{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:00:03Z","timestamp":1777680003776,"version":"3.51.4"},"reference-count":89,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT\/MEC (PIDDAC)","doi-asserted-by":"publisher","award":["UIDB\/50011\/2020"],"award-info":[{"award-number":["UIDB\/50011\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT\/MEC (PIDDAC)","doi-asserted-by":"publisher","award":["UIDP\/50011\/2020"],"award-info":[{"award-number":["UIDP\/50011\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT\/MEC (PIDDAC)","doi-asserted-by":"publisher","award":["LA\/P\/0006\/2020"],"award-info":[{"award-number":["LA\/P\/0006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT\/MEC (PIDDAC)","doi-asserted-by":"publisher","award":["2021.03348.CEECIND"],"award-info":[{"award-number":["2021.03348.CEECIND"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Employment Stimulus","award":["UIDB\/50011\/2020"],"award-info":[{"award-number":["UIDB\/50011\/2020"]}]},{"name":"Scientific Employment Stimulus","award":["UIDP\/50011\/2020"],"award-info":[{"award-number":["UIDP\/50011\/2020"]}]},{"name":"Scientific Employment Stimulus","award":["LA\/P\/0006\/2020"],"award-info":[{"award-number":["LA\/P\/0006\/2020"]}]},{"name":"Scientific Employment Stimulus","award":["2021.03348.CEECIND"],"award-info":[{"award-number":["2021.03348.CEECIND"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The chemical composition, investigated by gas chromatography-mass spectrometry, and antibacterial activity of lipophilic extractives of three varieties of Opuntia ficus-indica roots from Algeria are reported in this paper for the first time. The results obtained revealed a total of 55 compounds, including fatty acids, sterols, monoglycerides and long chain aliphatic alcohols that were identified and quantified. \u03b2-Sitosterol was found as the major compound of the roots of the three varieties. Furthermore, considerable amounts of essential fatty acids (\u03c93, \u03c96, and \u03c99) such as oleic, linoleic, and linolenic acids were also identified. The green variety was the richest among the three studied varieties. The antibacterial activity, evaluated with disc diffusion method, revealed that lipophilic extracts were effective mainly against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) (19~23 mm). Gram-negative strains mainly Pseudomonas aeruginosa gave an inhibition zone of 18 mm, which is considered high antibacterial activity. The minimal inhibitory concentrations of the tested bacteria revealed interesting values against the majority of bacteria tested: 75\u2013100 \u00b5g mL\u22121 for Bacillus sp., 250\u2013350 \u00b5g\/mL for the two Staphylococcus strains, 550\u2013600 \u00b5g mL\u22121 for E. coli, and 750\u2013950 \u00b5g mL\u22121 obtained with Pseudomonas sp. This study allows us to conclude that the lipophilic fractions of cactus roots possess interesting phytochemicals such as steroids, some fatty acids and long chain alcohols that acted as antibiotic-like compounds countering pathogenic strains.<\/jats:p>","DOI":"10.3390\/ijms231911161","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T21:10:05Z","timestamp":1663881005000},"page":"11161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Lipophilic Compounds and Antibacterial Activity of Opuntia ficus-indica Root Extracts from Algeria"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2597-2549","authenticated-orcid":false,"given":"Elias","family":"Benramdane","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biologie Physico-Chimique, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Bejaia 06000, Algeria"},{"name":"CICECO\u2014Aveiro Institute of Materials and Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Nadia","family":"Chougui","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Sciences Alimentaires, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Bejaia 06000, Algeria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1922-6937","authenticated-orcid":false,"given":"Patr\u00edcia A. B.","family":"Ramos","sequence":"additional","affiliation":[{"name":"CICECO\u2014Aveiro Institute of Materials and Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Nawal","family":"Makhloufi","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie Physico-Chimique, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Bejaia 06000, Algeria"}]},{"given":"Abderezak","family":"Tamendjari","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Bejaia 06000, Algeria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-8416","authenticated-orcid":false,"given":"Armando J. D.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"CICECO\u2014Aveiro Institute of Materials and Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6749-7619","authenticated-orcid":false,"given":"S\u00f3nia A. O.","family":"Santos","sequence":"additional","affiliation":[{"name":"CICECO\u2014Aveiro Institute of Materials and Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8657","DOI":"10.1007\/s00253-019-10125-5","article-title":"Cacti for production of metabolites: Current state and perspectives","volume":"103","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.foodres.2018.06.047","article-title":"Opuntia (Cactaceae) plant compounds, biological activities and prospects\u2013A comprehensive review","volume":"112","author":"Aruwa","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/978-3-030-31885-7_26","article-title":"Wild Fruits: Composition, Nutritional Value and Products","volume":"30","author":"Tahir","year":"2019","journal-title":"Wild Fruits Compos. Nutr. Value Prod."},{"key":"ref_4","first-page":"15","article-title":"Pharmacological actions of Opuntia ficus indica: A review","volume":"2","author":"Kaur","year":"2012","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"383","DOI":"10.17660\/ActaHortic.2009.811.52","article-title":"Cactus roots: Depth and sensitivity to extreme temperatures","volume":"811","author":"Nobel","year":"2009","journal-title":"Acta Hortic."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"224","DOI":"10.2134\/agronj1991.00021962008300010052x","article-title":"Growth and Predicted Productivity of Opuntia ficus-indica for Current and Elevated Carbon Dioxide","volume":"83","author":"Nobel","year":"1991","journal-title":"Agron. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1046\/j.1469-8137.1998.00884.x","article-title":"Root growth, developmental changes in the apex, and hydraulic conductivity for Opuntia ficus-indica during drought","volume":"138","author":"Dubrovsky","year":"1998","journal-title":"New Phytol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.foodres.2018.03.062","article-title":"Phytochemical characterization of different prickly pear (Opuntia ficus-indica (L.) Mill.) cultivars and botanical parts: UHPLC-ESI-MSn metabolomics profiles and their chemometric analysis","volume":"108","author":"Mena","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.foodchem.2018.11.031","article-title":"An integral profile of bioactive compounds and functional properties of prickly pear (Opuntia ficus indica L.) peel with different tonalities Diana","volume":"278","year":"2019","journal-title":"Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1021\/jf048751y","article-title":"Color, Betalain Pattern, and Antioxidant Properties of Cactus Pear (Opuntia spp.) Clones","volume":"53","author":"Stintzing","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"299","DOI":"10.5897\/AJB12.1946","article-title":"Physico-chemical characterisation and antioxidant activity of some Opuntia ficus-indica varieties grown in North Algeria","volume":"12","author":"Chougui","year":"2013","journal-title":"Afr. J. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6895","DOI":"10.1021\/jf025696p","article-title":"Antioxidant Activities of Sicilian Prickly Pear (Opuntia ficus indica) Fruit Extracts and Reducing Properties of Its Betalains: Betanin and Indicaxanthin","volume":"50","author":"Butera","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/j.foodchem.2013.01.054","article-title":"Oil composition and characterisation of phenolic compounds of Opuntia ficus-indica seeds","volume":"139","author":"Chougui","year":"2013","journal-title":"Food Chem."},{"key":"ref_14","first-page":"85","article-title":"Oil content and main constituents of cactus seed oils Opuntia Ficus Indica of different origin in Morocco","volume":"8","author":"Taoufik","year":"2015","journal-title":"Med. J. Nutr. Metab."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kolniak-Ostek, J., Kita, A., Miedzianka, J., Andreu-Coll, L., Legua, P., and Hernandez, F. (2020). Characterization of Bioactive Compounds of Opuntia ficus-indica (L.) Mill. Seeds from Spanish Cultivars. Molecules, 25.","DOI":"10.3390\/molecules25235734"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.jfca.2009.12.003","article-title":"Proximate composition, phenolic acids, and flavonoids characterization of commercial and wild nopal ({Opuntia} spp.)","volume":"23","author":"Mortensen","year":"2010","journal-title":"J. Food Compos. Anal."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.phytol.2009.11.004","article-title":"Profiling the chemical content of Opuntia ficus-indica flowers by HPLC\u2013PDA-ESI-MS and GC\/EIMS analyses","volume":"3","author":"Pawlowska","year":"2010","journal-title":"Phytochem. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.indcrop.2017.12.028","article-title":"Anti-inflammatory activity and phenolic composition of prickly pear ({Opuntia} ficus-indica) flowers","volume":"112","author":"Ammar","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/ajcn\/80.2.391","article-title":"Supplementation with cactus pear (Opuntia ficus-indica) fruit decreases oxidative stress in healthy humans: A comparative study with vitamin C","volume":"80","author":"Tesoriere","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1002\/ptr.2075","article-title":"Opuntia ficus indica (L.) Mill, mucilages show cytoprotective effect on gastric mucosa in rat","volume":"21","author":"Galati","year":"2007","journal-title":"Phyther. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.indcrop.2014.08.046","article-title":"Phenolic composition, antioxidant and anti-inflammatory activities of extracts from Moroccan Opuntia ficus-indica flowers obtained by different extraction methods","volume":"62","author":"Benayad","year":"2014","journal-title":"Ind. Crops Prod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1017\/S0007114513002663","article-title":"Indicaxanthin inhibits NADPH oxidase (NOX)-1 activation and NF-\u03baB-dependent release of inflammatory mediators and prevents the increase of epithelial permeability in IL-1\u03b2-exposed Caco-2 cells","volume":"111","author":"Tesoriere","year":"2014","journal-title":"Br. J. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.phymed.2018.09.171","article-title":"Indicaxanthin from Opuntia Ficus Indica (L. Mill) impairs melanoma cell proliferation, invasiveness, and tumor progression","volume":"50","author":"Allegra","year":"2018","journal-title":"Phytomedicine"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.foodres.2013.08.043","article-title":"Evaluation of Opuntia spp. derived products as antiproliferative agents in human colon cancer cell line (HT29)","volume":"54","author":"Serra","year":"2013","journal-title":"Food Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108734","DOI":"10.1016\/j.cbi.2019.108734","article-title":"Isorhamnetin glycoside isolated from Opuntia ficus-indica (L.) MilI induces apoptosis in human colon cancer cells through mitochondrial damage","volume":"310","year":"2019","journal-title":"Chem. Biol. Interact."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.etap.2011.08.007","article-title":"Evaluation of antioxidant and antiulcerogenic activities of {Opuntia} ficus indica f. inermis flowers extract in rats","volume":"32","author":"Alimi","year":"2011","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1007\/s10068-011-0177-4","article-title":"Inhibitory activity of aromadendrin from prickly pear (Opuntia ficus-indica) root on aldose reductase and the formation of advanced glycation end products","volume":"20","author":"Jeon","year":"2011","journal-title":"Food Sci. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.3390\/toxins2071751","article-title":"Food Poisoning and Staphylococcus aureus Enterotoxins","volume":"2","author":"Mendoza","year":"2010","journal-title":"Toxins"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s100960100455","article-title":"Frequency of Isolation of Pathogens from Bloodstream, Nosocomial Pneumonia, Skin and Soft Tissue, and Urinary Tract Infections Occurring in European Patients and the European SENTRY Participant Group","volume":"20","author":"Fluit","year":"2001","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Manabe Id, T., Fujikura, Y., Mizukami, K., Akatsu, H., and Kudo, K. (2019). Pneumonia-associated death in patients with dementia: A systematic review and meta-analysis. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0213825"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1002\/jsfa.8628","article-title":"Antioxidant properties and chemical characterization of Spanish Opuntia ficus-indica Mill. cladodes and fruits","volume":"98","author":"Andreu","year":"2018","journal-title":"J. Sci. Food Agric."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.tplants.2014.01.006","article-title":"Engineering crassulacean acid metabolism to improve water-use efficiency","volume":"19","author":"Borland","year":"2014","journal-title":"Trends Plant Sci."},{"key":"ref_33","first-page":"20","article-title":"Morphological and physicochemical diversity of prickly pears in Bejaia-Algeria","volume":"7","author":"Chougui","year":"2016","journal-title":"Int. J. Sci. Eng. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1007\/s13197-019-04127-4","article-title":"Impact of particle size on functional, physicochemical properties and antioxidant activity of cladode powder (Opuntia ficus-indica)","volume":"57","author":"Nabil","year":"2020","journal-title":"J. Food Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.lwt.2015.08.044","article-title":"Fruit and juice characteristics of 30 accessions of two cactus pear species (Opuntia ficus indica and Opuntia megacantha) from different regions of Morocco","volume":"65","author":"Mazri","year":"2016","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_36","first-page":"221","article-title":"Organic fertilization and plant population affect shoot and root biomass of forage cactus pear (Opuntia ficus-indica mill.)","volume":"995","author":"Silva","year":"2013","journal-title":"Acta Hortic."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/j.indcrop.2015.07.055","article-title":"Polar and lipophilic extracts characterization of roots, stalks, leaves and flowers of water hyacinth (Eichhornia crassipes), and insights for its future valorization","volume":"76","author":"Silva","year":"2015","journal-title":"Ind. Crops Prod."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Rocchetti, G., Pellizzoni, M., Montesano, D., and Lucini, L. (2018). Italian opuntia ficus-indica cladodes as rich source of bioactive compounds with health-promoting properties. Foods, 7.","DOI":"10.3390\/foods7020024"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0308-8146(03)00128-6","article-title":"Recovered lipids from prickly pear [Opuntia ficus-indica (L.) Mill] peel: A good source of polyunsaturated fatty acids, natural antioxidant vitamins and sterols","volume":"83","author":"Ramadan","year":"2003","journal-title":"Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"196","DOI":"10.2174\/157340131003140828121015","article-title":"Phytochemical Content, Nutraceutical Potential and Biotechnological Applications of an Ancient Mexican Plant: Nopal (Opuntia ficus-indica)","volume":"10","year":"2014","journal-title":"Curr. Nutr. Food Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1467-3010.2007.00623.x","article-title":"The health effects of dietary unsaturated fatty acids","volume":"32","author":"Bentley","year":"2007","journal-title":"Nutr. Bull."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.idairyj.2009.01.001","article-title":"Has the association between saturated fatty acids, serum cholesterol and coronary heart disease been over emphasized?","volume":"19","author":"Parodi","year":"2009","journal-title":"Int. Dairy J."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Briggs, M.A., Petersen, K.S., and Kris-Etherton, P.M. (2017). Saturated Fatty Acids and Cardiovascular Disease: Replacements for Saturated Fat to Reduce Cardiovascular Risk. Healthcare, 5.","DOI":"10.20944\/preprints201705.0135.v1"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1248\/bpb.27.1321","article-title":"Antimicrobial Activity of Saturated Fatty Acids and Fatty Amines against Methicillin-Resistant Staphylococcus aureus","volume":"27","author":"Kitahara","year":"2004","journal-title":"Biol. Pharm. Bull."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/j.foodchem.2012.09.057","article-title":"Production of medium chain saturated fatty acids with enhanced antimicrobial activity from crude coconut fat by solid state cultivation of Yarrowia lipolytica","volume":"136","author":"Parfene","year":"2013","journal-title":"Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Togashi, N., Shiraishi, A., Nishizaka, M., Matsuoka, K., Endo, K., Hamashima, H., and Inoue, Y. (2007). Antibacterial activity of long-chain fatty alcohols against Staphylococcus aureus. Molecules, 12.","DOI":"10.3390\/12020139"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2447","DOI":"10.1021\/jf00036a045","article-title":"Antibacterial Activity of Long-Chain Alcohols against Streptococcus mutans","volume":"41","author":"Kubo","year":"1993","journal-title":"J. Agric. Food Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/1574-6968.12043","article-title":"Antibacterial activity of long-chain fatty alcohols against mycobacteria","volume":"338","author":"Mukherjee","year":"2013","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1080\/10942912.2016.1206126","article-title":"Phytochemical composition and in vitro analysis of nopal (O. Ficus-Indica) cladodes at different stages of maturity","volume":"21","year":"2018","journal-title":"Int. J. Food Prop."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"283","DOI":"10.17660\/ActaHortic.2002.581.33","article-title":"Chemical Characterization of Opuntia ficus-indica Seed Oil","volume":"581","author":"Salvo","year":"2002","journal-title":"Acta Hortic."},{"key":"ref_51","first-page":"18","article-title":"Phytochemical screening, antimicrobial, antioxidant, anticancer activities and nutritional values of cactus (Opuntia ficus-indica) pulp and peel","volume":"28","author":"Mohamed","year":"2019","journal-title":"Fresenius Environ. Bull."},{"key":"ref_52","first-page":"1568720","article-title":"Effectiveness of Opuntia ficus indica L. inermis Seed Oil in the Protection and the Healing of Experimentally Induced Gastric Mucosa Ulcer","volume":"2019","author":"Bitri","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1139\/y62-157","article-title":"Hypocholesterolemic agents derived from sterols of marine algae","volume":"40","author":"Reiner","year":"1962","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/S0025-6196(11)63144-3","article-title":"Efficacy and Safety of Plant Stanols and Sterols in the Management of Blood Cholesterol Levels","volume":"78","author":"Katan","year":"2003","journal-title":"Mayo Clin. Proc."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"182","DOI":"10.4103\/0973-1296.111288","article-title":"Sterols isolated from seeds of Panax ginseng and their antiinflammatory activities","volume":"9","author":"Kim","year":"2013","journal-title":"Pharmacogn. Mag."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/S0031-9422(99)00128-4","article-title":"Antitumor sterols from the mycelia of Cordyceps sinensis","volume":"51","author":"Bok","year":"1999","journal-title":"Phytochemistry"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"128248","DOI":"10.1016\/j.bmcl.2021.128248","article-title":"Antibacterial activity of \u03b2-sitosterol isolated from the leaves of Odontonema strictum (Acanthaceae)","volume":"48","author":"Usuki","year":"2021","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"104345","DOI":"10.1016\/j.bse.2021.104345","article-title":"Antibacterial and antioxidant activities of compounds isolated from the leaves of Symphonia globulifera (Clusiaceae) and their chemophenetic significance","volume":"99","author":"Tih","year":"2021","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/j.1365-2621.2008.01744.x","article-title":"Effectiveness of fatty acids and their monoglycerides against gram-negative pathogens","volume":"44","author":"Altieri","year":"2009","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.4315\/0362-028X-70.5.1206","article-title":"Inhibition of Aspergillus spp. and Penicillium spp. by Fatty Acids and Their Monoglycerides","volume":"70","author":"Altieri","year":"2007","journal-title":"J. Food Prot."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1111\/j.1365-2621.2007.01639.x","article-title":"Antifungal activity of fatty acids and their monoglycerides against Fusarium spp. in a laboratory medium","volume":"44","author":"Altieri","year":"2009","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1080\/1071576021000038531","article-title":"Oxidative Stress, Inflammation, and Diabetic Vasculopathies: The Role of Alpha Tocopherol Therapy","volume":"36","author":"Jialal","year":"2002","journal-title":"Free Radic. Res."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Palmeri, R., Parafati, L., Arena, E., Grassenio, E., Restuccia, C., and Fallico, B. (2020). Antioxidant and Antimicrobial Properties of Semi-Processed Frozen Prickly Pear Juice as Affected by Cultivar and Harvest Time. Foods, 9.","DOI":"10.3390\/foods9020235"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"3663315","DOI":"10.1155\/2021\/3663315","article-title":"Review on the Antibacterial Mechanism of Plant-Derived Compounds against Multidrug-Resistant Bacteria (MDR)","volume":"2021","author":"Jubair","year":"2021","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_65","first-page":"74","article-title":"Antibacterial activity of Opuntia ficus-indica skin fruit extracts","volume":"10","author":"Welegerima","year":"2017","journal-title":"Biotechnol. Int."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.foodcont.2005.08.010","article-title":"Antibacterial effect of phenolic compounds from different wines","volume":"2","author":"Vaquero","year":"2007","journal-title":"Food Control"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.lwt.2019.05.028","article-title":"Phenolic compound profile and biological activities of Southern African Opuntia ficus-indica fruit pulp and peels","volume":"111","author":"Aruwa","year":"2019","journal-title":"LWT"},{"key":"ref_68","first-page":"481","article-title":"Opuntia ficus indica tohum ya\u011f fraksiyonlar\u0131n\u0131n antioksidan ve antibakteriyel aktiviteleri ve biyoaktif bile\u015fikleri tan\u0131mlama","volume":"42","author":"Chkioua","year":"2017","journal-title":"Turk. J. Biochem."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1089\/jmf.2013.0089","article-title":"Chemical composition and antibacterial activity of Opuntia ficus-indica f. inermis (cactus pear) flowers","volume":"17","author":"Ennouri","year":"2014","journal-title":"J. Med. Food"},{"key":"ref_70","first-page":"243","article-title":"Phytochemical composition and antibacterial activity of Opuntia ficus indica cladodes extracts","volume":"6","author":"Welegerima","year":"2018","journal-title":"J. Med. Plants Stud."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.13005\/bpj\/1764","article-title":"Antimicrobial, antioxidant, total phenols and flavonoids content of four cactus (opuntiaficus-indica) cultivars","volume":"12","author":"Bargougui","year":"2019","journal-title":"Biomed. Pharmacol. J."},{"key":"ref_72","first-page":"9315360","article-title":"Optimization of ultrasound extraction of cactus pear (Opuntia ficus indica) seed oil based on antioxidant activity and evaluation of its antimicrobial activity","volume":"2017","year":"2017","journal-title":"J. Food Qual."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Mabotja, M.B., Venter, S.L., Du Plooy, C.P., Kudanga, T., and Amoo, S.O. (2021). Phytochemical Content, Antioxidant, Alpha-Glucosidase Inhibitory and Antibacterial Activities of Spineless Cactus Pear Cultivars. Plants, 10.","DOI":"10.3390\/plants10071312"},{"key":"ref_74","first-page":"35","article-title":"Characterization of Opuntia ficus-indica (L.) Mill. fruit volatiles and antibacterial evaluation","volume":"5","author":"Demirci","year":"2018","journal-title":"Nat. Volatiles Essent. Oils"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Blando, F., Russo, R., Negro, C., De Bellis, L., and Frassinetti, S. (2019). Antimicrobial and Antibiofilm Activity against Staphylococcus aureus of Opuntia ficus-indica (L.) Mill. Cladode Polyphenolic Extracts. Antioxidants, 8.","DOI":"10.3390\/antiox8050117"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"809","DOI":"10.21577\/1984-6835.20200063","article-title":"Chemical constituents and antimicrobial activity of branches and leaves of Cordia insignis (Boraginaceae)","volume":"12","author":"Correia","year":"2020","journal-title":"Rev. Virtual Quim."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"8992","DOI":"10.1002\/slct.202001756","article-title":"A New Aliphatic Ketone, Chemical Composition, Antibacterial, Antioxidant and In Vitro Cytotoxic Activities of Lepidium latifolium","volume":"5","author":"Feng","year":"2020","journal-title":"ChemistrySelect"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"302","DOI":"10.3906\/vet-1510-24","article-title":"An investigation of antibacterial effects of steroids","volume":"41","author":"Otlu","year":"2017","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"5257","DOI":"10.1039\/D0FO00769B","article-title":"Unveiling the bioactivity potential of Allium triquetrum L. lipophilic fraction: Chemical characterization and in vitro antibacterial activity against methicillin-resistant staphylococcus aureus","volume":"11","author":"Rabah","year":"2020","journal-title":"Food Funct."},{"key":"ref_80","unstructured":"Helrich, K. (1990). Official Methods of Analysis, Association of Official Analytical Chemists. [15th ed.]."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.indcrop.2019.02.028","article-title":"Valorisation of bark lipophilic fractions from three Portuguese Salix species: A systematic study of the chemical composition and inhibitory activity on Escherichia coli","volume":"132","author":"Ramos","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1016\/j.foodres.2013.09.017","article-title":"The ripe pulp of Mangifera indica L.: A rich source of phytosterols and other lipophilic phytochemicals","volume":"54","author":"Vilela","year":"2013","journal-title":"Food Res. Int."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.foodchem.2014.04.050","article-title":"Lipophilic phytochemicals from banana fruits of several Musa species","volume":"162","author":"Vilela","year":"2014","journal-title":"Food Chem."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Oliveira, C.S.D., Moreira, P., Resende, J., Cruz, M.T., Pereira, C.M.F., Silva, A.M.S., Santos, S.A.O., and Silvestre, A.J.D. (2020). Characterization and cytotoxicity assessment of the lipophilic fractions of different morphological parts of acacia dealbata. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21051814"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1007\/s10811-016-0855-y","article-title":"Bioprospecting for lipophilic-like components of five Phaeophyta macroalgae from the Portuguese coast","volume":"28","author":"Santos","year":"2016","journal-title":"J. Appl. Phycol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Santos, S.A.O., Trindade, S.S., Oliveira, C.S.D., Parreira, P., Rosa, D., Duarte, M.F., Ferreira, I., Cruz, M.T., Rego, A.M., and Abreu, M.H. (2017). Lipophilic fraction of cultivated Bifurcaria bifurcata R. Ross: Detailed composition and in vitro prospection of current challenging bioactive properties. Mar. Drugs, 15.","DOI":"10.3390\/md15110340"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Salvador, \u00c2.C., Sim\u00f5es, M.M.Q., Silva, A.M.S., Santos, S.A.O., Rocha, S.M., and Silvestre, A.J.D. (2019). Vine waste valorisation: Integrated approach for the prospection of bioactive lipophilic phytochemicals. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20174239"},{"key":"ref_88","unstructured":"SFM (2019). Recommandations 2019 V.1.0 Janvier. Clin. Microbiol. Infect., 2, 144."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1016\/j.foodcont.2010.12.015","article-title":"Antimicrobial activity of Pistacia lentiscus and Satureja montana essential oils against Listeria monocytogenes CECT 935 using laboratory media: Efficacy and synergistic potential in minced beef","volume":"22","author":"Djenane","year":"2011","journal-title":"Food Control"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/19\/11161\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:37:29Z","timestamp":1760143049000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/19\/11161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,22]]},"references-count":89,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["ijms231911161"],"URL":"https:\/\/doi.org\/10.3390\/ijms231911161","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,22]]}}}