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TE technique is characterized by several stages, starting from the choice of cell culture and the more appropriate scaffold material that can adequately support and supply them with the necessary biological cues for microvessel development. The next step is to analyze the attained microvasculature, which is reliant on the available labeling and microscopy techniques to visualize the network, as well as metrics employed to characterize it. These are usually attained with the use of software, which has been cited in several works, although no clear standard procedure has been observed to promote the reproduction of the cell response analysis. The present review analyzes not only the various steps previously described in terms of the current standards for evaluation, but also surveys some of the available metrics and software used to quantify networks, along with the detection of analysis limitations and future improvements that could lead to considerable progress for angiogenesis evaluation and application in TE research.<\/jats:p>","DOI":"10.3390\/ijms242417625","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T10:04:47Z","timestamp":1702893887000},"page":"17625","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Comprehensive Look at In Vitro Angiogenesis Image Analysis Software"],"prefix":"10.3390","volume":"24","author":[{"given":"Mariana","family":"Pereira","sequence":"first","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"INEB\u2014Instituto de Engenharia Biom\u00e9dica, Universidade do Porto, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6325-1761","authenticated-orcid":false,"given":"J\u00e9ssica","family":"Pinto","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"INEB\u2014Instituto de Engenharia Biom\u00e9dica, Universidade do Porto, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5965-8630","authenticated-orcid":false,"given":"Bel\u00e9n","family":"Arteaga","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"INEB\u2014Instituto de Engenharia Biom\u00e9dica, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Faculty of Medicine, University of Granada, Parque Tecnol\u00f3gico de la Salud, Av. de la Investigaci\u00f3n 11, 18016 Granada, Spain"}]},{"given":"Ana","family":"Guerra","sequence":"additional","affiliation":[{"name":"INEGI\u2014Instituto de Ci\u00eancia e Inova\u00e7\u00e3o em Engenharia Mec\u00e2nica e Engenharia Industrial, 4200-465 Porto, Portugal"}]},{"given":"Renato Natal","family":"Jorge","sequence":"additional","affiliation":[{"name":"INEGI\u2014Instituto de Ci\u00eancia e Inova\u00e7\u00e3o em Engenharia Mec\u00e2nica e Engenharia Industrial, 4200-465 Porto, Portugal"},{"name":"LAETA\u2014Laborat\u00f3rio Associado de Energia, Transportes e Aeron\u00e1utica, Universidade do Porto, 4200-165 Porto, Portugal"},{"name":"FEUP\u2014Faculdade de Engenharia, Departamento de Engenharia Metal\u00fargica e de Materiais, Universidade do Porto, 4200-165 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1361-4605","authenticated-orcid":false,"given":"Fernando Jorge","family":"Monteiro","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"INEB\u2014Instituto de Engenharia Biom\u00e9dica, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"FEUP\u2014Faculdade de Engenharia, Departamento de Engenharia Metal\u00fargica e de Materiais, Universidade do Porto, 4200-165 Porto, Portugal"},{"name":"PCCC\u2014Porto Comprehensive Cancer Center, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3844-1667","authenticated-orcid":false,"given":"Christiane Laranjo","family":"Salgado","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"INEB\u2014Instituto de Engenharia Biom\u00e9dica, Universidade do Porto, 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,18]]},"reference":[{"key":"ref_1","unstructured":"Wahid, F., Khan, T., Hussain, Z., and Ullah, H. 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