{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T19:23:40Z","timestamp":1774466620544,"version":"3.50.1"},"reference-count":102,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T00:00:00Z","timestamp":1689033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The exposure of skin cells to UV radiation leads to redox imbalances and inflammation. The present study investigates a lipid extract obtained from the microalga Nannochloropsis oceanica as a potential protector against UVB-induced disturbances in human keratinocytes. The findings of this study show that the Nannochloropsis oceanica extract significantly inhibits UVB-induced cell death while concurrently decreasing the activity of pro-oxidative enzymes (xanthine and NADPH oxidase) and reducing the levels of ROS. Furthermore, the extract augments the activity of antioxidant enzymes (superoxide dismutases and catalase), as well as glutathione\/thioredoxin-dependent systems in UVB-irradiated cells. The expression of Nrf2 factor activators (p62, KAP1, p38) was significantly elevated, while no impact was observed on Nrf2 inhibitors (Keap1, Bach1). The antioxidant activity of the extract was accompanied by the silencing of overexpressed membrane transporters caused by UVB radiation. Furthermore, the Nannochloropsis oceanica extract exhibited anti-inflammatory effects in UVB-irradiated keratinocytes by decreasing the levels of TNF\u03b1, 8-iso prostaglandin F2, and 4-HNE-protein adducts. In conclusion, the lipid components of Nannochloropsis oceanica extract effectively prevent the pro-oxidative and pro-inflammatory effects of UVB radiation in keratinocytes, thereby stabilizing the natural metabolism of skin cells.<\/jats:p>","DOI":"10.3390\/ijms241411302","type":"journal-article","created":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T00:50:41Z","timestamp":1689123041000},"page":"11302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Prevention of UVB Induced Metabolic Changes in Epidermal Cells by Lipid Extract from Microalgae Nannochloropsis oceanica"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5766-6271","authenticated-orcid":false,"given":"Anna","family":"Stasiewicz","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-069 Bialystok, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1125-3885","authenticated-orcid":false,"given":"Tiago","family":"Conde","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre, LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Santiago University Campus, 3810-193 Aveiro, Portugal"},{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, Santiago University Campus, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5240-1346","authenticated-orcid":false,"given":"Agnieszka","family":"G\u0119gotek","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-069 Bialystok, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5357-3601","authenticated-orcid":false,"given":"Maria Ros\u00e1rio","family":"Domingues","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre, LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Santiago University Campus, 3810-193 Aveiro, Portugal"},{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, Santiago University Campus, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8060-7675","authenticated-orcid":false,"given":"Pedro","family":"Domingues","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre, LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Santiago University Campus, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5397-7139","authenticated-orcid":false,"given":"El\u017cbieta","family":"Skrzydlewska","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-069 Bialystok, Poland"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/srt.12424","article-title":"The relationship between skin function barrier properties and body-dependent factors","volume":"24","author":"Spano","year":"2018","journal-title":"Skin Res. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"28730","DOI":"10.1007\/s11356-020-09280-4","article-title":"The impact of airborne pollution and exposure to solar ultraviolet radiation on skin: Mechanistic and physiological insight","volume":"27","author":"Ali","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0076-6879(00)19035-4","article-title":"Photocarcinogenesis: UVA vs. UVB","volume":"319","year":"2000","journal-title":"Methods Enzymol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"101036","DOI":"10.1016\/j.arr.2020.101036","article-title":"Biomarkers oxidative stress and autophagy in skin aging","volume":"59","author":"Gu","year":"2020","journal-title":"Ageing Res. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.taap.2018.09.038","article-title":"Roles of the KEAP1-NRF2 system in mammalian skin exposed to UV radiation","volume":"360","author":"Ikehata","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17023","DOI":"10.1038\/sigtrans.2017.23","article-title":"NF-\u03baB signaling in inflammation","volume":"2","author":"Liu","year":"2017","journal-title":"Signal Transduct. Target Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.5507\/bp.2006.003","article-title":"Ultraviolet light induced alteration to the skin","volume":"150","author":"Svobodova","year":"2006","journal-title":"Biomed. Pap. Med. Fac. Univ. Palacky. Olomouc. Czech Repub."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1016\/j.jaad.2012.09.053","article-title":"Pathobiology of actinic keratosis: Ultraviolet-dependent keratinocyte proliferation","volume":"68","author":"Berman","year":"2013","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1111\/exd.14260","article-title":"Skin pigmentation and its control: From ultraviolet radiation to stem cells","volume":"30","author":"Fisher","year":"2021","journal-title":"Exp. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Nakai, K., and Tsuruta, D. (2021). What are reactive oxygen species free radicals and oxidative stress in skin diseases?. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910799"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1038\/s41573-021-00233-1","article-title":"Targeting oxidative stress in disease: Promise and limitations of antioxidant therapy","volume":"20","author":"Forman","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_12","first-page":"2082145","article-title":"The Role of Oxidative Stress in Physiopathology and Pharmacological Treatment with Pro- And Antioxidant Properties in Chronic Diseases","volume":"2020","year":"2020","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Tungmunnithum, D., Thongboonyou, A., Pholboon, A., and Yangsabai, A. (2018). Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines, 5.","DOI":"10.3390\/medicines5030093"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Pereira, L. (2018). Seaweeds as source of bioactive substances and skin care therapy-Cosmeceuticals algotheraphy and thalassotherapy. Cosmetics, 5.","DOI":"10.3390\/cosmetics5040068"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1080\/10715762.2017.1355550","article-title":"Marine algae as attractive source to skin care","volume":"51","author":"Berthon","year":"2017","journal-title":"Free Radic Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.3389\/fphar.2020.01086","article-title":"Microalgae as Potential Anti-Inflammatory Natural Product Against Human Inflammatory Skin Diseases","volume":"11","author":"Choo","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1016\/j.biortech.2017.05.198","article-title":"Potential biomedical applications of marine algae","volume":"244","author":"Wang","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"20200189","DOI":"10.1002\/VIW.20200189","article-title":"Algae: A natural active material for biomedical applications","volume":"2","author":"Zhong","year":"2021","journal-title":"VIEW"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Mourelle, M., G\u00f3mez, C., and Legido, J. (2017). The Potential Use of Marine Microalgae and Cyanobacteria in Cosmetics and Thalassotherapy. Cosmetics, 4.","DOI":"10.3390\/cosmetics4040046"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/1475-2859-11-96","article-title":"Microalgal biofactories: A promising approach towards sustainable omega-3 fatty acid production","volume":"11","author":"Lim","year":"2012","journal-title":"Microb. Cell Fact."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"648","DOI":"10.3389\/fimmu.2020.00648","article-title":"Interplay between keratinocytes and fibroblasts: A systematic review providing a new angle for understanding skin fibrotic disorders","volume":"11","author":"Russo","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Conde, T.A., Zabetakis, I., Tsoupras, A., Medina, I., Costa, M., Silva, J., Neves, B., Domingues, P., and Domingues, M.R. (2021). Microalgal lipid extracts have potential to modulate the inflammatory response: A critical review. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22189825"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.phytochem.2014.02.004","article-title":"Polar lipids from the marine macroalga Palmaria palmata inhibit lipopolysaccharide-induced nitric oxide production in RAW264,7 macrophage cells","volume":"101","author":"Banskota","year":"2014","journal-title":"Phytochemistry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5402","DOI":"10.3390\/md13085402","article-title":"The anti-inflammatory effect of algae-derived lipid extracts on lipopolysaccharide (LPS)-stimulated human THP-1 macrophages","volume":"13","author":"Robertson","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"273","DOI":"10.2216\/i0031-8884-41-3-273.1","article-title":"Taxonomic characterization of a marine Nannochloropsis species, N. oceanica sp. nov. (Eustigmatophyceae)","volume":"41","author":"Suda","year":"2002","journal-title":"Phycologia"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1002\/bit.22033","article-title":"Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor","volume":"102","author":"Rodolfi","year":"2009","journal-title":"Biotechnol. Bioeng."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Cabrita, A.R., Guilherme-Fernandes, J., Valente, I.M., Almeida, A., Lima, S.A., Fonseca, A.J., and Maia, M.R. (2022). Nutritional Composition and Untargeted Metabolomics Reveal the Potential of Tetradesmus obliquus, Chlorella vulgaris and Nannochloropsis oceanica as Valuable Nutrient Sources for Dogs. Animals, 12.","DOI":"10.3390\/ani12192643"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"126627","DOI":"10.1016\/j.foodchem.2020.126627","article-title":"Microalgae Nannochloropsis oceanica as a future new natural source of vitamin D3","volume":"320","author":"Ljubic","year":"2020","journal-title":"Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"109053","DOI":"10.1016\/j.meatsci.2022.109053","article-title":"Nannochloropsis oceanica microalga feeding increases long-chain omega-3 polyunsaturated fatty acids in lamb meat","volume":"197","author":"Godinho","year":"2023","journal-title":"Meat Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Du Preez, R., Majzoub, M.E., Thomas, T., Panchal, S.K., and Brown, L. (2021). Nannochloropsis oceanica as a microalgal food intervention in diet-induced metabolic syndrome in rats. Nutrients, 13.","DOI":"10.3390\/nu13113991"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Conde, T., Lopes, D., \u0141uczaj, W., Neves, B., Pinto, B., Maur\u00edcio, T., Domingues, P., Skrzydlewska, E., and Domingues, M.R. (2022). Algal lipids as modulators of skin disease: A critical review. Metabolites, 12.","DOI":"10.3390\/metabo12020096"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1111\/php.13030","article-title":"The protective effect of violaxanthin from Nannochloropsis oceanica against ultraviolet B-induced damage in normal human dermal fibroblasts","volume":"95","author":"Kim","year":"2019","journal-title":"Photochem. Photobiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Ruiz, M., Mart\u00ednez-Gonz\u00e1lez, C.A., Kim, D.H., Santiesteban-Romero, B., Reyes-Pardo, H., Villase\u00f1or-Zepeda, K.R., Mel\u00e9ndez-S\u00e1nchez, E.R., Ram\u00edrez-Gamboa, D., D\u00edaz-Zamorano, A.L., and Sosa-Hern\u00e1ndez, J.E. (2022). Microalgae Bioactive Compounds to Topical Applications Products\u2014A Review. Molecules, 27.","DOI":"10.3390\/molecules27113512"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jdermsci.2015.11.005","article-title":"The cross-talk between electrophiles antioxidant defence and the endocannabinoid system in fibroblasts and keratinocytes after UVA and UVB irradiation","volume":"81","author":"Gegotek","year":"2016","journal-title":"J. Dermatol. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"113656","DOI":"10.1016\/j.jpba.2020.113656","article-title":"Therapeutic application of cannabidiol on UVA and UVB irradiated rat skin. A proteomic study","volume":"192","author":"Atalay","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s00403-019-01898-w","article-title":"Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation","volume":"311","author":"Skrzydlewska","year":"2019","journal-title":"Arch. Dermatol. Res."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Jastrz\u0105b, A., G\u0119gotek, A., and Skrzydlewska, E. (2019). Cannabidiol Regulates the Expression of Keratinocyte Proteins Involved in the Inflammation Process through Transcriptional Regulation. Cells, 8.","DOI":"10.3390\/cells8080827"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1083\/jcb.201708007","article-title":"Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling","volume":"217","author":"Wang","year":"2018","journal-title":"J. Cell Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.freeradbiomed.2016.11.033","article-title":"Antioxidants and HNE in redox homeostasis","volume":"111","author":"Skrzydlewska","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.prostaglandins.2013.04.003","article-title":"Isoprostanes and neuroprostanes: Total synthesis biological activity and biomarkers of oxidative stress in humans","volume":"107","author":"Galano","year":"2013","journal-title":"Prostaglandins Lipid Mediat."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.jdermsci.2018.02.002","article-title":"Proteins involved in the antioxidant and inflammatory response in rutin-treated human skin fibroblasts exposed to UVA or UVB irradiation","volume":"90","author":"Domingues","year":"2018","journal-title":"J. Dermatol. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1007\/s00011-022-01598-8","article-title":"Photoaging: UV radiation-induced inflammation and immunosuppression accelerate the aging process in the skin","volume":"71","author":"Salminen","year":"2022","journal-title":"Inflamm. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"102718","DOI":"10.1016\/j.algal.2022.102718","article-title":"Effects of outdoor and indoor cultivation on the polar lipid composition and antioxidant activity of Nannochloropsis oceanica and Nannochloropsis limnetica: A lipidomics perspective","volume":"64","author":"Couto","year":"2022","journal-title":"Algal Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"107","DOI":"10.2165\/00128071-200304020-00004","article-title":"Ceramides and skin function","volume":"4","author":"Coderch","year":"2003","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5456","DOI":"10.1016\/j.febslet.2006.08.039","article-title":"Epidermal sphingolipids: Metabolism, function, and roles in skin disorders","volume":"580","author":"Holleran","year":"2006","journal-title":"FEBS Lett."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"G\u0119gotek, A., and Skrzydlewska, E. (2022). The Role of ABC Transporters in Skin Cells Exposed to UV Radiation. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010115"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Atalay, S., G\u0119gotek, A., and Skrzydlewska, E. (2021). Protective effects of cannabidiol on the membrane proteome of UVB-irradiated keratinocytes. Antioxidants, 10.","DOI":"10.3390\/antiox10030402"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1142\/S0192415X19500940","article-title":"Anti-Apoptotic and Anti-Inflammatory Activities of Edible Fresh Water Algae Prasiola japonica in UVB-Irradiated Skin Keratinocytes","volume":"47","author":"Choi","year":"2019","journal-title":"Am. J. Chin. Med."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Da Costa, E., Melo, T., Reis, M., Domingues, P., Calado, R., Abreu, M.H., and Domingues, M.R. (2021). Polar lipids composition antioxidant and anti-inflammatory activities of the atlantic red seaweed Grateloupia turuturu. Mar. Drugs, 19.","DOI":"10.3390\/md19080414"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100831","DOI":"10.1016\/j.fbio.2020.100831","article-title":"Sargassum horneri ethanol extract ameliorates TNF-\u03b1\/IFN-\u03b3-induced inflammation in human keratinocytes and TPA-induced ear edema in mice","volume":"39","author":"Han","year":"2021","journal-title":"Food Biosci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Jayawardena, T.U., Sanjeewa, K.K.A., Wang, L., Kim, W.S., Lee, T.K., Kim, Y.T., and Jeon, Y.J. (2020). Alginic Acid from Padina boryana Abate Particulate Matter-Induced Inflammatory Responses in Keratinocytes and Dermal Fibroblasts. Molecules, 25.","DOI":"10.3390\/molecules25235746"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.jphotobiol.2017.05.042","article-title":"Protective effect of Arthrospira platensis extracts against ultraviolet B-induced cellular senescence through inhibition of DNA damage and matrix metalloproteinase-1 expression in human dermal fibroblasts","volume":"173","author":"Lee","year":"2017","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Leitner, P.D., Jakschitz, T., Gstir, R., Stuppner, S., Perkams, S., Kruus, M., Trockenbacher, A., Griesbeck, C., Bonn, G.K., and Huber, L.A. (2022). Anti-Inflammatory Extract from Soil Algae Chromochloris zofingiensis Targeting TNFR\/NF-\u03baB Signaling at Different Levels. Cells, 11.","DOI":"10.3390\/cells11091407"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1615\/JEnvironPatholToxicolOncol.2016014003","article-title":"Photoprotective effect of carpomitra costata extract against ultraviolet B-induced oxidative damage in human keratinocytes","volume":"35","author":"Zheng","year":"2016","journal-title":"J. Environ. Pathol. Toxicol. Oncol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"775","DOI":"10.4014\/jmb.1509.09067","article-title":"Extract of ettlia sp, YC001 exerts photoprotective effects against UVB irradiation in normal human dermal fibroblasts","volume":"26","author":"Lee","year":"2016","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4286","DOI":"10.1002\/jssc.201800780","article-title":"Free fatty acid profiling in marine algae extract by LC-MS\/MS and isolation as well as quantification of the \u03c9-3 fatty acid hexadeca-471013-tetraenoic acid","volume":"41","author":"Schlotterbeck","year":"2018","journal-title":"J. Sep. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1007\/s10811-012-9804-6","article-title":"Antioxidant potential of microalgae in relation to their phenolic and carotenoid content","volume":"24","author":"Goiris","year":"2012","journal-title":"J. Appl. Phycol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4355","DOI":"10.1038\/s41598-021-83455-y","article-title":"Polar lipidomic profile shows Chlorococcum amblystomatis as a promising source of value-added lipids","volume":"11","author":"Conde","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Regueiras, A., Huguet, \u00c1., Conde, T., Couto, D., Domingues, P., Domingues, M.R., Costa, A.M., Silva, J.L., da Vasconcelos, V., and Urbatzka, R. (2021). Potential Anti-Obesity Anti-Steatosis and Anti-Inflammatory Properties of Extracts from the Microalgae Chlorella vulgaris and Chlorococcum amblystomatis under Different Growth Conditions. Mar. Drugs, 20.","DOI":"10.3390\/md20010009"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"6227","DOI":"10.1021\/jf020290y","article-title":"In vitro anti-inflammatory and anti-proliferative activity of sulfolipids from the red alga Porphyridium cruentum","volume":"50","author":"Debiton","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1016\/j.celrep.2014.10.069","article-title":"Mono-and digalactosyldiacylglycerol lipids function nonredundantly to regulate systemic acquired resistance in plants","volume":"9","author":"Gao","year":"2014","journal-title":"Cell Rep."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Landon, R., Gueguen, V., Petite, H., Letourneur, D., Pavon-Djavid, G., and Anagnostou, F. (2020). Impact of Astaxanthin on Diabetes Pathogenesis and Chronic Complications. Mar. Drugs, 18.","DOI":"10.3390\/md18070357"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1080\/14756366.2020.1867121","article-title":"Thioredoxin-dependent system. Application of inhibitors","volume":"36","author":"Skrzydlewska","year":"2021","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"\u0141uczaj, W., Dobrzy\u0144ska, I., Wro\u0144ski, A., Domingues, M.R., Domingues, P., and Skrzydlewska, E. (2020). Cannabidiol-Mediated Changes to the Phospholipid Profile of UVB-Irradiated Keratinocytes from Psoriatic Patients. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21186592"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"6647222","DOI":"10.1155\/2021\/6647222","article-title":"Antioxidant and Anti-inflammatory Effect of Cannabidiol Contributes to the Decreased Lipid Peroxidation of Keratinocytes of Rat Skin Exposed to UV Radiation","volume":"2021","author":"Markowska","year":"2021","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Conde, T.A., Neves, B.F., Couto, D., Melo, T., Neves, B., Costa, M., Silva, J., Domingues, P., and Domingues, M.R. (2021). Microalgae as sustainable bio-factories of healthy lipids: Evaluating fatty acid content and antioxidant activity. Mar. Drugs, 19.","DOI":"10.3390\/md19070357"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1111\/php.12061","article-title":"Fucosterol Inhibits Matrix Metalloproteinase Expression and Promotes Type-1 Procollagen Production in UVB-induced HaCaT Cells","volume":"89","author":"Kim","year":"2013","journal-title":"Photochem. Photobiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1016\/j.jnutbio.2015.08.001","article-title":"The complexity of the Nrf2 pathway: Beyond the antioxidant response","volume":"26","author":"Huang","year":"2015","journal-title":"J. Nutr. Biochem."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"102489","DOI":"10.1016\/j.redox.2022.102489","article-title":"The molecular activity of cannabidiol in the regulation of Nrf2 system interacting with NF-\u03baB pathway under oxidative stress","volume":"57","author":"Skrzydlewska","year":"2022","journal-title":"Redox Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/s00403-015-1554-2","article-title":"The role of transcription factor Nrf2 in skin cells metabolism","volume":"307","author":"Skrzydlewska","year":"2015","journal-title":"Arch. Dermatol. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"11134","DOI":"10.1038\/s41598-020-68058-3","article-title":"A new role for anandamide: Defective link between the systemic and skin endocannabinoid systems in hypertrophic human wound healing","volume":"10","author":"Carvalho","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"111374","DOI":"10.1016\/j.biopha.2021.111374","article-title":"Nrf2 a molecular therapeutic target for Astaxanthin","volume":"137","author":"Kohandel","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jphs.2017.05.011","article-title":"Astaxanthin analogs adonixanthin and lycopene activate Nrf2 to prevent light-induced photoreceptor degeneration","volume":"134","author":"Inoue","year":"2017","journal-title":"J. Pharmacol. Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1002\/mnfr.200900159","article-title":"Hydroxytyrosol induces antioxidant\/detoxificant enzymes and Nrf2 translocation via extracellular regulated kinases and phosphatidylinositol-3-kinase\/protein kinase B pathways in HepG2 cells","volume":"54","author":"Ramos","year":"2010","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"6125","DOI":"10.3390\/md12126125","article-title":"Astaxanthin Activates Nuclear Factor Erythroid-Related Factor 2 and the Antioxidant Responsive Element (Nrf2-ARE) Pathway in the Brain after Subarachnoid Hemorrhage in Rats and Attenuates Early Brain Injury","volume":"12","author":"Wu","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"e00099-20","DOI":"10.1128\/MCB.00099-20","article-title":"The Molecular Mechanisms Regulating the KEAP1-NRF2 Pathway","volume":"40","author":"Baird","year":"2020","journal-title":"Mol. Cell Biol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.abb.2016.08.005","article-title":"Keap1 the cysteine-based mammalian intracellular sensor for electrophiles and oxidants","volume":"617","author":"Kostov","year":"2017","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1016\/j.freeradbiomed.2011.01.033","article-title":"Molecular cross-talk between the NRF2\/KEAP1 signaling pathway autophagy and apoptosis","volume":"50","author":"Kruszewski","year":"2011","journal-title":"Free Radic. Biol. Med."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"740","DOI":"10.2174\/138955708784912148","article-title":"Natural Marine Anti-inflammatory Products","volume":"8","author":"Abad","year":"2008","journal-title":"Mini-Rev. Med. Chem."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1089\/ars.2007.1807","article-title":"Therapeutic applications of bilirubin and biliverdin in transplantation","volume":"9","author":"Wang","year":"2007","journal-title":"Antioxid. Redox Signal."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"809952","DOI":"10.3389\/fcell.2021.809952","article-title":"Dissecting the crosstalk between Nrf2 and NF-\u03baB response pathways in drug-induced toxicity","volume":"9","author":"Gao","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.bbrc.2016.06.106","article-title":"Nuclear DNA damage-triggered NLRP3 inflammasome activation promotes UVB-induced inflammatory responses in human keratinocytes","volume":"477","author":"Hasegawa","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s00403-008-0893-7","article-title":"TNF-\u03b1 production in the skin","volume":"301","author":"Bashir","year":"2009","journal-title":"Arch. Dermatol. Res."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Zarkovic, N., Biernacki, M., W\u00f3jcik, P., Jarocka-Karpowicz, I., Orehovec, B., Bar\u0161i\u0107, B., Tarle, M., Kmet, M., Luk\u0161i\u0107, I., and Skrzydlewska, E. (2022). Alterations of the Antioxidant and Inflammatory Response of the Peripheral Blood Granulocytes to SARS-CoV-2 Infection in the Deceased COVID-19 Patients. Immunol. Microbiol.","DOI":"10.20944\/preprints202209.0298.v1"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1128\/MCB.25.11.4501-4513.2005","article-title":"Keap1 Regulates the Oxidation-Sensitive Shuttling of Nrf2 into and out of the Nucleus via a Crm1-Dependent Nuclear Export Mechanism","volume":"25","author":"Velichkova","year":"2005","journal-title":"Mol. Cell Biol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"13935","DOI":"10.1038\/s41598-019-50206-z","article-title":"Heterotrophy as a tool to overcome the long and costly autotrophic scale-up process for large scale production of microalgae","volume":"9","author":"Barros","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Lees","year":"1957","journal-title":"J. Biol. Chem."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.toxlet.2005.07.001","article-title":"In vitro cytotoxicity assays: Comparison of LDH neutral red MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride","volume":"160","author":"Fotakis","year":"2006","journal-title":"Toxicol. Lett."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1161\/01.HYP.0000258594.87211.6b","article-title":"Measurement of reactive oxygen species in cardiovascular studies","volume":"49","author":"Dikalov","year":"2007","journal-title":"Hypertension"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1161\/01.RES.74.6.1141","article-title":"Angiotensin II stimulates NADH and NADPH oxidase activity in cultured vascular smooth muscle cells","volume":"74","author":"Griendling","year":"1994","journal-title":"Circ. Res."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0014-5793(75)80385-1","article-title":"Malignant transformation-linked imbalance: Decreased xanthine oxidase activity in hepatomas","volume":"59","author":"Prajda","year":"1975","journal-title":"FEBS Lett."},{"key":"ref_93","first-page":"2759","article-title":"A preliminary study of the superoxide dismutase content of some human tumors","volume":"38","author":"Sykes","year":"1978","journal-title":"Cancer Res."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/0003-2697(83)90348-2","article-title":"A method for distinguishing CuZn- and Mn-containing superoxide dismutases","volume":"128","author":"Geller","year":"1983","journal-title":"Anal. Biochem."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0076-6879(84)05016-3","article-title":"Catalase in Vitro","volume":"105","author":"Aebi","year":"1984","journal-title":"Methods Enzymol."},{"key":"ref_96","first-page":"158","article-title":"Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase","volume":"70","author":"Paglia","year":"1967","journal-title":"J. Lab. Clin. Med."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1016\/S0021-9258(19)83745-6","article-title":"Hepatic Glutathione Reductase","volume":"237","author":"Mize","year":"1962","journal-title":"J. Biol. Chem."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jchromb.2005.05.015","article-title":"Capillary electrophoresis of glutathione to monitor oxidative stress and response to antioxidant treatments in an animal model","volume":"822","author":"Maeso","year":"2005","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.bbrc.2010.03.083","article-title":"Thioredoxin and thioredoxin reductase: Current research with special reference to human disease","volume":"396","author":"Holmgren","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1080\/01971520009349533","article-title":"Enzyme-linked immunosorbent assay","volume":"21","author":"Butler","year":"2000","journal-title":"J Immunoass."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ab.2016.08.009","article-title":"Simultaneous GC-MS\/MS measurement of malondialdehyde and 4-hydroxy-2-nonenal in human plasma: Effects of long-term L-arginine administration","volume":"524","author":"Tsikas","year":"2017","journal-title":"Anal. Biochem."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s00726-005-0229-2","article-title":"Kinetics of biomarkers: Biological and technical validity of isoprostanes in plasma","volume":"29","author":"Coolen","year":"2005","journal-title":"Amino Acids"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/14\/11302\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:10:30Z","timestamp":1760127030000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/14\/11302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,11]]},"references-count":102,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ijms241411302"],"URL":"https:\/\/doi.org\/10.3390\/ijms241411302","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,11]]}}}