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Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7046","DOI":"10.1073\/pnas.0802463105","article-title":"Tissue-engineered endothelial and epithelial implants differentially and synergistically regulate airway repair","volume":"105","author":"Zani","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e000429","DOI":"10.1136\/bmjresp-2019-000429","article-title":"Prevalence and outcome of central airway obstruction in patients with lung cancer","volume":"6","author":"Daneshvar","year":"2019","journal-title":"BMJ Open Respir. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"794","DOI":"10.7150\/jca.15097","article-title":"Survival and Quality of Life Benefit after Endoscopic Management of Malignant Central Airway Obstruction","volume":"7","author":"Stratakos","year":"2016","journal-title":"J. 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Surg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1159\/000479164","article-title":"Towards Individualized Tracheobronchial Stents: Technical, Practical and Legal Considerations","volume":"94","author":"Freitag","year":"2017","journal-title":"Respiration"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.jobcr.2019.10.003","article-title":"Polycaprolactone as biomaterial for bone scaffolds: Review of literature","volume":"10","author":"Dwivedi","year":"2020","journal-title":"J. Oral Biol. Craniofacial Res."},{"key":"ref_17","first-page":"1","article-title":"Impact of sterilization by electron beam, gamma radiation and X-rays on electrospun poly-(\u03b5-caprolactone) fiber mats","volume":"30","author":"Hoheisel","year":"2019","journal-title":"J. Mater. Sci. Mater. 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PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0214956"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Castilla-Cort\u00e1zar, I., Vidaurre, A., Mar\u00ed, B., and Campillo-Fern\u00e1ndez, A.J. (2019). Morphology, Crystallinity, and Molecular Weight of Poly(\u03b5-caprolactone)\/Graphene Oxide Hybrids. Polymers, 11.","DOI":"10.3390\/polym11071099"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1002\/jbm.820190408","article-title":"The intracellular degradation of poly(\u03b5-caprolactone)","volume":"19","author":"Woodward","year":"1985","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8885","DOI":"10.1021\/acsomega.0c00503","article-title":"Impact of Various Sterilization and Disinfection Techniques on Electrospun Poly-\u03b5-caprolactone","volume":"5","author":"Horakova","year":"2020","journal-title":"ACS Omega"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Navarro, R., Burillo, G., Adem, E., and Marcos-Fern\u00e1ndez, A. (2018). Effect of Ionizing Radiation on the Chemical Structure and the Physical Properties of Polycaprolactones of Different Molecular Weight. Polymers, 10.","DOI":"10.3390\/polym10040397"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1080\/00914037.2014.977900","article-title":"Dose-Dependent Effects of Gamma Irradiation on the Materials Properties and Cell Proliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds","volume":"64","author":"Augustine","year":"2015","journal-title":"Int. J. Polym. Mater. Polym. Biomater."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1002\/polb.23072","article-title":"Gamma irradiation of electrospun poly(\u03b5-caprolactone) fibers affects material properties but not cell response","volume":"50","author":"Bosworth","year":"2012","journal-title":"J. Polym. Sci. Part B Polym. Phys."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.medengphy.2008.07.005","article-title":"Effect of sterilisation by gamma irradiation on the ability of polycaprolactone (PCL) to act as a scaffold material","volume":"31","author":"Cottam","year":"2009","journal-title":"Med. Eng. Phys."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sadasivuni, K.K., Ponnamma, D., Rajan, M., Ahmed, B., and Al-Maadeed, M.A.S. (2019). Polymer Nanocomposites in Biomedical Engineering, Springer.","DOI":"10.1007\/978-3-030-04741-2"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"283","DOI":"10.4028\/www.scientific.net\/AMM.890.283","article-title":"A Novel Biomanufacturing System to Produce Multi-Material Scaffolds for Tissue Engineering: Concept and Preliminary Results","volume":"890","author":"Viana","year":"2019","journal-title":"Appl. Mech. Mater."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Biscaia, S., Branquinho, M.V., Alvites, R.D., Fonseca, R., Sousa, A.C., Pedrosa, S.S., Caseiro, A.R., Guedes, F., Patr\u00edcio, T., and Viana, T. (2022). 3D Printed Poly (e-caprolactone)\/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on a Composite Preparation by Melt Blending or Solvent Casting Techniques and the Influence of Bioceramic Content on Scaffold Properties. Int. J. Mol. Sci., 23.","DOI":"10.20944\/preprints202201.0221.v1"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2021\/6613029","article-title":"Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem\/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo","volume":"2021","author":"Alvites","year":"2021","journal-title":"Stem Cells Int."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Branquinho, M., Ferreira, S., Alvites, R., Magueta, A., Ivanov, M., Sousa, A., Amorim, I., Faria, F., Fernandes, M., and Vilarinho, P. (2021). In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering\u2014A Preliminary Study. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22147655"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1007\/s10616-018-0225-5","article-title":"Dental pulp stem cells and osteogenesis: An update","volume":"70","author":"Mortada","year":"2018","journal-title":"Cytotechnology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1093\/rb\/rby025","article-title":"Dental pulp stem cells and Bonelike\u00ae for bone regeneration in ovine model","volume":"6","author":"Campos","year":"2018","journal-title":"Regen. Biomater."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Tapia-Guerrero, Y.S., Del Prado-Audelo, M.L., Borbolla-Jim\u00e9nez, F.V., Gomez, D.M.G., Garc\u00eda-Aguirre, I., Col\u00edn-Castro, C.A., Morales-Gonz\u00e1lez, J.A., Leyva-G\u00f3mez, G., and Maga\u00f1a, J.J. (2020). Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications. Materials, 13.","DOI":"10.3390\/ma13051090"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"133","DOI":"10.3390\/microarrays4020133","article-title":"3D Cell Culture in Alginate Hydrogels","volume":"4","author":"Andersen","year":"2015","journal-title":"Microarrays"},{"key":"ref_37","first-page":"910","article-title":"2D and 3D cell cultures\u2014A comparison of different types of cancer cell cultures","volume":"14","author":"Kolenda","year":"2018","journal-title":"Arch. Med. 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