{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:00:39Z","timestamp":1772823639092,"version":"3.50.1"},"reference-count":34,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T00:00:00Z","timestamp":1664236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Aveiro, CICECO-Aveiro Institute of Materials","award":["UIDB\/5011\/2020"],"award-info":[{"award-number":["UIDB\/5011\/2020"]}]},{"name":"University of Aveiro, CICECO-Aveiro Institute of Materials","award":["UIDP\/50011\/2020"],"award-info":[{"award-number":["UIDP\/50011\/2020"]}]},{"name":"national funds","award":["UIDB\/5011\/2020"],"award-info":[{"award-number":["UIDB\/5011\/2020"]}]},{"name":"national funds","award":["UIDP\/50011\/2020"],"award-info":[{"award-number":["UIDP\/50011\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This work presents the first evidence that dissolved globular proteins change the arrangement of hydrogen bonds in water, with different proteins showing quantitatively different effects. Using ATR-FTIR (attenuated total reflection\u2014Fourier transform infrared) spectroscopic analysis of OH-stretch bands, we obtain quantitative estimates of the relative amounts of the previously reported four subpopulations of water structures coexisting in a variety of aqueous solutions. Where solvatochromic dyes can measure the properties of solutions of non-ionic polymers, the results correlate well with ATR-FTIR measurements. In protein solutions to which solvatochromic dye probes cannot be applied, NMR (nuclear magnetic resonance) spectroscopy was used for the first time to estimate the hydrogen bond donor acidity of water. We found strong correlations between the solvent acidity and arrangement of hydrogen bonds in aqueous solutions for several globular proteins. Even quite similar proteins are found to change water properties in dramatically different ways.<\/jats:p>","DOI":"10.3390\/ijms231911381","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T23:12:12Z","timestamp":1664320332000},"page":"11381","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Arrangement of Hydrogen Bonds in Aqueous Solutions of Different Globular Proteins"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9670-2634","authenticated-orcid":false,"given":"Amber R.","family":"Titus","sequence":"first","affiliation":[{"name":"Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH 44114, USA"}]},{"given":"Pedro P.","family":"Madeira","sequence":"additional","affiliation":[{"name":"Centro de Investigacao em Materiais Ceramicos e Compositos, Department of Chemistry, 3810-193 Aveiro, Portugal"}]},{"given":"Luisa A.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH 44114, USA"}]},{"given":"Alexander I.","family":"Belgovskiy","sequence":"additional","affiliation":[{"name":"Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH 44114, USA"}]},{"given":"Elizabeth K.","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Physics, Kent State University, Kent, OH 44242, USA"}]},{"suffix":"Jr.","given":"Jay Adin","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Case Western Reserve University, Cleveland, OH 44242, USA"}]},{"given":"William V.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Scattering Solutions, Inc., Cleveland, OH 44242, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2721-5995","authenticated-orcid":false,"given":"Anthony E.","family":"Smart","sequence":"additional","affiliation":[{"name":"Scattering Solutions, Inc., Costa Mesa, CA 02138, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4037-5857","authenticated-orcid":false,"given":"Vladimir N.","family":"Uversky","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Byrd Alzheimer\u2019s Research Institute, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd., Tampa, FL 33612, USA"}]},{"given":"Boris Y.","family":"Zaslavsky","sequence":"additional","affiliation":[{"name":"Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH 44114, USA"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1021\/cr068037a","article-title":"Water as an active constituent in cell biology","volume":"108","author":"Ball","year":"2008","journal-title":"Chem. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"13327","DOI":"10.1073\/pnas.1703781114","article-title":"Water is an active matrix of life for cell and molecular biology","volume":"114","author":"Ball","year":"2017","journal-title":"Proc. Natl. Acad. Sci. 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