{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T15:31:58Z","timestamp":1774020718118,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T00:00:00Z","timestamp":1654128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund (ERDF)","award":["CENTRO-01-0145-FEDER-000012"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000012"]}]},{"name":"European Regional Development Fund (ERDF)","award":["IF\/01007\/2015"],"award-info":[{"award-number":["IF\/01007\/2015"]}]},{"name":"European Regional Development Fund (ERDF)","award":["POCI-01-0145-FEDER-30916"],"award-info":[{"award-number":["POCI-01-0145-FEDER-30916"]}]},{"name":"European Regional Development Fund (ERDF)","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}]},{"name":"European Regional Development Fund (ERDF)","award":["UIDP\/04539\/2020"],"award-info":[{"award-number":["UIDP\/04539\/2020"]}]},{"name":"European Regional Development Fund (ERDF)","award":["UIDB\/EQU\/00102\/2020"],"award-info":[{"award-number":["UIDB\/EQU\/00102\/2020"]}]},{"name":"European Regional Development Fund (ERDF)","award":["2020.00186.CEECIND"],"award-info":[{"award-number":["2020.00186.CEECIND"]}]},{"DOI":"10.13039\/501100001871","name":"COMPETE 2020\u2014Operational Programme for Competitiveness and Internationalisation","doi-asserted-by":"publisher","award":["CENTRO-01-0145-FEDER-000012"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"COMPETE 2020\u2014Operational Programme for Competitiveness and Internationalisation","doi-asserted-by":"publisher","award":["IF\/01007\/2015"],"award-info":[{"award-number":["IF\/01007\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"COMPETE 2020\u2014Operational Programme for Competitiveness and Internationalisation","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-30916"],"award-info":[{"award-number":["POCI-01-0145-FEDER-30916"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"COMPETE 2020\u2014Operational Programme for Competitiveness and Internationalisation","doi-asserted-by":"publisher","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"COMPETE 2020\u2014Operational Programme for Competitiveness and Internationalisation","doi-asserted-by":"publisher","award":["UIDP\/04539\/2020"],"award-info":[{"award-number":["UIDP\/04539\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"COMPETE 2020\u2014Operational Programme for Competitiveness and Internationalisation","doi-asserted-by":"publisher","award":["UIDB\/EQU\/00102\/2020"],"award-info":[{"award-number":["UIDB\/EQU\/00102\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"COMPETE 2020\u2014Operational Programme for Competitiveness and Internationalisation","doi-asserted-by":"publisher","award":["2020.00186.CEECIND"],"award-info":[{"award-number":["2020.00186.CEECIND"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FCT","award":["CENTRO-01-0145-FEDER-000012"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000012"]}]},{"name":"FCT","award":["IF\/01007\/2015"],"award-info":[{"award-number":["IF\/01007\/2015"]}]},{"name":"FCT","award":["POCI-01-0145-FEDER-30916"],"award-info":[{"award-number":["POCI-01-0145-FEDER-30916"]}]},{"name":"FCT","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}]},{"name":"FCT","award":["UIDP\/04539\/2020"],"award-info":[{"award-number":["UIDP\/04539\/2020"]}]},{"name":"FCT","award":["UIDB\/EQU\/00102\/2020"],"award-info":[{"award-number":["UIDB\/EQU\/00102\/2020"]}]},{"name":"FCT","award":["2020.00186.CEECIND"],"award-info":[{"award-number":["2020.00186.CEECIND"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In recent years, mesoporous silica particles have been revealed as promising drug delivery systems combining high drug loading capacity, excellent biocompatibility, and easy and affordable synthetic and post-synthetic procedures. In fact, the straightforward functionalization approaches of these particles allow their conjugation with targeting moieties in order to surpass one of the major challenges in drug administration, the absence of targeting ability of free drugs that reduces their therapeutic efficacy and causes undesired side effects. In this context, the main goal of this work was to develop a new targeted mesoporous silica nanoparticle formulation with the capability to specifically and efficiently deliver an anticancer drug to hepatocellular carcinoma (HCC) cells. To this purpose, and as proof of concept, we developed redox-responsive mesoporous silica nanoparticles functionalized with the targeting ligand triantennary N-acetylgalactosamine (GalNAc) cluster, which has high affinity to asialoglycoprotein receptors overexpressed in HCC cells, and loaded them with epirubicin, an anthracycline drug. The produced nanocarrier exhibits suitable physicochemical properties for drug delivery, high drug loading capacity, high biocompatibility, and targeting ability to HCC cells, revealing its biopharmaceutical potential as a targeted drug carrier for therapeutic applications in liver diseases.<\/jats:p>","DOI":"10.3390\/ijms23116243","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T11:05:02Z","timestamp":1654167902000},"page":"6243","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Triantennary GalNAc-Functionalized Multi-Responsive Mesoporous Silica Nanoparticles for Drug Delivery Targeted at Asialoglycoprotein Receptor"],"prefix":"10.3390","volume":"23","author":[{"given":"Rosemeyre","family":"Cordeiro","sequence":"first","affiliation":[{"name":"CNC\u2014Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Polo I, 3004-517 Coimbra, Portugal"},{"name":"Institute for Interdisciplinary Research (IIIUC), University of Coimbra, 3030-789 Coimbra, Portugal"}]},{"given":"Ana","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CNC\u2014Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Polo I, 3004-517 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3336-2449","authenticated-orcid":false,"given":"Lu\u00edsa","family":"Dur\u00e3es","sequence":"additional","affiliation":[{"name":"CIEPQPF, Department of Chemical Engineering, University of Coimbra, Rua S\u00edlvio Lima, Polo II, 3030-790 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5554-7829","authenticated-orcid":false,"given":"Henrique","family":"Faneca","sequence":"additional","affiliation":[{"name":"CNC\u2014Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Polo I, 3004-517 Coimbra, Portugal"},{"name":"Institute for Interdisciplinary Research (IIIUC), University of Coimbra, 3030-789 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1800020","DOI":"10.1002\/adbi.201800020","article-title":"Mesoporous silica nanoparticles for targeted and stimuli-responsive delivery of chemotherapeutics: A review","volume":"2","author":"Rahikkala","year":"2018","journal-title":"Adv. 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