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They accumulate iron in the form of a ferrihydrite mineral core and are capable of binding to and compacting DNA, forming low- and high-order condensates. This dual activity is designed to protect DNA from oxidative stress, resulting from Fenton chemistry or radiation exposure. In most Dps proteins, the DNA-binding properties stem from the N-terminal tail extensions. We explored the structural characteristics of a Dps from Deinococcus grandis that exhibits an atypically long N-terminal tail composed of 52 residues and probed the impact of the ionic strength on protein conformation using size exclusion chromatography, dynamic light scattering, synchrotron radiation circular dichroism and small-angle X-ray scattering. A novel high-spin ferrous iron-binding site was identified in the N-terminal tails, using M\u00f6ssbauer spectroscopy. Our data reveals that the N-terminal tails are structurally dynamic and alter between compact and extended conformations, depending on the ionic strength of the buffer. This prompts the search for other physiologically relevant modulators of tail conformation and hints that the DNA-binding properties of Dps proteins may be affected by external factors.<\/jats:p>","DOI":"10.3390\/ijms23094871","type":"journal-article","created":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T12:06:01Z","timestamp":1651147561000},"page":"4871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5441-9446","authenticated-orcid":false,"given":"Jo\u00e3o P. L.","family":"Guerra","sequence":"first","affiliation":[{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Clement E.","family":"Blanchet","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Hamburg Outstation, Notkestrasse 85, 22603 Hamburg, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6536-9875","authenticated-orcid":false,"given":"Bruno J. C.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias e Tecnologias Nucleares, DECN, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Estrada Nacional 10, 2695-066 Bobadela, Portugal"}]},{"given":"Ana V.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6171-4099","authenticated-orcid":false,"given":"Jo\u00e3o C.","family":"Waerenborgh","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias e Tecnologias Nucleares, DECN, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Estrada Nacional 10, 2695-066 Bobadela, Portugal"}]},{"given":"Nykola C.","family":"Jones","sequence":"additional","affiliation":[{"name":"ISA, Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8018-5433","authenticated-orcid":false,"given":"S\u00f8ren V.","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"ISA, Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7398-2661","authenticated-orcid":false,"given":"Pedro","family":"Tavares","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5567-6073","authenticated-orcid":false,"given":"Alice S.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/978-3-030-58971-4_3","article-title":"An Overview of Dps: Dual Acting Nanovehicles in Prokaryotes with DNA Binding and Ferroxidation Properties","volume":"Volume 96","author":"Harris","year":"2021","journal-title":"Subcellular Biochemistry"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"214187","DOI":"10.1016\/j.ccr.2021.214187","article-title":"Miniferritins: Small multifunctional protein cages","volume":"449","author":"Guerra","year":"2021","journal-title":"Coord. 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