{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T10:11:57Z","timestamp":1760609517992,"version":"build-2065373602"},"reference-count":66,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,18]],"date-time":"2023-02-18T00:00:00Z","timestamp":1676678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04293\/2020","SFRH\/BD\/135921\/2018","IF\/00889\/2015","ROTEIRO\/0028\/2013","LISBOA-01-0145-FEDER-022125"],"award-info":[{"award-number":["UIDB\/04293\/2020","SFRH\/BD\/135921\/2018","IF\/00889\/2015","ROTEIRO\/0028\/2013","LISBOA-01-0145-FEDER-022125"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Fundo Social Europeu and Programa Operacional Potencial Humano","doi-asserted-by":"publisher","award":["UIDB\/04293\/2020","SFRH\/BD\/135921\/2018","IF\/00889\/2015","ROTEIRO\/0028\/2013","LISBOA-01-0145-FEDER-022125"],"award-info":[{"award-number":["UIDB\/04293\/2020","SFRH\/BD\/135921\/2018","IF\/00889\/2015","ROTEIRO\/0028\/2013","LISBOA-01-0145-FEDER-022125"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Portuguese Mass Spectrometry Network","award":["UIDB\/04293\/2020","SFRH\/BD\/135921\/2018","IF\/00889\/2015","ROTEIRO\/0028\/2013","LISBOA-01-0145-FEDER-022125"],"award-info":[{"award-number":["UIDB\/04293\/2020","SFRH\/BD\/135921\/2018","IF\/00889\/2015","ROTEIRO\/0028\/2013","LISBOA-01-0145-FEDER-022125"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cdh1p is one of the two substrate adaptor proteins of the anaphase promoting complex\/cyclosome (APC\/C), a ubiquitin ligase that regulates proteolysis during cell cycle. In this work, using a proteomic approach, we found 135 mitochondrial proteins whose abundance was significantly altered in the cdh1\u0394 mutant, with 43 up-regulated proteins and 92 down-regulated proteins. The group of significantly up-regulated proteins included subunits of the mitochondrial respiratory chain, enzymes from the tricarboxylic acid cycle and regulators of mitochondrial organization, suggesting a metabolic remodelling towards an increase in mitochondrial respiration. In accordance, mitochondrial oxygen consumption and Cytochrome c oxidase activity increased in Cdh1p-deficient cells. These effects seem to be mediated by the transcriptional activator Yap1p, a major regulator of the yeast oxidative stress response. YAP1 deletion suppressed the increased Cyc1p levels and mitochondrial respiration in cdh1\u0394 cells. In agreement, Yap1p is transcriptionally more active in cdh1\u0394 cells and responsible for the higher oxidative stress tolerance of cdh1\u0394 mutant cells. Overall, our results unveil a new role for APC\/C-Cdh1p in the regulation of the mitochondrial metabolic remodelling through Yap1p activity.<\/jats:p>","DOI":"10.3390\/ijms24044111","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T04:28:57Z","timestamp":1676867337000},"page":"4111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The APC\/C Activator Cdh1p Plays a Role in Mitochondrial Metabolic Remodelling in Yeast"],"prefix":"10.3390","volume":"24","author":[{"given":"Ana Cl\u00e1udia","family":"Leite","sequence":"first","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"IBMC\u2014Instituto de Biologia Celular e Molecular, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"ICBAS\u2014Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"given":"Maria","family":"Barbedo","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"IBMC\u2014Instituto de Biologia Celular e Molecular, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7868-4663","authenticated-orcid":false,"given":"V\u00edtor","family":"Costa","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"IBMC\u2014Instituto de Biologia Celular e Molecular, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"ICBAS\u2014Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8167-6912","authenticated-orcid":false,"given":"Clara","family":"Pereira","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"},{"name":"IBMC\u2014Instituto de Biologia Celular e Molecular, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,18]]},"reference":[{"key":"ref_1","first-page":"1160","article-title":"Glucose repression of yeast mitochondrial transcription: Kinetics of derepression and role of nuclear genes","volume":"14","author":"Ulery","year":"1994","journal-title":"Mol. 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