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spinal cord injury (SCI) initiates a cascade of cellular events, culminating in irreversible tissue loss and neuroinflammation. After the trauma, the blood vessels are destroyed. The blood-spinal cord barrier (BSCB), a physical barrier between the blood and spinal cord parenchyma, is disrupted, facilitating the infiltration of immune cells, and contributing to a toxic spinal microenvironment, affecting axonal regeneration. Understanding how the vascular constituents of the BSCB respond to injury is crucial to prevent BSCB impairment and to improve spinal cord repair. Here, we focus our attention on the vascular transcriptome at 3- and 7-days post-injury (dpi), during which BSCB is abnormally leaky, to identify potential molecular players that are injury-specific. Using the mouse contusion model, we identified Cd9 and Mylip genes as differentially expressed at 3 and 7 dpi. CD9 and MYLIP expression were injury-induced on vascular cells, endothelial cells and pericytes, at the injury epicentre at 7 dpi, with a spatial expression predominantly at the caudal region of the lesion. These results establish CD9 and MYLIP as two new potential players after SCI, and future studies targeting their expression might bring promising results for spinal cord repair.<\/jats:p>","DOI":"10.3390\/ijms24076433","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T02:23:46Z","timestamp":1680143026000},"page":"6433","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Mouse Spinal Cord Vascular Transcriptome Analysis Identifies CD9 and MYLIP as Injury-Induced Players"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1329-7769","authenticated-orcid":false,"given":"Isaura","family":"Martins","sequence":"first","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2342-5354","authenticated-orcid":false,"given":"Dalila","family":"Neves-Silva","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Mariana","family":"Ascens\u00e3o-Ferreira","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Ana Filipa","family":"Dias","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3660-9646","authenticated-orcid":false,"given":"Daniel","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Ana Filipa","family":"Isidro","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Raquel","family":"Quit\u00e9ria","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Diogo","family":"Paramos-de-Carvalho","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Nuno L.","family":"Barbosa-Morais","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Leonor","family":"Sa\u00fade","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes e Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s13311-018-0631-6","article-title":"Spinal Cord Injury Scarring and Inflammation: Therapies Targeting Glial and Inflammatory Responses","volume":"15","author":"Orr","year":"2018","journal-title":"Neurotherapeutics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3879","DOI":"10.1038\/s41467-019-11707-7","article-title":"Moving beyond the Glial Scar for Spinal Cord Repair","volume":"10","author":"Bradbury","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1172\/JCI90608","article-title":"V Cell Biology of Spinal Cord Injury and Repair","volume":"127","author":"Burda","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.neuron.2013.12.034","article-title":"V Reactive Gliosis and the Multicellular Response to CNS Damage and Disease","volume":"81","author":"Burda","year":"2014","journal-title":"Neuron"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.wneu.2021.07.148","article-title":"Inflammatory Response to Spinal Cord Injury and Its Treatment","volume":"155","author":"Liu","year":"2021","journal-title":"World Neurosurg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1152\/physrev.00017.2017","article-title":"The Biology of Regeneration Failure and Success after Spinal Cord Injury","volume":"98","author":"Tran","year":"2018","journal-title":"Physiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"113704","DOI":"10.1016\/j.expneurol.2021.113704","article-title":"Neuroimmunological Therapies for Treating Spinal Cord Injury: Evidence and Future Perspectives","volume":"341","author":"Chio","year":"2021","journal-title":"Exp. Neurol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1111\/apha.12250","article-title":"The Neurovascular Unit\u2014Concept Review","volume":"210","author":"Muoio","year":"2014","journal-title":"Acta Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1097\/WCO.0000000000000550","article-title":"Emerging Targets for Reprograming the Immune Response to Promote Repair and Recovery of Function after Spinal Cord Injury","volume":"31","author":"Brennan","year":"2018","journal-title":"Curr. Opin. Neurol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"44105","DOI":"10.1088\/1748-605X\/aaa9d8","article-title":"Biomaterials for Revascularization and Immunomodulation after Spinal Cord Injury","volume":"13","author":"Haggerty","year":"2018","journal-title":"Biomed. Mater."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2438","DOI":"10.4049\/jimmunol.1400401","article-title":"Neutrophils Mediate Blood\u2013Spinal Cord Barrier Disruption in Demyelinating Neuroinflammatory Diseases","volume":"193","author":"Chamma","year":"2014","journal-title":"J. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1126\/science.1203165","article-title":"A Pericyte Origin of Spinal Cord Scar Tissue","volume":"333","author":"Dias","year":"2011","journal-title":"Science"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/s13311-011-0029-1","article-title":"Targeting Microvasculature for Neuroprotection after SCI","volume":"8","author":"Fassbender","year":"2011","journal-title":"Neurotherapeutics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1002\/jnr.10759","article-title":"Blood-Spinal Cord Barrier after Spinal Cord Injury: Relation to Revascularization and Wound Healing","volume":"74","author":"Whetstone","year":"2003","journal-title":"J. Neurosci. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1089\/neu.2006.0149","article-title":"Immediate Damage to the Blood-Spinal Cord Barrier Due to Mechanical Trauma","volume":"24","author":"Maikos","year":"2007","journal-title":"J. Neurotrauma"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1089\/neu.2020.7413","article-title":"Blood-Spinal Cord Barrier in Spinal Cord Injury: A Review","volume":"38","author":"Jin","year":"2021","journal-title":"J. Neurotrauma"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"631500","DOI":"10.3389\/fphys.2021.631500","article-title":"Revascularization after Traumatic Spinal Cord Injury","volume":"12","author":"Yao","year":"2021","journal-title":"Front. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/s41587-019-0114-2","article-title":"Determining Cell Type Abundance and Expression from Bulk Tissues with Digital Cytometry","volume":"37","author":"Newman","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.cell.2018.06.021","article-title":"Molecular Architecture of the Mouse Nervous System","volume":"174","author":"Zeisel","year":"2018","journal-title":"Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene Set Enrichment Analysis: A Knowledge-Based Approach for Interpreting Genome-Wide Expression Profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"282","DOI":"10.3389\/fneur.2019.00282","article-title":"Traumatic Spinal Cord Injury: An Overview of Pathophysiology, Models and Acute Injury Mechanisms","volume":"10","author":"Alizadeh","year":"2019","journal-title":"Front. Neurol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1002\/cyto.a.22677","article-title":"Isolation of Vascular Endothelial Cells from Intact and Injured Murine Brain Cortex\u2014Technical Issues and Pitfalls in FACS Analysis of the Nervous Tissue","volume":"87","author":"Wylot","year":"2015","journal-title":"Cytom. Part A"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1161\/ATVBAHA.107.151456","article-title":"PECAM-1: A Multi-Functional Molecule in Inflammation and Vascular Biology","volume":"27","author":"Woodfin","year":"2007","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1074\/jbc.M207805200","article-title":"A Functionally Relevant Conformational Epitope on the CD9 Tetraspanin Depends on the Association with Activated Beta1 Integrin","volume":"278","author":"Ovalle","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"863","DOI":"10.3389\/fimmu.2018.00863","article-title":"Tetraspanin CD9: A Key Regulator of Cell Adhesion in the Immune System","volume":"9","author":"Reyes","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2852","DOI":"10.1182\/blood-2004-09-3606","article-title":"Endothelial Tetraspanin Microdomains Regulate Leukocyte Firm Adhesion during Extravasation","volume":"105","author":"Barreiro","year":"2005","journal-title":"Blood"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1083\/jcb.200805076","article-title":"Endothelial Adhesion Receptors Are Recruited to Adherent Leukocytes by Inclusion in Preformed Tetraspanin Nanoplatforms","volume":"183","author":"Barreiro","year":"2008","journal-title":"J. Cell Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1111\/jnc.13397","article-title":"Nerve Growth Factor (NGF) and pro-NGF Increase Low-Density Lipoprotein (LDL) Receptors in Neuronal Cells Partly by Different Mechanisms: Role of LDL in Neurite Outgrowth","volume":"136","author":"Do","year":"2016","journal-title":"J. Neurochem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/1878-0261.13016","article-title":"Upregulated Functional Gene Expression Programmes in Tumour Pericytes Mark Progression in Patients with Low-Grade Glioma","volume":"16","author":"Oudenaarden","year":"2022","journal-title":"Mol. Oncol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1161\/01.RES.0000182903.16652.d7","article-title":"Endothelial\/Pericyte Interactions","volume":"97","author":"Armulik","year":"2005","journal-title":"Circ. Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Cucu, I., and Nicolescu, M.I. (2021). A Synopsis of Signaling Crosstalk of Pericytes and Endothelial Cells in Salivary Gland. Dent. J., 9.","DOI":"10.3390\/dj9120144"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Sharma, K., Zhang, Y., Paudel, K.R., Kachelmeier, A., Hansbro, P.M., and Shi, X. (2022). The Emerging Role of Pericyte-Derived Extracellular Vesicles in Vascular and Neurological Health. Cells, 11.","DOI":"10.3390\/cells11193108"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"21607","DOI":"10.1038\/srep21607","article-title":"High-Throughput Proteomics Reveal Alarmins as Amplifiers of Tissue Pathology and Inflammation after Spinal Cord Injury","volume":"6","author":"Didangelos","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"117235","DOI":"10.1016\/j.lfs.2019.117235","article-title":"Searching for Differentially Expressed Proteins in Spinal Cord Injury Based on the Proteomics Analysis","volume":"242","author":"Ding","year":"2020","journal-title":"Life Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Bhattacharya, S., Myers, J., Baker, C., Guo, M., Danopoulos, S., Myers, J., Bandyopadhyay, G., Romas, S., Huyck, H., and Misra, R. (2020). Single Cell Transcriptomic Profiling Identifies Molecular Phenotypes of Newborn Human Lung Cells. bioRxiv.","DOI":"10.1101\/2020.06.16.156042"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1038\/nm.4331","article-title":"Pericytes Impair Capillary Blood Flow and Motor Function after Chronic Spinal Cord Injury","volume":"23","author":"Li","year":"2017","journal-title":"Nat. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"64330","DOI":"10.18632\/oncotarget.19278","article-title":"MiR-19b Promotes Breast Cancer Metastasis through Targeting MYLIP and Its Related Cell Adhesion Molecules","volume":"8","author":"Zhao","year":"2017","journal-title":"Oncotarget"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/nprot.2014.006","article-title":"Full-Length RNA-Seq from Single Cells Using Smart-Seq2","volume":"9","author":"Picelli","year":"2014","journal-title":"Nat. Protoc."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","article-title":"MultiQC: Summarize Analysis Results for Multiple Tools and Samples in a Single Report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1016\/j.cell.2014.11.035","article-title":"A Highly Conserved Program of Neuronal Microexons Is Misregulated in Autistic Brains","volume":"159","author":"Irimia","year":"2014","journal-title":"Cell"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1101\/gr.220962.117","article-title":"An Atlas of Alternative Splicing Profiles and Functional Associations Reveals New Regulatory Programs and Genes That Simultaneously Express Multiple Major Isoforms","volume":"27","author":"Tapial","year":"2017","journal-title":"Genome Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"EdgeR: A Bioconductor Package for Differential Expression Analysis of Digital Gene Expression Data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"Limma Powers Differential Expression Analyses for RNA-Sequencing and Microarray Studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"ClusterProfiler: An R Package for Comparing Biological Themes among Gene Clusters","volume":"16","author":"Yu","year":"2012","journal-title":"OMICS J. Integr. Biol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/7\/6433\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:06:16Z","timestamp":1760123176000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/7\/6433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,29]]},"references-count":44,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["ijms24076433"],"URL":"https:\/\/doi.org\/10.3390\/ijms24076433","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,29]]}}}