{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:44:34Z","timestamp":1760150674342,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T00:00:00Z","timestamp":1702684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"project CICECO-Aveiro Institute of Materials","award":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"],"award-info":[{"award-number":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"]}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"],"award-info":[{"award-number":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Portuguese National NMR Network (RNRMN)","award":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"],"award-info":[{"award-number":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"]}]},{"name":"FCT\/SPQ","award":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"],"award-info":[{"award-number":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"]}]},{"name":"FCT","award":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"],"award-info":[{"award-number":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"]}]},{"name":"FCT\/MCTES","award":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"],"award-info":[{"award-number":["UIDB\/50011\/2020","UIDP\/50011\/2020","LA\/P\/0006\/2020","UIDB\/50006\/2020","UIDB\/00070\/2020","UIDP\/00070\/2020","PO-CI-01-0145-FEDER-0016786","BetterBone-2022.04286.PTDC","N-022161","SFRH\/BD\/150655\/2020","SFRH\/BD\/145920\/2019","SFRH\/BD\/ 111576\/2015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This work investigated the mechanisms of action of conventional drugs, cisplatin and oxaliplatin, and the potentially less deleterious drug Pd2Spermine (Spm) and its Pt(II) analog, against osteosarcoma MG-63 cells, using nuclear-magnetic-resonance metabolomics of the cellular lipidome. The Pt(II) chelates induced different responses, namely regarding polyunsaturated-fatty-acids (increased upon cisplatin), suggesting that cisplatin-treated cells have higher membrane fluidity\/permeability, thus facilitating cell entry and justifying higher cytotoxicity. Both conventional drugs significantly increased triglyceride levels, while Pt2Spm maintained control levels; this may reflect enhanced apoptotic behavior for conventional drugs, but not for Pt2Spm. Compared to Pt2Spm, the more cytotoxic Pd2Spm (IC50 comparable to cisplatin) induced a distinct phospholipids profile, possibly reflecting enhanced de novo biosynthesis to modulate membrane fluidity and drug-accessibility to cells, similarly to cisplatin. However, Pd2Spm differed from cisplatin in that cells had equivalent (low) levels of triglycerides as Pt2Spm, suggesting the absence\/low extent of apoptosis. Our results suggest that Pd2Spm acts on MG-63 cells mainly through adaptation of cell membrane fluidity, whereas cisplatin seems to couple a similar effect with typical signs of apoptosis. These results were discussed in articulation with reported polar metabolome adaptations, building on the insight of these drugs\u2019 mechanisms, and particularly of Pd2Spm as a possible cisplatin substitute.<\/jats:p>","DOI":"10.3390\/ijms242417556","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T03:59:24Z","timestamp":1702871964000},"page":"17556","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Impact of Conventional and Potential New Metal-Based Drugs on Lipid Metabolism in Osteosarcoma MG-63 Cells"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5813-5421","authenticated-orcid":false,"given":"Daniela S. C.","family":"Bispo","sequence":"first","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2026-9088","authenticated-orcid":false,"given":"Marlene","family":"Correia","sequence":"additional","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4841-7860","authenticated-orcid":false,"given":"Tatiana J.","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"},{"name":"Unidade de I&D Qu\u00edmica-F\u00edsica Molecular, Department of Chemistry, University of Coimbra, Rua Larga, 300-535 Coimbra, Portugal"}]},{"given":"Ana S.","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"},{"name":"Unidade de I&D Qu\u00edmica-F\u00edsica Molecular, Department of Chemistry, University of Coimbra, Rua Larga, 300-535 Coimbra, Portugal"}]},{"given":"Aliana A. N.","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1280-3321","authenticated-orcid":false,"given":"Ana L. M. Batista de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Unidade de I&D Qu\u00edmica-F\u00edsica Molecular, Department of Chemistry, University of Coimbra, Rua Larga, 300-535 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-0055","authenticated-orcid":false,"given":"Maria P. M.","family":"Marques","sequence":"additional","affiliation":[{"name":"Unidade de I&D Qu\u00edmica-F\u00edsica Molecular, Department of Chemistry, University of Coimbra, Rua Larga, 300-535 Coimbra, Portugal"},{"name":"Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3766-4364","authenticated-orcid":false,"given":"Ana M.","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Chemistry, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.4103\/0971-5851.203513","article-title":"Pediatric Osteosarcoma: An Updated Review","volume":"38","author":"Taran","year":"2017","journal-title":"Indian J. Med. Paediatr. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1200\/JCO.2014.59.4895","article-title":"Osteosarcoma: Current Treatment and a Collaborative Pathway to Success","volume":"33","author":"Isakoff","year":"2015","journal-title":"J. Clin. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.2165\/11316640-000000000-00000","article-title":"Cisplatin Overdose: Toxicities and Management","volume":"32","author":"Tsang","year":"2009","journal-title":"Drug Saf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/S1470-2045(10)70062-0","article-title":"Sequelae of Osteosarcoma Medical Therapy: A Review of Rare Acute Toxicities and Late Effects","volume":"11","author":"Janeway","year":"2010","journal-title":"Lancet Oncol."},{"key":"ref_5","first-page":"6228","article-title":"Progress in the Chemotherapeutic Treatment of Osteosarcoma","volume":"16","author":"Zhang","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/205698a0","article-title":"Inhibition of Cell Division in Escherichia Coli by Electrolysis Products from a Platinum Electrode","volume":"205","author":"Rosenberg","year":"1965","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1595\/003214079X2339098","article-title":"Cisplatin in the Treatment of Cancer: The First Metal Antitumor Drug","volume":"23","author":"Wiltshaw","year":"1979","journal-title":"Platin. Met. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1038\/nrc2167","article-title":"The Resurgence of Platinum-Based Cancer Chemotherapy","volume":"7","author":"Kelland","year":"2007","journal-title":"Nat. Rev. Cancer"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.ejphar.2014.07.025","article-title":"Cisplatin in Cancer Therapy: Molecular Mechanisms of Action","volume":"740","author":"Dasari","year":"2014","journal-title":"Eur. J. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1038\/onc.2011.384","article-title":"Molecular Mechanisms of Cisplatin Resistance","volume":"31","author":"Galluzzi","year":"2012","journal-title":"Oncogene"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6645","DOI":"10.1039\/C8DT00838H","article-title":"The Side Effects of Platinum-Based Chemotherapy Drugs: A Review for Chemists","volume":"47","author":"Oun","year":"2018","journal-title":"Dalton Trans."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1021\/cr068207j","article-title":"Direct Cellular Responses to Platinum-Induced DNA Damage","volume":"107","author":"Jung","year":"2007","journal-title":"Chem. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"102112","DOI":"10.1016\/j.ctrv.2020.102112","article-title":"Oxaliplatin Retreatment in Metastatic Colorectal Cancer: Systematic Review and Future Research Opportunities","volume":"91","author":"Mauri","year":"2020","journal-title":"Cancer Treat. Rev."},{"key":"ref_14","first-page":"e287353","article-title":"Platinum and Palladium Polyamine Complexes as Anticancer Agents: The Structural Factor","volume":"2013","author":"Marques","year":"2013","journal-title":"Int. Sch. Res. Not."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1016\/j.drudis.2019.02.012","article-title":"Anticancer Activity of Palladium-Based Complexes against Triple-Negative Breast Cancer","volume":"24","author":"Vojtek","year":"2019","journal-title":"Drug Discov. Today"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1021\/acs.jproteome.7b00035","article-title":"Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study","volume":"16","author":"Lamego","year":"2017","journal-title":"J. Proteome Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5993","DOI":"10.1002\/slct.202001361","article-title":"Cytotoxicity of Platinum and Palladium Chelates against Osteosarcoma","volume":"5","author":"Martins","year":"2020","journal-title":"ChemistrySelect"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Martins, A.S., Batista de Carvalho, A.L.M., Marques, M.P.M., and Gil, A.M. (2021). Response of Osteosarcoma Cell Metabolism to Platinum and Palladium Chelates as Potential New Drugs. Molecules, 26.","DOI":"10.3390\/molecules26164805"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1475-2867-3-5","article-title":"NMR Studies of the Relationship between the Changes of Membrane Lipids and the Cisplatin-Resistance of A549\/DDP Cells","volume":"3","author":"Huang","year":"2003","journal-title":"Cancer Cell Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"31","DOI":"10.6564\/JKMRS.2017.21.1.031","article-title":"Comparison of Metabolic Profiles of Normal and Cancer Cells in Response to Cytotoxic Agents","volume":"21","author":"Lee","year":"2017","journal-title":"J. Korean Magn. Reson. Soc."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Vermathen, M., von Tengg-Kobligk, H., Hungerb\u00fchler, M.N., Vermathen, P., and Ruprecht, N. (2021). 1H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations. Mol. Basel Switz., 26.","DOI":"10.3390\/molecules26226766"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1093\/jnci\/djh273","article-title":"Predicting Resistance or Response to Chemotherapy by Proton Magnetic Resonance Spectroscopy in Neuroblastoma","volume":"96","author":"Lindskog","year":"2004","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3493","DOI":"10.1021\/pr200114v","article-title":"In Vitro Metabonomic Study Detects Increases in UDP-GlcNAc and UDP-GalNAc, as Early Phase Markers of Cisplatin Treatment Response in Brain Tumor Cells","volume":"10","author":"Pan","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1007\/s11306-012-0483-8","article-title":"Increased Unsaturation of Lipids in Cytoplasmic Lipid Droplets in DAOY Cancer Cells in Response to Cisplatin Treatment","volume":"9","author":"Pan","year":"2013","journal-title":"Metabolomics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.jpba.2017.08.038","article-title":"Impact of Chemotherapy on Metabolic Reprogramming: Characterization of the Metabolic Profile of Breast Cancer MDA-MB-231 Cells Using 1H HR-MAS NMR Spectroscopy","volume":"146","author":"Maria","year":"2017","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1021\/acs.biochem.7b00015","article-title":"Effects of Doxorubicin, Cisplatin, and Tamoxifen on the Metabolic Profile of Human Breast Cancer MCF-7 Cells As Determined by 1H High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance","volume":"56","author":"Maria","year":"2017","journal-title":"Biochemistry"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Del Coco, L., Majellaro, M., Boccarelli, A., Cellamare, S., Altomare, C.D., and Fanizzi, F.P. (2020). Novel Antiproliferative Biphenyl Nicotinamide: NMR Metabolomic Study of Its Effect on the MCF-7 Cell in Comparison with Cisplatin and Vinblastine. Molecules, 25.","DOI":"10.3390\/molecules25153502"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Resendiz-Acevedo, K., Garc\u00eda-Aguilera, M.E., Esturau-Escofet, N., and Ruiz-Azuara, L. (2021). 1H -NMR Metabolomics Study of the Effect of Cisplatin and Casiopeina IIgly on MDA-MB-231 Breast Tumor Cells. Front. Mol. Biosci., 8.","DOI":"10.3389\/fmolb.2021.742859"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"De Castro, F., Benedetti, M., Antonaci, G., Del Coco, L., De Pascali, S.A., Muscella, A., Marsigliante, S., and Fanizzi, F.P. (2018). Response of Cisplatin Resistant Skov-3 Cells to [Pt(O,O\u2032-Acac)(\u03b3-Acac)(DMS)] Treatment Revealed by a Metabolomic 1H-NMR Study. Molecules, 23.","DOI":"10.3390\/molecules23092301"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5877","DOI":"10.1021\/pr100635n","article-title":"Nuclear Magnetic Resonance (NMR) Study of the Effect of Cisplatin on the Metabolic Profile of MG-63 Osteosarcoma Cells","volume":"9","author":"Duarte","year":"2010","journal-title":"J. Proteome Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6033","DOI":"10.1021\/pr500907d","article-title":"Metabolic Markers of MG-63 Osteosarcoma Cell Line Response to Doxorubicin and Methotrexate Treatment: Comparison to Cisplatin","volume":"13","author":"Lamego","year":"2014","journal-title":"J. Proteome Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1021\/acs.jproteome.9b00635","article-title":"1H NMR-Based Metabolic Profiles Delineate the Anticancer Effect of Vitamin C and Oxaliplatin on Hepatocellular Carcinoma Cells","volume":"19","author":"Lin","year":"2020","journal-title":"J. Proteome Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.tibtech.2004.03.007","article-title":"Metabolomics by Numbers: Acquiring and Understanding Global Metabolite Data","volume":"22","author":"Goodacre","year":"2004","journal-title":"Trends Biotechnol."},{"key":"ref_34","unstructured":"Frisch, M.J., Trucks, G.W., Schlegel, H.B., Scuseria, G.E., Robb, M.A., Cheeseman, J.R., Scalmani, G., Barone, V., Petersson, G.A., and Nakatsuji, H. (2016). Wallingford CT Gaussian 16, Revision C.01 2016, Gaussian Inc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1667\/RR0635.1","article-title":"Temporal Dynamics of 1H-NMR-Visible Metabolites during Radiation-Induced Apoptosis in MG-63 Human Osteosarcoma Spheroids","volume":"166","author":"Santini","year":"2006","journal-title":"Radiat. Res."},{"key":"ref_36","first-page":"267","article-title":"1H-NMR Evidence for a Different Response to the Same Dose (2 Gy) of Ionizing Radiation of MG-63 Human Osteosarcoma Cells and Three-Dimensional Spheroids","volume":"26","author":"Santini","year":"2006","journal-title":"Anticancer Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1667\/RR3500.1","article-title":"Increases in 1H-NMR Mobile Lipids Are Not Always Associated with Overt Apoptosis: Evidence from MG-63 Human Osteosarcoma Three-Dimensional Spheroids Exposed to a Low Dose (2 Gy) of Ionizing Radiation","volume":"165","author":"Santini","year":"2006","journal-title":"Radiat. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.chemphyslip.2007.06.213","article-title":"The Influence of Fatty Acids on Model Cholesterol\/Phospholipid Membranes","volume":"150","author":"Wydro","year":"2007","journal-title":"Chem. Phys. Lipids"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s10863-020-09846-4","article-title":"Lipid Composition of the Cancer Cell Membrane","volume":"52","author":"Szlasa","year":"2020","journal-title":"J. Bioenerg. Biomembr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"101043","DOI":"10.1016\/j.tranon.2021.101043","article-title":"Influence of Cholesterol on Cancer Progression and Therapy","volume":"14","author":"Mayengbam","year":"2021","journal-title":"Transl. Oncol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/j.ijnurstu.2012.01.015","article-title":"Effect Size Estimation: Methods and Examples","volume":"49","author":"Berben","year":"2012","journal-title":"Int. J. Nurs. Stud."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1194\/jlr.M011825","article-title":"Investigation of Adipose Tissues in Zucker Rats Using in Vivo and Ex Vivo Magnetic Resonance Spectroscopy","volume":"52","author":"Mosconi","year":"2011","journal-title":"J. Lipid Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1038\/sj.bjc.6603778","article-title":"N-(4-Iodophenyl)-N\u2032-(2-Chloroethyl)Urea as a Microtubule Disrupter: In Vitro and in Vivo Profiling of Antitumoral Activity on CT-26 Murine Colon Carcinoma Cell Line Cultured and Grafted to Mice","volume":"96","author":"Borel","year":"2007","journal-title":"Br. J. Cancer"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1002\/(SICI)1522-2675(19990707)82:7<1025::AID-HLCA1025>3.0.CO;2-1","article-title":"Palladium(II) and Platinum(II) Polyamine Complexes: X-Ray Crystal Structures of (SP-4-2)-ChloroN-[(3-Amino-\u03baN)Propyl]Propane-1,3-Diamine-\u03baN,\u03baN\u2032palladium(1+) Tetrachloropalladate (2\u2013) (2 : 1) and (R,S)-Tetrachloro[\u03bc-(Spermine)]Dipalladium(II) (=\u03bc-N,N\u2032-Bis[(3-Amino-\u03baN)Propyl]Butane-1,4-Diamine-\u03baN:\u03baN\u2032tetrachlorodipalladium)","volume":"82","author":"Codina","year":"1999","journal-title":"Helv. Chim. Acta"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6274","DOI":"10.1039\/C5NJ01088H","article-title":"Conformational Insights and Vibrational Study of a Promising Anticancer Agent: The Role of the Ligand in Pd(II)\u2013Amine Complexes","volume":"39","author":"Fiuza","year":"2015","journal-title":"New J. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1007\/s00216-010-4425-x","article-title":"Metabolite Extraction from Adherently Growing Mammalian Cells for Metabolomics Studies: Optimization of Harvesting and Extraction Protocols","volume":"399","author":"Dettmer","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2017.05.011","article-title":"Quantitative NMR Analysis of Intra- and Extracellular Metabolism of Mammalian Cells: A Tutorial","volume":"980","author":"Kostidis","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1373\/clinchem.2006.069112","article-title":"Diagnosing Inborn Errors of Lipid Metabolism with Proton Nuclear Magnetic Resonance Spectroscopy","volume":"52","author":"Oostendorp","year":"2006","journal-title":"Clin. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Bernardini, C. (2018). Duchenne Muscular Dystrophy: Methods and Protocols, Springer. Methods in Molecular Biology.","DOI":"10.1007\/978-1-4939-7374-3"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Alexandri, E., Ahmed, R., Siddiqui, H., Choudhary, M.I., Tsiafoulis, C.G., and Gerothanassis, I.P. (2017). High Resolution NMR Spectroscopy as a Structural and Analytical Tool for Unsaturated Lipids in Solution. Molecules, 22.","DOI":"10.3390\/molecules22101663"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1002\/nbm.1191","article-title":"NMR Spectroscopic Identification of Cholesterol Esters, Plasmalogen and Phenolic Glycolipids as Fingerprint Markers of Human Intracranial Tuberculomas","volume":"21","author":"Subramanian","year":"2008","journal-title":"NMR Biomed."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"D622","DOI":"10.1093\/nar\/gkab1062","article-title":"HMDB 5.0: The Human Metabolome Database for 2022","volume":"50","author":"Wishart","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1021\/ac8011544","article-title":"Recursive Segment-Wise Peak Alignment of Biological 1H NMR Spectra for Improved Metabolic Biomarker Recovery","volume":"81","author":"Veselkov","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1021\/pr060594q","article-title":"Chemometrics in Metabonomics","volume":"6","author":"Trygg","year":"2007","journal-title":"J. Proteome Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0895-4356(98)00168-1","article-title":"Increasing Physicians\u2019 Awareness of the Impact of Statistics on Research Outcomes: Comparative Power of the t-Test and Wilcoxon Rank-Sum Test in Small Samples Applied Research","volume":"52","author":"Bridge","year":"1999","journal-title":"J. Clin. Epidemiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/24\/17556\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:40:06Z","timestamp":1760132406000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/24\/17556"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,16]]},"references-count":55,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["ijms242417556"],"URL":"https:\/\/doi.org\/10.3390\/ijms242417556","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2023,12,16]]}}}