{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T13:25:47Z","timestamp":1770643547737,"version":"3.49.0"},"reference-count":35,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,26]],"date-time":"2022-11-26T00:00:00Z","timestamp":1669420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["SFRH\/BD\/137802\/2018"],"award-info":[{"award-number":["SFRH\/BD\/137802\/2018"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["SFRH\/BD\/47650\/2019"],"award-info":[{"award-number":["SFRH\/BD\/47650\/2019"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["NORTE-01-0145-FEDER-000051"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000051"]}]},{"name":"\u201cCancer Research on Therapy Resistance: From Basic Mechanisms to Novel Targets\u201d","award":["SFRH\/BD\/137802\/2018"],"award-info":[{"award-number":["SFRH\/BD\/137802\/2018"]}]},{"name":"\u201cCancer Research on Therapy Resistance: From Basic Mechanisms to Novel Targets\u201d","award":["SFRH\/BD\/47650\/2019"],"award-info":[{"award-number":["SFRH\/BD\/47650\/2019"]}]},{"name":"\u201cCancer Research on Therapy Resistance: From Basic Mechanisms to Novel Targets\u201d","award":["NORTE-01-0145-FEDER-000051"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000051"]}]},{"name":"\u201cRET-altered cancers: what are we missing?\u201d\u2013projeto de investiga\u00e7\u00e3o patrocinado pela Sociedade Portuguesa de Endocrinologia, diabetes e Metabolismo (SPEDM)","award":["SFRH\/BD\/137802\/2018"],"award-info":[{"award-number":["SFRH\/BD\/137802\/2018"]}]},{"name":"\u201cRET-altered cancers: what are we missing?\u201d\u2013projeto de investiga\u00e7\u00e3o patrocinado pela Sociedade Portuguesa de Endocrinologia, diabetes e Metabolismo (SPEDM)","award":["SFRH\/BD\/47650\/2019"],"award-info":[{"award-number":["SFRH\/BD\/47650\/2019"]}]},{"name":"\u201cRET-altered cancers: what are we missing?\u201d\u2013projeto de investiga\u00e7\u00e3o patrocinado pela Sociedade Portuguesa de Endocrinologia, diabetes e Metabolismo (SPEDM)","award":["NORTE-01-0145-FEDER-000051"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000051"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>DGCR8 emerged recently as miRNAs biogenesis pathway protein with a highlighted role in thyroid disease. This study aimed to characterize this miRNA biogenesis component, in particular the p.(E518K) mutation and DGCR8 expression in a series of thyroid lesions. The series of thyroid lesions was genotyped for the c.1552G&gt;A p.(E518K) mutation. When frozen tissue was available, DGCR8 mRNA expression was analysed by qPCR. Formalin-fixed paraffin-embedded tissues were studied for DGCR8 immunoexpression. We present for the first time the p.(E518K) mutation in a case of poorly differentiated thyroid carcinoma and present the deregulation of DGCR8 expression at mRNA level in follicular-patterned tumours. The obtained data solidify DGCR8 as another important player of miRNA-related gene mutations in thyroid tumorigenesis, particularly in follicular-patterned thyroid tumours.<\/jats:p>","DOI":"10.3390\/ijms232314812","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T04:58:50Z","timestamp":1669784330000},"page":"14812","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["DGCR8 Microprocessor Subunit Mutation and Expression Deregulation in Thyroid Lesions"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3718-1111","authenticated-orcid":false,"given":"Lia","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Escola Superior de Sa\u00fade do Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade da Universidade do Porto (I3S), Rua Alfredo Allen, 4200-135 Porto, Portugal"},{"name":"Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), Rua J\u00falio Amaral de Carvalho, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3736-1323","authenticated-orcid":false,"given":"Sule","family":"Canberk","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade da Universidade do Porto (I3S), Rua Alfredo Allen, 4200-135 Porto, Portugal"},{"name":"Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), Rua J\u00falio Amaral de Carvalho, 4200-135 Porto, Portugal"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas da Universidade do Porto (ICBAS), Rua Jorge Viterbo Ferreira, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7752-998X","authenticated-orcid":false,"given":"Sofia","family":"Macedo","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade da Universidade do Porto (I3S), Rua Alfredo Allen, 4200-135 Porto, Portugal"},{"name":"Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), Rua J\u00falio Amaral de Carvalho, 4200-135 Porto, Portugal"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas da Universidade do Porto (ICBAS), Rua Jorge Viterbo Ferreira, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9607-6998","authenticated-orcid":false,"given":"Paula","family":"Soares","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade da Universidade do Porto (I3S), Rua Alfredo Allen, 4200-135 Porto, Portugal"},{"name":"Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), Rua J\u00falio Amaral de Carvalho, 4200-135 Porto, Portugal"},{"name":"Faculdade de Medicina da Universidade do Porto, Alameda Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0871-1427","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Vinagre","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade da Universidade do Porto (I3S), Rua Alfredo Allen, 4200-135 Porto, Portugal"},{"name":"Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), Rua J\u00falio Amaral de Carvalho, 4200-135 Porto, Portugal"},{"name":"Faculdade de Medicina da Universidade do Porto, Alameda Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1007\/s00018-019-03020-9","article-title":"Dgcr8 knockout approaches to understand microRNA functions in vitro and in vivo","volume":"76","author":"Guo","year":"2019","journal-title":"Cell. 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