{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T19:55:18Z","timestamp":1778010918155,"version":"3.51.4"},"reference-count":58,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,13]],"date-time":"2022-11-13T00:00:00Z","timestamp":1668297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["UIDB\/04469\/2020"],"award-info":[{"award-number":["UIDB\/04469\/2020"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["LA\/P\/0029\/2020"],"award-info":[{"award-number":["LA\/P\/0029\/2020"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["2021.02803.CEECIND"],"award-info":[{"award-number":["2021.02803.CEECIND"]}]},{"name":"Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems","award":["UIDB\/04469\/2020"],"award-info":[{"award-number":["UIDB\/04469\/2020"]}]},{"name":"Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems","award":["LA\/P\/0029\/2020"],"award-info":[{"award-number":["LA\/P\/0029\/2020"]}]},{"name":"Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems","award":["2021.02803.CEECIND"],"award-info":[{"award-number":["2021.02803.CEECIND"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["UIDB\/04469\/2020"],"award-info":[{"award-number":["UIDB\/04469\/2020"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["LA\/P\/0029\/2020"],"award-info":[{"award-number":["LA\/P\/0029\/2020"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["2021.02803.CEECIND"],"award-info":[{"award-number":["2021.02803.CEECIND"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Onychomycosis is the most common nail fungal infection worldwide. There are several therapy options available for onychomycosis, such as oral antifungals, topicals, and physical treatments. Terbinafine is in the frontline for the treatment of onychomycosis; however, several adverse effects are associated to its oral administration. In this work, innovative keratin-based carriers encapsulating terbinafine were designed to overcome the drawbacks related to the use this drug. Therapeutic textiles functionalized with keratin-based particles (100% keratin; 80% keratin\/20% keratin-PEG) encapsulating terbinafine were developed. The controlled release of terbinafine from the functionalized textiles was evaluated against different mimetic biologic solutions (PBS buffer\u2014pH = 7.4, micellar solution and acidic sweat solution\u2014pH = 4.3). The modification of keratin with polyethylene glycol (PEG) moieties favored the release of terbinafine at the end of 48 h for all the solution conditions. When the activity of functionalized textiles was tested against Trichophyton rubrum, a differentiated inhibition was observed. Textiles functionalized with 80% keratin\/20% keratin-PEG encapsulating terbinafine showed a 2-fold inhibition halo compared with the textiles containing 100% keratin-encapsulating terbinafine. No activity was observed for the textiles functionalized with keratin-based particles without terbinafine. The systems herein developed revealed therapeutic potential towards nail fungal infections, taking advantage of keratin-based particles affinity to keratin structures and of the keratinase activity of T. rubrum.<\/jats:p>","DOI":"10.3390\/ijms232213999","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T02:29:32Z","timestamp":1668392972000},"page":"13999","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Therapeutic Textiles Functionalized with Keratin-Based Particles Encapsulating Terbinafine for the Treatment of Onychomycosis"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0409-3940","authenticated-orcid":false,"given":"Andr\u00e9 F.","family":"Costa","sequence":"first","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2103-0482","authenticated-orcid":false,"given":"Salom\u00e9","family":"Lu\u00eds","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5480-937X","authenticated-orcid":false,"given":"Jennifer","family":"Noro","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0000-7387","authenticated-orcid":false,"given":"S\u00f3nia","family":"Silva","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"},{"name":"INIAV\u2014National Institute for Agrarian and Veterinarian Research, Rua dos Lagidos, 4485-655 Vair\u00e3o, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6252-8693","authenticated-orcid":false,"given":"Carla","family":"Silva","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3298-5564","authenticated-orcid":false,"given":"Artur","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.jaad.2018.05.1260","article-title":"Onychomycosis","volume":"80","author":"Lipner","year":"2019","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_2","first-page":"Online, PMID: 30215902","article-title":"Terbinafine for Onychomycosis","volume":"98","author":"Hawks","year":"2018","journal-title":"Am. Fam. Physician"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1111\/jdv.16394","article-title":"Onychomycosis: A Review","volume":"34","author":"Gupta","year":"2020","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zhang, M., Jiang, L., Li, F., Xu, Y., Lv, S., and Wang, B. (2019). Simultaneous Dermatophytosis and Keratomycosis Caused by Trichophyton Interdigitale Infection: A Case Report and Literature Review. BMC Infect. Dis., 19.","DOI":"10.1186\/s12879-019-4612-0"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s40266-021-00917-8","article-title":"Onychomycosis in Older Adults: Prevalence, Diagnosis, and Management","volume":"39","author":"Gupta","year":"2022","journal-title":"Drugs Aging"},{"key":"ref_6","unstructured":"Bodman, M.A., and Krishnamurthy, K. (2022). Onychomycosis. StatPearls [Internet], StatPearls Publishing."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3","DOI":"10.7547\/17-201","article-title":"Trichophyton Rubrum DNA Strains in Patients with Onychomycosis with Persistent Mixed Infections Involving a Nondermatophyte Mold","volume":"110","author":"Gupta","year":"2020","journal-title":"J. Am. Podiatr. Med. Assoc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3959","DOI":"10.1038\/s41598-021-83632-z","article-title":"Metabolomic Analysis of Trichophyton Rubrum and Microsporum Canis during Keratin Degradation","volume":"11","author":"Ciesielska","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_9","first-page":"000707","article-title":"The Taxonomy of the Trichophyton Rubrum Complex: A Phylogenomic Approach","volume":"7","author":"Cornet","year":"2021","journal-title":"Microb. Genom."},{"key":"ref_10","first-page":"762","article-title":"Onychomycosis: Current Trends in Diagnosis and Treatment","volume":"88","author":"Westerberg","year":"2013","journal-title":"Am. Fam. Physician"},{"key":"ref_11","first-page":"32","article-title":"Onychomycosis: An Updated Review","volume":"14","author":"Leung","year":"2020","journal-title":"Recent Pat. Inflamm. Allergy Drug Discov."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100949","DOI":"10.1016\/j.mycmed.2020.100949","article-title":"Treatment and Management Strategies of Onychomycosis","volume":"30","author":"Aggarwal","year":"2020","journal-title":"J. Mycol. Med."},{"key":"ref_13","first-page":"203","article-title":"Transungual Drug Delivery: An Overview","volume":"2","author":"Rajendra","year":"2012","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1111\/ics.12142","article-title":"Transungual Delivery: Deliberations and Creeds","volume":"36","author":"Thatai","year":"2014","journal-title":"Int. J. Cosmet. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1365-2133.1992.tb00001.x","article-title":"Terbinafine: Mode of Action and Properties of the Squalene Epoxidase Inhibition","volume":"126","author":"Ryder","year":"1992","journal-title":"Br. J. Dermatol."},{"key":"ref_16","unstructured":"Maxfield, L., Preuss, C.V., and Bermudez, R. (2022). Terbinafine. StatPearls [Internet], StatPearls Publishing."},{"key":"ref_17","first-page":"CD010031","article-title":"Oral Antifungal Medication for Toenail Onychomycosis","volume":"7","author":"Hawke","year":"2017","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1039\/C7BM00411G","article-title":"Keratin: Dissolution, Extraction and Biomedical Application","volume":"5","author":"Shavandi","year":"2017","journal-title":"Biomater. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1111\/ics.12483","article-title":"Keratin-Based Particles for Protection and Restoration of Hair Properties","volume":"40","author":"Tinoco","year":"2018","journal-title":"Int. J. Cosmet. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.msec.2018.05.021","article-title":"Transparent Biocompatible Wool Keratin Film Prepared by Mechanical Compression of Porous Keratin Hydrogel","volume":"91","author":"Mori","year":"2018","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.ejpb.2010.11.023","article-title":"Manufacture and Characterization of Mucoadhesive Buccal Films","volume":"77","author":"Morales","year":"2011","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/j.ijbiomac.2020.02.122","article-title":"Ohmic Heating as an Innovative Approach for the Production of Keratin Films","volume":"150","author":"Tinoco","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0168-1656(01)00395-9","article-title":"Fabrication of Wool Keratin Sponge Scaffolds for Long-Term Cell Cultivation","volume":"93","author":"Tachibana","year":"2002","journal-title":"J. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.msec.2017.05.076","article-title":"Novel Bilayer Wound Dressing Based on Electrospun Gelatin\/Keratin Nanofibrous Mats for Skin Wound Repair","volume":"79","author":"Yao","year":"2017","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.msec.2017.02.148","article-title":"Dual-Layered 3D Nanofibrous Matrix Incorporated with Dual Drugs and Their Synergetic Effect on Accelerating Wound Healing through Growth Factor Regulation","volume":"76","author":"Singaravelu","year":"2017","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.msec.2018.04.088","article-title":"Organic Solvent-Free Preparation of Keratin Nanoparticles as Doxorubicin Carriers for Antitumour Activity","volume":"90","author":"Aluigi","year":"2018","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.msec.2018.05.073","article-title":"Triple Stimuli-Responsive Keratin Nanoparticles as Carriers for Drug and Potential Nitric Oxide Release","volume":"91","author":"Li","year":"2018","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1900","DOI":"10.1021\/acs.bioconjchem.5b00289","article-title":"Hydrophobically Modified Keratin Vesicles for GSH-Responsive Intracellular Drug Release","volume":"26","author":"Curcio","year":"2015","journal-title":"Bioconjug. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"12109","DOI":"10.1039\/C8NR03265C","article-title":"Tumor-Targeted and Nitric Oxide-Generated Nanogels of Keratin and Hyaluronan for Enhanced Cancer Therapy","volume":"10","author":"Sun","year":"2018","journal-title":"Nanoscale"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.carbpol.2017.07.078","article-title":"Bio-Responsive Alginate-Keratin Composite Nanogels with Enhanced Drug Loading Efficiency for Cancer Therapy","volume":"175","author":"Sun","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.eurpolymj.2018.05.030","article-title":"Keratin-Hydrotalcites Hybrid Films for Drug Delivery Applications","volume":"105","author":"Posati","year":"2018","journal-title":"Eur. Polym. J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.ijbiomac.2020.01.144","article-title":"Methods of Keratin Extraction from Poultry Feathers and Their Effects on Antioxidant Activity of Extracted Keratin","volume":"148","author":"Alahyaribeik","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jconrel.2012.04.036","article-title":"Protein-Based Nanocarriers as Promising Drug and Gene Delivery Systems","volume":"161","author":"Elzoghby","year":"2012","journal-title":"J. Control. Release"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/j.colsurfb.2019.06.057","article-title":"DOX-Conjugated Keratin Nanoparticles for PH-Sensitive Drug Delivery","volume":"181","author":"Liu","year":"2019","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.addr.2015.09.012","article-title":"PEGylation as a Strategy for Improving Nanoparticle-Based Drug and Gene Delivery","volume":"99","author":"Suk","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"10748","DOI":"10.1039\/D1NR02065J","article-title":"Effects of Polyethylene Glycol on the Surface of Nanoparticles for Targeted Drug Delivery","volume":"13","author":"Shi","year":"2021","journal-title":"Nanoscale"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/jmr.2405","article-title":"Aldehyde PEGylation of Laccase from Trametes Versicolor in Route to Increase Its Stability: Effect on Enzymatic Activity","volume":"28","year":"2015","journal-title":"J. Mol. Recognit."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1002\/jps.23887","article-title":"Controlled-Release Injectable Containing Terbinafine\/PLGA Microspheres for Onychomycosis Treatment","volume":"103","author":"Angamuthu","year":"2014","journal-title":"J. Pharm. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"113067","DOI":"10.1016\/j.indcrop.2020.113067","article-title":"Keratin:Zein Particles as Vehicles for Fragrance Release on Hair","volume":"159","author":"Tinoco","year":"2021","journal-title":"Ind. Crops Prod."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3939","DOI":"10.1021\/acsbiomaterials.8b01098","article-title":"Protein Nanoparticles: Promising Platforms for Drug Delivery Applications","volume":"4","author":"Jain","year":"2018","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.msec.2014.10.011","article-title":"Dithranol-Loaded Lipid-Core Nanocapsules Improve the Photostability and Reduce the in Vitro Irritation Potential of This Drug","volume":"46","author":"Savian","year":"2015","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_42","first-page":"1621","article-title":"Preparation and Evaluation of Quercetin-Loaded Lecithin-Chitosan Nanoparticles for Topical Delivery","volume":"6","author":"Tan","year":"2011","journal-title":"Int. J. Nanomed."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12951-018-0353-2","article-title":"Development of Keratin Nanoparticles for Controlled Gastric Mucoadhesion and Drug Release","volume":"16","author":"Cheng","year":"2018","journal-title":"J. Nanobiotech."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"104664","DOI":"10.1016\/j.reactfunctpolym.2020.104664","article-title":"Zein Impart Hydrophobic and Antimicrobial Properties to Cotton Textiles","volume":"154","author":"Torres","year":"2020","journal-title":"React. Funct. Polym."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Rani, S., Sharma, A.K., Khan, I., Gothwal, A., Chaudhary, S., and Gupta, U. (2017). Polymeric Nanoparticles in Targeting and Delivery of Drugs. Nanotechnology-Based Approaches for Targeting and Delivery of Drugs and Genes, Elsevier.","DOI":"10.1016\/B978-0-12-809717-5.00008-7"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.jare.2016.03.003","article-title":"In Vitro Assessment of Pharmaceutical Potential of Ethosomes Entrapped with Terbinafine Hydrochloride","volume":"7","author":"Iizhar","year":"2016","journal-title":"J. Adv. Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Abril, D., Mirabal-Gallardo, Y., Gonz\u00e1lez, A., Marican, A., Dur\u00e1n-Lara, E.F., Silva Santos, L., and Vald\u00e9s, O. (2019). Comparison of the Oxidative Stability and Antioxidant Activity of Extra-Virgin Olive Oil and Oils Extracted from Seeds of Colliguaya Integerrima and Cynara Cardunculus Under Normal Conditions and After Thermal Treatment. Antioxidants, 8.","DOI":"10.3390\/antiox8100470"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"390","DOI":"10.3389\/fbioe.2019.00390","article-title":"Polymeric Electrospun Fibrous Dressings for Topical Co-Delivery of Acyclovir and Omega-3 Fatty Acids","volume":"7","author":"Costa","year":"2019","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1080\/10837450.2019.1667387","article-title":"Terbinafine-Loaded Branched PLGA-Based Cationic Nanoparticles with Modifiable Properties","volume":"24","author":"Martiska","year":"2019","journal-title":"Pharm. Dev. Technol."},{"key":"ref_50","first-page":"18","article-title":"Enhanced Transdermal Permeability of Terbinafine through Novel Nanoemulgel Formulation; Development, In Vitro and In Vivo Characterization","volume":"4","author":"Elmataeeshy","year":"2018","journal-title":"Futur. J. Pharm. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0378-5173(01)00804-3","article-title":"Controlled Release of Antifungal Drug Terbinafine Hydrochloride from Poly(N-Vinyl 2-Pyrrolidone\/Itaconic Acid) Hydrogels","volume":"228","author":"Yakar","year":"2001","journal-title":"Int. J. Pharm."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Mishra, R., Militky, J., and Venkataraman, M. (2019). Nanoporous Materials. Nanotechnology in Textiles, Elsevier.","DOI":"10.1016\/B978-0-08-102609-0.00007-9"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s40691-021-00285-2","article-title":"Comfort Related Woven Fabric Transmission Properties Made of Cotton and Nylon","volume":"9","author":"Adamu","year":"2022","journal-title":"Fash. Text."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"21080","DOI":"10.1038\/s41598-020-78305-2","article-title":"Thermophysiological Comfort of Zinc Oxide Nanoparticles Coated Woven Fabrics","volume":"10","author":"Noman","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Saleemi, S., Naveed, T., Riaz, T., Memon, H., Awan, J.A., Siyal, M.I., Xu, F., and Bae, J. (2020). Surface Functionalization of Cotton and PC Fabrics Using SiO2 and ZnO Nanoparticles for Durable Flame Retardant Properties. Coatings, 10.","DOI":"10.3390\/coatings10020124"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1007\/s10924-015-0725-8","article-title":"Keratin Extracted from Chicken Feather Waste: Extraction, Preparation, and Structural Characterization of the Keratin and Keratin\/Biopolymer Films and Electrospuns","volume":"23","author":"Ayutthaya","year":"2015","journal-title":"J. Polym. Environ."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Tinoco, A., Costa, A.F., Lu\u00eds, S., Martins, M., Cavaco-Paulo, A., and Ribeiro, A. (2021). Proteins as Hair Styling Agents. Appl. Sci., 11.","DOI":"10.3390\/app11094245"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"111784","DOI":"10.1016\/j.msec.2020.111784","article-title":"Ohmic Heating as a New Tool for Protein Scaffold Engineering","volume":"120","author":"Rodrigues","year":"2021","journal-title":"Mater. Sci. Eng. C"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/22\/13999\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:17:18Z","timestamp":1760145438000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/22\/13999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,13]]},"references-count":58,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["ijms232213999"],"URL":"https:\/\/doi.org\/10.3390\/ijms232213999","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,13]]}}}