{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T11:48:43Z","timestamp":1775216923211,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,5]],"date-time":"2022-11-05T00:00:00Z","timestamp":1667606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00051\/2020"],"award-info":[{"award-number":["UIDB\/00051\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/00051\/2020"],"award-info":[{"award-number":["UIDP\/00051\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04501\/2020"],"award-info":[{"award-number":["UIDB\/04501\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-007628"],"award-info":[{"award-number":["POCI-01-0145-FEDER-007628"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["2022.10080.BD"],"award-info":[{"award-number":["2022.10080.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000003"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000003"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["823839"],"award-info":[{"award-number":["823839"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["UIDB\/00051\/2020"],"award-info":[{"award-number":["UIDB\/00051\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["UIDP\/00051\/2020"],"award-info":[{"award-number":["UIDP\/00051\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["UIDB\/04501\/2020"],"award-info":[{"award-number":["UIDB\/04501\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-007628"],"award-info":[{"award-number":["POCI-01-0145-FEDER-007628"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["2022.10080.BD"],"award-info":[{"award-number":["2022.10080.BD"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000003"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000003"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"publisher","award":["823839"],"award-info":[{"award-number":["823839"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["UIDB\/00051\/2020"],"award-info":[{"award-number":["UIDB\/00051\/2020"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["UIDP\/00051\/2020"],"award-info":[{"award-number":["UIDP\/00051\/2020"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["UIDB\/04501\/2020"],"award-info":[{"award-number":["UIDB\/04501\/2020"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-007628"],"award-info":[{"award-number":["POCI-01-0145-FEDER-007628"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["2022.10080.BD"],"award-info":[{"award-number":["2022.10080.BD"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000003"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000003"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017790","name":"EPIC-XS","doi-asserted-by":"publisher","award":["823839"],"award-info":[{"award-number":["823839"]}],"id":[{"id":"10.13039\/100017790","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Coronary artery disease (CAD) and the frequently coexisting aortic valve stenosis (AVS) are heart diseases accounting for most cardiac surgeries. These share many risk factors, such as age, diabetes, hypertension, or obesity, and similar pathogenesis, including endothelial disruption, lipid and immune cell infiltration, inflammation, fibrosis, and calcification. Unsuspected CAD and AVS are sometimes detected opportunistically through echocardiography, coronary angiography, and magnetic resonance. Routine biomarkers for early detection of either of these atherosclerotic-rooted conditions would be important to anticipate the diagnosis. With a noninvasive collection, urine is appealing for biomarker assessment. We conducted a shotgun proteomics exploratory analysis of urine from 12 CAD and\/or AVS patients and 11 controls to identify putative candidates to differentiate these diseases from healthy subjects. Among the top 20 most dysregulated proteins, TIMP1, MMP2 and vWF stood out, being at least 2.5\u00d7 increased in patients with CAD\/AVS and holding a central position in a network of protein-protein interactions. Moreover, their assessment in an independent cohort (19 CAD\/AVS and 10 controls) evidenced strong correlations between urinary TIMP1 and vWF levels and a common cardiovascular risk factor - HDL (r = 0.59, p &lt; 0.05, and r = 0.64, p &lt; 0.01, respectively).<\/jats:p>","DOI":"10.3390\/ijms232113579","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T11:56:36Z","timestamp":1667908596000},"page":"13579","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Coronary Artery Disease and Aortic Valve Stenosis: A Urine Proteomics Study"],"prefix":"10.3390","volume":"23","author":[{"given":"Lu\u00eds","family":"Perp\u00e9tuo","sequence":"first","affiliation":[{"name":"iBiMED\u2014Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal"},{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9103-5852","authenticated-orcid":false,"given":"Ant\u00f3nio S.","family":"Barros","sequence":"additional","affiliation":[{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"given":"J\u00e9ssica","family":"Dalsuco","sequence":"additional","affiliation":[{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"given":"Rita","family":"Nogueira-Ferreira","sequence":"additional","affiliation":[{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"given":"Pedro","family":"Resende-Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"given":"In\u00eas","family":"Falc\u00e3o-Pires","sequence":"additional","affiliation":[{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6872-4051","authenticated-orcid":false,"given":"Rita","family":"Ferreira","sequence":"additional","affiliation":[{"name":"LAQV\/REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7808-3596","authenticated-orcid":false,"given":"Adelino","family":"Leite-Moreira","sequence":"additional","affiliation":[{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3415-2040","authenticated-orcid":false,"given":"F\u00e1bio","family":"Trindade","sequence":"additional","affiliation":[{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3636-5805","authenticated-orcid":false,"given":"Rui","family":"Vitorino","sequence":"additional","affiliation":[{"name":"iBiMED\u2014Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal"},{"name":"Cardiovascular R&D Centre\u2014UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine of the University of Porto, 4200-319 Porto, Portugal"},{"name":"LAQV\/REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,5]]},"reference":[{"key":"ref_1","unstructured":"Mendis, S., Puska, P., and Norrving, B. (2011). Global Atlas on Cardiovascular Disease Prevention and Control, World Stroke Organ."},{"key":"ref_2","unstructured":"Brown, J.C., Gerhardt, T.E., and Kwon, E. (2022). Risk Factors for Coronary Artery Disease, StatPearls Publishing."},{"key":"ref_3","unstructured":"GBD 2017 Causes of Death Collaborators (2018). Global, Regional, and National Age-Sex-Specific Mortality for 282 Causes of Death in 195 Countries and Territories, 1980\u20132017: A Systematic Analysis for the Global Burden of Disease Study 2017. Lancet, 392, 1736."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e005593","DOI":"10.1161\/JAHA.117.005593","article-title":"Clinical Conundrum of Coronary Artery Disease and Aortic Valve Stenosis","volume":"6","author":"Nishimura","year":"2017","journal-title":"J. Am. Heart Assoc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8","DOI":"10.12703\/P7-08","article-title":"Pathophysiology of Coronary Artery Disease Leading to Acute Coronary Syndromes","volume":"7","author":"Ambrose","year":"2015","journal-title":"F1000Prime Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pop.2012.12.001","article-title":"Coronary Artery Disease","volume":"40","author":"Hanson","year":"2013","journal-title":"Prim. Care"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.1161\/01.CIR.103.11.1522","article-title":"Bone Formation and Inflammation in Cardiac Valves","volume":"103","author":"Mohler","year":"2001","journal-title":"Circulation"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1854","DOI":"10.1016\/j.jacc.2012.02.093","article-title":"Calcific Aortic Stenosis: A Disease of the Valve and the Myocardium","volume":"60","author":"Dweck","year":"2012","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/0002-9343(91)90050-8","article-title":"Update on the Pathogenesis of Atherosclerosis","volume":"91","author":"Wissler","year":"1991","journal-title":"Am. J. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"16006","DOI":"10.1038\/nrdp.2016.6","article-title":"Calcific Aortic Stenosis","volume":"2","author":"Lindman","year":"2016","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1161\/01.ATV.16.4.523","article-title":"Apolipoproteins B, (a), and E Accumulate in the Morphologically Early Lesion of \u201cdegenerative\u201d Valvular Aortic Stenosis","volume":"16","author":"Reichenbach","year":"1996","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_12","unstructured":"Regmi, M., and Siccardi, M.A. (2021). Coronary Artery Disease Prevention. [Updated 2021 Aug 11], StatPearls Publishing."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"820","DOI":"10.7556\/jaoa.2013.057","article-title":"Aortic Stenosis: New Thoughts on a Cardiac Disease of Older People","volume":"113","author":"Rogers","year":"2013","journal-title":"J. Osteopath. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1056\/NEJM199901143400207","article-title":"Atherosclerosis\u2014An Inflammatory Disease","volume":"340","author":"Ross","year":"1999","journal-title":"N. Engl. J. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1136\/bmj.326.7397.1027","article-title":"Pathophysiology and Investigation of Coronary Artery Disease","volume":"326","author":"Grech","year":"2003","journal-title":"BMJ"},{"key":"ref_16","first-page":"185","article-title":"Pathogenesis of Aortic Stenosis: Not Just a Matter of Wear and Tear","volume":"1","author":"Sverdlov","year":"2011","journal-title":"Am. J. Cardiovasc. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1016\/j.jacc.2018.08.2147","article-title":"Macrophage Trafficking, Inflammatory Resolution, and Genomics in Atherosclerosis: JACC Macrophage in CVD Series (Part 2)","volume":"72","author":"Moore","year":"2018","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1161\/CIRCULATIONAHA.110.989152","article-title":"Asymptomatic Severe Aortic Stenosis: Silence of the Lambs?","volume":"122","author":"Shah","year":"2010","journal-title":"Circulation"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1097\/HCO.0b013e3283315553","article-title":"The Gold Standard for Noninvasive Imaging in Coronary Heart Disease: Magnetic Resonance Imaging","volume":"24","author":"Vick","year":"2009","journal-title":"Curr. Opin. Cardiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1161\/CIRCULATIONAHA.113.002310","article-title":"Accurate Assessment of Aortic Stenosis","volume":"129","author":"Saikrishnan","year":"2014","journal-title":"Circulation"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.4065\/mcp.2009.0391","article-title":"Chronic Coronary Artery Disease: Diagnosis and Management","volume":"84","author":"Cassar","year":"2009","journal-title":"Mayo Clin. Proc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.amjmed.2016.10.005","article-title":"Aortic Stenosis: Pathophysiology, Diagnosis, and Therapy","volume":"130","author":"Joseph","year":"2017","journal-title":"Am. J. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"456","DOI":"10.2174\/1389201018666170630120805","article-title":"Biomarkers in Stable Coronary Artery Disease","volume":"18","author":"Rusnak","year":"2017","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.ahj.2017.10.016","article-title":"Biomarkers in Stable Coronary Artery Disease","volume":"196","author":"McCarthy","year":"2018","journal-title":"Am. Heart J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1080\/24748706.2017.1329959","article-title":"Biomarkers in Aortic Stenosis: A Systematic Review","volume":"1","author":"Redfors","year":"2017","journal-title":"Struct. Heart"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1093\/eurheartj\/ehaa575","article-title":"2020 ESC Guidelines for the Management of Acute Coronary Syndromes in Patients Presenting without Persistent ST-Segment Elevation","volume":"42","author":"Collet","year":"2021","journal-title":"Eur. Heart J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1093\/eurheartj\/ehz425","article-title":"2019 ESC Guidelines for the Diagnosis and Management of Chronic Coronary Syndromes","volume":"41","author":"Knuuti","year":"2020","journal-title":"Eur. Heart J."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"van Holten, T.C., Waanders, L.F., de Groot, P.G., Vissers, J., Hoefer, I.E., Pasterkamp, G., Prins, M.W.J., and Roest, M. (2013). Circulating Biomarkers for Predicting Cardiovascular Disease Risk; a Systematic Review and Comprehensive Overview of Meta-Analyses. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0062080"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1093\/eurheartj\/ehab395","article-title":"2021 ESC\/EACTS Guidelines for the Management of Valvular Heart Disease","volume":"43","author":"Vahanian","year":"2022","journal-title":"Eur. Heart J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep28042","article-title":"Plasma Proteomic Analysis of Stable Coronary Artery Disease Indicates Impairment of Reverse Cholesterol Pathway","volume":"6","author":"Basak","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"790289","DOI":"10.3389\/fcvm.2021.790289","article-title":"Differential Proteomics of Cardiovascular Risk and Coronary Artery Disease in Humans","volume":"8","author":"Ferrannini","year":"2021","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1161\/CIRCULATIONAHA.117.030414","article-title":"Proteomic Biomarkers for Incident Aortic Stenosis Requiring Valvular Replacement","volume":"138","author":"Ljungberg","year":"2018","journal-title":"Circulation"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.jprot.2009.07.001","article-title":"The Dynamic Range Problem in the Analysis of the Plasma Proteome","volume":"73","author":"Hortin","year":"2010","journal-title":"J. Proteomics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1074\/mcp.R800001-MCP200","article-title":"Urine in Clinical Proteomics","volume":"7","author":"Decramer","year":"2008","journal-title":"Mol. Cell. Proteom."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/dia.2008.0040","article-title":"Evaluation of Urinary Biomarkers for Coronary Artery Disease, Diabetes, and Diabetic Kidney Disease","volume":"11","author":"Maahs","year":"2009","journal-title":"Diabetes Technol. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e0589","DOI":"10.1097\/MD.0000000000010589","article-title":"Impact of von Willebrand Factor on Coronary Plaque Burden in Coronary Artery Disease Patients Treated with Statins","volume":"97","author":"Kato","year":"2018","journal-title":"Medicine"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Fan, M., Wang, X., Peng, X., Feng, S., Zhao, J., Liao, L., Zhang, Y., Hou, Y., and Liu, J. (2020). Prognostic Value of Plasma von Willebrand Factor Levels in Major Adverse Cardiovascular Events: A Systematic Review and Meta-Analysis. BMC Cardiovasc. Disord., 20.","DOI":"10.1186\/s12872-020-01375-7"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1076029619900552","DOI":"10.1177\/1076029619900552","article-title":"Elevated Plasma von Willebrand Factor Antigen and Activity Levels Are Associated With the Severity of Coronary Stenosis","volume":"26","author":"Yan","year":"2020","journal-title":"Clin. Appl. Thromb."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1056\/NEJMoa1505643","article-title":"Von Willebrand Factor Multimers during Transcatheter Aortic-Valve Replacement","volume":"375","author":"Rauch","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1007\/s00392-004-1021-0","article-title":"Expression and Activity of Matrix Metalloproteinase-2 in Calcific Aortic Stenosis","volume":"93","author":"Kaden","year":"2004","journal-title":"Z. Kardiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1161\/res.90.3.251","article-title":"Matrix Metalloproteinases in Vascular Remodeling and Atherogenesis","volume":"90","author":"Galis","year":"2002","journal-title":"Circ. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0021-9150(03)00251-X","article-title":"Different Expression of MMPs\/TIMP-1 in Human Atherosclerotic Lesions. Relation to Plaque Features and Vascular Bed","volume":"170","author":"Orbe","year":"2003","journal-title":"Atherosclerosis"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.atherosclerosis.2006.07.027","article-title":"Urinary Levels of Matrix Metalloproteinase 9 and 2 and Tissue Inhibitor of Matrix Metalloproteinase in Patients with Coronary Artery Disease","volume":"194","author":"Fitzsimmons","year":"2007","journal-title":"Atherosclerosis"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1016\/j.ehj.2004.05.029","article-title":"Relations of Plasma Total TIMP-1 Levels to Cardiovascular Risk Factors and Echocardiographic Measures: The Framingham Heart Study","volume":"25","author":"Evans","year":"2004","journal-title":"Eur. Heart J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1182\/blood-2014-09-599530","article-title":"High-Density Lipoprotein Modulates Thrombosis by Preventing von Willebrand Factor Self-Association and Subsequent Platelet Adhesion","volume":"127","author":"Chung","year":"2016","journal-title":"Blood"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e9021","DOI":"10.15252\/msb.20199021","article-title":"R2-P2 Rapid-Robotic Phosphoproteomics Enables Multidimensional Cell Signaling Studies","volume":"15","author":"Leutert","year":"2019","journal-title":"Mol. Syst. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"T\u00fcrker, C., Akal, F., Joho, D., Panse, C., Barkow-Oesterreicher, S., Rehrauer, H., and Schlapbach, R. (2010, January 22\u201326). B-Fabric: The Swiss Army Knife for Life Sciences. Proceedings of the EDBT \u201910: 13th International Conference on Extending Database Technology, Lausanne, Switzerland.","DOI":"10.1145\/1739041.1739135"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant Enables High Peptide Identification Rates, Individualized p.p.b.-Range Mass Accuracies and Proteome-Wide Protein Quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"ref_49","unstructured":"Wolski, C.P.W., and Grossmann, J. (2022, March 01). SRMService\u2014R-Package to Report Quantitative Mass Spectrometry Data. Available online: https:\/\/github.com\/protViz\/SRMService."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/21\/13579\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:11:18Z","timestamp":1760145078000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/21\/13579"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,5]]},"references-count":49,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ijms232113579"],"URL":"https:\/\/doi.org\/10.3390\/ijms232113579","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,5]]}}}