{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T16:29:39Z","timestamp":1776702579030,"version":"3.51.2"},"reference-count":78,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"InfoSa\u00fade","award":["2020.08563.BD"],"award-info":[{"award-number":["2020.08563.BD"]}]},{"name":"Associa\u00e7\u00e3o Nacional das Farm\u00e1cias (ANF)","award":["2020.08563.BD"],"award-info":[{"award-number":["2020.08563.BD"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","doi-asserted-by":"publisher","award":["2020.08563.BD"],"award-info":[{"award-number":["2020.08563.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Physiologically, \u03b2-adrenoceptors are major regulators of lipid metabolism, which may be reflected in alterations in lipid droplet dynamics. \u03b2-adrenoceptors have also been shown to participate in breast cancer carcinogenesis. Since lipid droplets may be seen as a hallmark of cancer, the present study aimed to investigate the role of \u03b2-adrenoceptors in the regulation of lipid droplet dynamics in MCF-7 breast cancer cells. Cells were treated for up to 72 h with adrenaline (an endogenous adrenoceptor agonist), isoprenaline (a non-selective \u03b2-adrenoceptor agonist) and salbutamol (a selective \u03b22-selective agonist), and their effects on lipid droplets were evaluated using Nile Red staining. Adrenaline or isoprenaline, but not salbutamol, caused a lipid-accumulating phenotype in the MCF-7 cells. These effects were significantly reduced by selective \u03b21- and \u03b23-antagonists (10 nM atenolol and 100 nM L-748,337, respectively), indicating a dependence on both \u03b21- and \u03b23-adrenoceptors. These effects were dependent on the cAMP signalling pathway, involving both protein kinase A (PKA) and cAMP-dependent guanine-nucleotide-exchange (EPAC) proteins: treatment with cAMP-elevating agents (forskolin or 8-Br-cAMP) induced lipid droplet accumulation, whereas either 1 \u00b5M H-89 or 1 \u00b5M ESI-09 (PKA or EPAC inhibitors, respectively) abrogated this effect. Taken together, the present results demonstrate the existence of a \u03b2-adrenoceptor-mediated regulation of lipid droplet dynamics in breast cancer cells, likely involving \u03b21- and \u03b23-adrenoceptors, revealing a new mechanism by which adrenergic stimulation may influence cancer cell metabolism.<\/jats:p>","DOI":"10.3390\/ijms24010767","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T04:17:38Z","timestamp":1672633058000},"page":"767","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Activation of \u03b2-Adrenoceptors Promotes Lipid Droplet Accumulation in MCF-7 Breast Cancer Cells via cAMP\/PKA\/EPAC Pathways"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6800-9621","authenticated-orcid":false,"given":"Dany","family":"Silva","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"UCIBIO-REQUIMTE, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"given":"Katarzyna","family":"Kacprzak","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"given":"Clara","family":"Quintas","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"UCIBIO-REQUIMTE, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3045-2150","authenticated-orcid":false,"given":"Jorge","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"UCIBIO-REQUIMTE, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6705-7798","authenticated-orcid":false,"given":"Paula","family":"Fresco","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"UCIBIO-REQUIMTE, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/onc.2015.58","article-title":"beta2-AR signaling controls trastuzumab resistance-dependent pathway","volume":"35","author":"Liu","year":"2016","journal-title":"Oncogene"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1002\/jcp.30707","article-title":"Contribution of adrenergic mechanisms for the stress-induced breast cancer carcinogenesis","volume":"237","author":"Silva","year":"2022","journal-title":"J. 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