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Although elevated air [CO2] might mitigate some of these effects in plants, the mechanisms underlying the involved responses are poorly understood in woody economically important crops such as Coffea. This study analyzed transcriptome changes in Coffea canephora cv. CL153 and C. arabica cv. Icatu exposed to moderate (MWD) or severe water deficits (SWD) and grown under ambient (aCO2) or elevated (eCO2) air [CO2]. We found that changes in expression levels and regulatory pathways were barely affected by MWD, while the SWD condition led to a down-regulation of most differentially expressed genes (DEGs). eCO2 attenuated the impacts of drought in the transcripts of both genotypes but mostly in Icatu, in agreement with physiological and metabolic studies. A predominance of protective and reactive oxygen species (ROS)-scavenging-related genes, directly or indirectly associated with ABA signaling pathways, was found in Coffea responses, including genes involved in water deprivation and desiccation, such as protein phosphatases in Icatu, and aspartic proteases and dehydrins in CL153, whose expression was validated by qRT-PCR. The existence of a complex post-transcriptional regulatory mechanism appears to occur in Coffea explaining some apparent discrepancies between transcriptomic, proteomic, and physiological data in these genotypes.<\/jats:p>","DOI":"10.3390\/ijms24043210","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T01:56:48Z","timestamp":1675735008000},"page":"3210","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Overexpression of Water-Responsive Genes Promoted by Elevated CO2 Reduces ROS and Enhances Drought Tolerance in Coffea Species"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9788-4831","authenticated-orcid":false,"given":"Isabel","family":"Marques","sequence":"first","affiliation":[{"name":"Plant-Environment Interactions and Biodiversity Lab (PlantStress & Biodiversity), Forest Research Centre (CEF), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-017 Lisboa, Portugal"},{"name":"Associate Laboratory TERRA, Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1485-5942","authenticated-orcid":false,"given":"Isabel","family":"Fernandes","sequence":"additional","affiliation":[{"name":"cE3c\u2014Center for Ecology, Evolution and Environmental Changes and CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5408-5212","authenticated-orcid":false,"given":"Oct\u00e1vio S.","family":"Paulo","sequence":"additional","affiliation":[{"name":"cE3c\u2014Center for Ecology, Evolution and Environmental Changes and CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}]},{"given":"Dora","family":"Batista","sequence":"additional","affiliation":[{"name":"Associate Laboratory TERRA, Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-017 Lisboa, Portugal"},{"name":"Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-017 Lisboa, Portugal"}]},{"given":"Fernando C.","family":"Lidon","sequence":"additional","affiliation":[{"name":"Unidade de Geobioci\u00eancias, Geoengenharias e Geotecnologias (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8830-0846","authenticated-orcid":false,"given":"F\u00e1bio","family":"Partelli","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio do Norte do Esp\u00edrito Santo (CEUNES), Departmento Ci\u00eancias Agr\u00e1rias e Biol\u00f3gicas (DCAB), Universidade Federal Esp\u00edrito Santo (UFES), S\u00e3o Mateus 29932-540, ES, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9637-8475","authenticated-orcid":false,"given":"F\u00e1bio M.","family":"DaMatta","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Vegetal, Universidade Federal Vi\u00e7osa (UFV), Vi\u00e7osa 36570-900, MG, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6071-6460","authenticated-orcid":false,"given":"Ana I.","family":"Ribeiro-Barros","sequence":"additional","affiliation":[{"name":"Plant-Environment Interactions and Biodiversity Lab (PlantStress & Biodiversity), Forest Research Centre (CEF), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-017 Lisboa, Portugal"},{"name":"Associate Laboratory TERRA, Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-017 Lisboa, Portugal"},{"name":"Unidade de Geobioci\u00eancias, Geoengenharias e Geotecnologias (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7639-7214","authenticated-orcid":false,"given":"Jos\u00e9 C.","family":"Ramalho","sequence":"additional","affiliation":[{"name":"Plant-Environment Interactions and Biodiversity Lab (PlantStress & Biodiversity), Forest Research Centre (CEF), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-017 Lisboa, Portugal"},{"name":"Associate Laboratory TERRA, Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-017 Lisboa, Portugal"},{"name":"Unidade de Geobioci\u00eancias, Geoengenharias e Geotecnologias (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1073\/pnas.0812721106","article-title":"Irreversible climate change due to carbon dioxide emissions","volume":"106","author":"Solomon","year":"2009","journal-title":"Proc. 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