. It also shows promise for the future development of anti-Plasmodium therapeutic interventions.<\/jats:p>","DOI":"10.3390\/ijms23063254","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T21:30:14Z","timestamp":1647811814000},"page":"3254","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Histone Deacetylase (HDAC) Inhibitor with Pleiotropic In Vitro Anti-Toxoplasma and Anti-Plasmodium Activities Controls Acute and Chronic Toxoplasma Infection in Mice"],"prefix":"10.3390","volume":"23","author":[{"given":"Delphine","family":"Jublot","sequence":"first","affiliation":[{"name":"CNRS UMR5525 TIMC-IMAG, Team BNI, Grenoble Alpes, CNRS, Grenoble INP, 38000 Grenoble, France"},{"name":"INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences, Team ApicoLipid, Universit\u00e9 Grenoble Alpes, 38000 Grenoble, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5559-8634","authenticated-orcid":false,"given":"Pierre","family":"Cavaill\u00e8s","sequence":"additional","affiliation":[{"name":"CNRS UMR5525 TIMC-IMAG, Team BNI, Grenoble Alpes, CNRS, Grenoble INP, 38000 Grenoble, France"},{"name":"INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences, Team ApicoLipid, Universit\u00e9 Grenoble Alpes, 38000 Grenoble, France"}]},{"given":"Salima","family":"Kamche","sequence":"additional","affiliation":[{"name":"CNRS UMR5525 TIMC-IMAG, Team BNI, Grenoble Alpes, CNRS, Grenoble INP, 38000 Grenoble, France"},{"name":"CNRS UMR5525 TIMC-IMAG, Team TrEE, Grenoble Alpes, CNRS, Grenoble INP, 38185 Grenoble, France"}]},{"given":"Denise","family":"Francisco","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0046-648X","authenticated-orcid":false,"given":"Diana","family":"Fontinha","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1746-6029","authenticated-orcid":false,"given":"Miguel","family":"Prud\u00eancio","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, Portugal"}]},{"given":"Jean-Francois","family":"Guichou","sequence":"additional","affiliation":[{"name":"Centre de Biologie Structurale (CBS), INSERM, CNRS, Universit\u00e9 de Montpellier, 34000 Montpellier, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6861-3300","authenticated-orcid":false,"given":"Gilles","family":"Labesse","sequence":"additional","affiliation":[{"name":"Centre de Biologie Structurale (CBS), INSERM, CNRS, Universit\u00e9 de Montpellier, 34000 Montpellier, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0034-9291","authenticated-orcid":false,"given":"Denis","family":"Sereno","sequence":"additional","affiliation":[{"name":"Parasite Infectiology Research Group, Institut de Recherche Pour le D\u00e9veloppement, Universit\u00e9 de Montpellier, InterTryp, 34032 Montpellier, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6474-4453","authenticated-orcid":false,"given":"Corinne","family":"Loeuillet","sequence":"additional","affiliation":[{"name":"CNRS UMR5525 TIMC-IMAG, Team BNI, Grenoble Alpes, CNRS, Grenoble INP, 38000 Grenoble, France"},{"name":"INSERM U1209, CNRS UMR 5309, Institute for Advanced Biosciences, Team Genetics Epigenetics and Therapies of Infertility, Universit\u00e9 Grenoble Alpes, 38000 Grenoble, France"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2021, December 20). 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