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2020","award":["UIDP\/04326\/2020"],"award-info":[{"award-number":["UIDP\/04326\/2020"]}]},{"name":"CRESC Algarve 2020 and COMPETE 2020","award":["LA\/P\/0101\/2020"],"award-info":[{"award-number":["LA\/P\/0101\/2020"]}]},{"name":"CRESC Algarve 2020 and COMPETE 2020","award":["EMBRC.PT ALG-01-0145-FEDER-02212"],"award-info":[{"award-number":["EMBRC.PT ALG-01-0145-FEDER-02212"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ectopic calcification refers to the pathological accumulation of calcium ions in soft tissues and is often the result of a dysregulated action or disrupted function of proteins involved in extracellular matrix mineralization. While the mouse has traditionally been the go-to model organism for the study of pathologies associated with abnormal calcium deposition, many mouse mutants often have exacerbated phenotypes and die prematurely, limiting the understanding of the disease and the development of effective therapies. Since the mechanisms underlying ectopic calcification share some analogy with those of bone formation, the zebrafish (Danio rerio)\u2014a well-established model for studying osteogenesis and mineralogenesis\u2014has recently gained momentum as a model to study ectopic calcification disorders. In this review, we outline the mechanisms of ectopic mineralization in zebrafish, provide insights into zebrafish mutants that share phenotypic similarities with human pathological mineralization disorders, list the compounds capable of rescuing mutant phenotypes, and describe current methods to induce and characterize ectopic calcification in zebrafish.<\/jats:p>","DOI":"10.3390\/ijms24043366","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T05:37:31Z","timestamp":1675834651000},"page":"3366","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Zebrafish Models to Study Ectopic Calcification and Calcium-Associated Pathologies"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1218-368X","authenticated-orcid":false,"given":"Jo\u00e3o M. A.","family":"Santos","sequence":"first","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9565-9198","authenticated-orcid":false,"given":"Vincent","family":"Laiz\u00e9","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9582-1957","authenticated-orcid":false,"given":"Paulo J.","family":"Gavaia","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal"}]},{"given":"Nat\u00e9rcia","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Algarve Biomedical Center, University of Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3114-6662","authenticated-orcid":false,"given":"M. Leonor","family":"Cancela","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Algarve Biomedical Center, University of Algarve, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7762\/cnr.2015.4.1.1","article-title":"The role of calcium in human aging","volume":"4","author":"Beto","year":"2015","journal-title":"Clin. Nutr. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1152\/physrev.2000.80.3.1215","article-title":"Calcium ion in skeletal muscle: Its crucial role for muscle function, plasticity, and disease","volume":"80","author":"Berchtold","year":"2000","journal-title":"Physiol. 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