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By challenging the mother\u2013fetus unit, gestational exercise (GE) favorably modulates deleterious stimuli, such as high-fat, high-sucrose (HFHS) diet-induced adverse consequences for offspring. We aimed to analyze whether GE alters maternal HFHS-consumption effects on male offspring\u2019s maximal workload performance (MWP) and in some skeletal muscle (the soleus\u2014SOL and the tibialis anterior\u2014TA) biomarkers associated with mitochondrial biogenesis and oxidative fitness. Infant male Sprague-Dawley rats were divided into experimental groups according to mothers\u2019 dietary and\/or exercise conditions: offspring of sedentary control diet-fed or HFHS-fed mothers (C\u2013S or HFHS\u2013S, respectively) and of exercised HFHS-fed mothers (HFHS\u2013E). Although maternal HFHS did not significantly alter MWP, offspring from GE dams exhibited increased MWP. Lower SOL AMPk levels in HFHS\u2013S were reverted by GE. SOL PGC-1\u03b1, OXPHOS C-I and C-IV subunits remained unaltered by maternal diet, although increased in HFHS\u2013E offspring. Additionally, GE prevented maternal diet-related SOL miR-378a overexpression, while upregulated miR-34a expression. Decreased TA C-IV subunit expression in HFHS\u2013S was reverted in HFHS\u2013E, concomitantly with the downregulation of miR-338. In conclusion, GE in HFHS-fed dams increases the offspring\u2019s MWP, which seems to be associated with the intrauterine modulation of SM mitochondrial density and functional markers.<\/jats:p>","DOI":"10.3390\/ijms23073916","type":"journal-article","created":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T21:22:39Z","timestamp":1648848159000},"page":"3916","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Gestational Exercise Increases Male Offspring\u2019s Maximal Workload Capacity Early in Life"],"prefix":"10.3390","volume":"23","author":[{"given":"Jorge","family":"Beleza","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Physiology & Immunology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain"}]},{"given":"Jelena","family":"Stevanovi\u0107-Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolism and Exercise (LaMetEx), Research Centre in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, 4200-450 Porto, Portugal"}]},{"given":"Pedro","family":"Coxito","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolism and Exercise (LaMetEx), Research Centre in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, 4200-450 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5447-615X","authenticated-orcid":false,"given":"Hugo","family":"Rocha","sequence":"additional","affiliation":[{"name":"Newborn Screening, Metabolism and Genetics Unit, Human Genetics Department, National Institute of Health Dr Ricardo Jorge, 4000-053 Porto, Portugal"}]},{"given":"Paulo","family":"Santos","sequence":"additional","affiliation":[{"name":"Faculty of Sport, University of Porto, 4200-450 Porto, Portugal"}]},{"given":"Ant\u00f3nio","family":"Ascens\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolism and Exercise (LaMetEx), Research Centre in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, 4200-450 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6314-6523","authenticated-orcid":false,"given":"Joan","family":"Ramon Torrella","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Physiology & Immunology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4808-8374","authenticated-orcid":false,"given":"Jos\u00e9","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolism and Exercise (LaMetEx), Research Centre in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, 4200-450 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1159\/000115355","article-title":"Developmental origins of obesity and the metabolic syndrome: The role of maternal obesity","volume":"36","author":"Armitage","year":"2008","journal-title":"Front. 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