{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T12:17:12Z","timestamp":1772799432311,"version":"3.50.1"},"reference-count":72,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T00:00:00Z","timestamp":1715040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016991","name":"Universidad de Guadalajara","doi-asserted-by":"publisher","award":["Fortalecimiento IECD 260428-2021\/266214-2022\/271879-2023"],"award-info":[{"award-number":["Fortalecimiento IECD 260428-2021\/266214-2022\/271879-2023"]}],"id":[{"id":"10.13039\/100016991","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016991","name":"Universidad de Guadalajara","doi-asserted-by":"publisher","award":["PIN-271723-2023"],"award-info":[{"award-number":["PIN-271723-2023"]}],"id":[{"id":"10.13039\/100016991","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016991","name":"Universidad de Guadalajara","doi-asserted-by":"publisher","award":["PTDC\/OCE-ETA\/4836\/2021"],"award-info":[{"award-number":["PTDC\/OCE-ETA\/4836\/2021"]}],"id":[{"id":"10.13039\/100016991","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016991","name":"Universidad de Guadalajara","doi-asserted-by":"publisher","award":["966140"],"award-info":[{"award-number":["966140"]}],"id":[{"id":"10.13039\/100016991","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (Lisbon, Portugal)","award":["Fortalecimiento IECD 260428-2021\/266214-2022\/271879-2023"],"award-info":[{"award-number":["Fortalecimiento IECD 260428-2021\/266214-2022\/271879-2023"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (Lisbon, Portugal)","award":["PIN-271723-2023"],"award-info":[{"award-number":["PIN-271723-2023"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (Lisbon, Portugal)","award":["PTDC\/OCE-ETA\/4836\/2021"],"award-info":[{"award-number":["PTDC\/OCE-ETA\/4836\/2021"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (Lisbon, Portugal)","award":["966140"],"award-info":[{"award-number":["966140"]}]},{"name":"CONACYT, now CONAHCYT (Doctorado en Ciencias en Biolog\u00eda Molecular en Medicina, CUCS, UdeG, doctoral scholarship","award":["Fortalecimiento IECD 260428-2021\/266214-2022\/271879-2023"],"award-info":[{"award-number":["Fortalecimiento IECD 260428-2021\/266214-2022\/271879-2023"]}]},{"name":"CONACYT, now CONAHCYT (Doctorado en Ciencias en Biolog\u00eda Molecular en Medicina, CUCS, UdeG, doctoral scholarship","award":["PIN-271723-2023"],"award-info":[{"award-number":["PIN-271723-2023"]}]},{"name":"CONACYT, now CONAHCYT (Doctorado en Ciencias en Biolog\u00eda Molecular en Medicina, CUCS, UdeG, doctoral scholarship","award":["PTDC\/OCE-ETA\/4836\/2021"],"award-info":[{"award-number":["PTDC\/OCE-ETA\/4836\/2021"]}]},{"name":"CONACYT, now CONAHCYT (Doctorado en Ciencias en Biolog\u00eda Molecular en Medicina, CUCS, UdeG, doctoral scholarship","award":["966140"],"award-info":[{"award-number":["966140"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic obstructive pulmonary disease (COPD) is comprised of histopathological alterations such as pulmonary emphysema and peribronchial fibrosis. Matrix metalloproteinase 9 (MMP-9) is one of the key enzymes involved in both types of tissue remodeling during the development of lung damage. In recent studies, it was demonstrated that deflamin, a protein component extracted from Lupinus albus, markedly inhibits the catalytic activity of MMP-9 in experimental models of colon adenocarcinoma and ulcerative colitis. Therefore, in the present study, we investigated for the first time the biological effect of deflamin in a murine COPD model induced by chronic exposure to ozone. Ozone exposure was carried out in C57BL\/6 mice twice a week for six weeks for 3 h each time, and the treated group was orally administered deflamin (20 mg\/kg body weight) after each ozone exposure. The histological results showed that deflamin attenuated pulmonary emphysema and peribronchial fibrosis, as evidenced by H&amp;E and Masson\u2019s trichrome staining. Furthermore, deflamin administration significantly decreased MMP-9 activity, as assessed by fluorogenic substrate assay and gelatin zymography. Interestingly, bioinformatic analysis reveals a plausible interaction between deflamin and MMP-9. Collectively, our findings demonstrate the therapeutic potential of deflamin in a COPD murine model, and suggest that the attenuation of the development of lung tissue damage occurs by deflamin-regulated MMP-9 catalytic activity.<\/jats:p>","DOI":"10.3390\/ijms25105063","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T06:53:27Z","timestamp":1715064807000},"page":"5063","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8090-6966","authenticated-orcid":false,"given":"Elia Ana","family":"Baltazar-Garc\u00eda","sequence":"first","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Enfermedades Cr\u00f3nico-Degenerativas, Instituto Transdisciplinar de Investigaci\u00f3n e Innovaci\u00f3n en Salud, Departamento de Biolog\u00eda Molecular y Gen\u00f3mica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Puerta peatonal 7, Col. Independencia, Guadalajara 44350, Jalisco, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9112-5153","authenticated-orcid":false,"given":"Belinda","family":"Vargas-Guerrero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Enfermedades Cr\u00f3nico-Degenerativas, Instituto Transdisciplinar de Investigaci\u00f3n e Innovaci\u00f3n en Salud, Departamento de Biolog\u00eda Molecular y Gen\u00f3mica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Puerta peatonal 7, Col. Independencia, Guadalajara 44350, Jalisco, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8251-6286","authenticated-orcid":false,"given":"Ana","family":"Lima","sequence":"additional","affiliation":[{"name":"CECAV\u2014Centro de Ci\u00eancia Animal e Veterin\u00e1ria, Faculty of Veterinary Medicine, Lus\u00f3fona University, Campo Grande, 376, 1749-024 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5027-7564","authenticated-orcid":false,"given":"Ricardo","family":"Boavida Ferreira","sequence":"additional","affiliation":[{"name":"LEAF\u2014Landscape Environment Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9772-124X","authenticated-orcid":false,"given":"Mar\u00eda Luisa","family":"Mendoza-Maga\u00f1a","sequence":"additional","affiliation":[{"name":"Laboratorio de Neurofisiolog\u00eda, Departamento de Fisiolog\u00eda, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Puerta peatonal 7, Col. Independencia, Guadalajara 44350, Jalisco, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8346-2785","authenticated-orcid":false,"given":"Mario Alberto","family":"Ram\u00edrez-Herrera","sequence":"additional","affiliation":[{"name":"Laboratorio de Neurofisiolog\u00eda, Departamento de Fisiolog\u00eda, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Puerta peatonal 7, Col. Independencia, Guadalajara 44350, Jalisco, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4243-4224","authenticated-orcid":false,"given":"Tonatiuh Abimael","family":"Baltazar-D\u00edaz","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Enfermedades Cr\u00f3nico-Degenerativas, Instituto Transdisciplinar de Investigaci\u00f3n e Innovaci\u00f3n en Salud, Departamento de Biolog\u00eda Molecular y Gen\u00f3mica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Puerta peatonal 7, Col. Independencia, Guadalajara 44350, Jalisco, Mexico"}]},{"given":"Jos\u00e9 Alfredo","family":"Dom\u00ednguez-Rosales","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Enfermedades Cr\u00f3nico-Degenerativas, Instituto Transdisciplinar de Investigaci\u00f3n e Innovaci\u00f3n en Salud, Departamento de Biolog\u00eda Molecular y Gen\u00f3mica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Puerta peatonal 7, Col. Independencia, Guadalajara 44350, Jalisco, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9556-8955","authenticated-orcid":false,"given":"Adriana Mar\u00eda","family":"Salazar-Montes","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Enfermedades Cr\u00f3nico-Degenerativas, Instituto Transdisciplinar de Investigaci\u00f3n e Innovaci\u00f3n en Salud, Departamento de Biolog\u00eda Molecular y Gen\u00f3mica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Puerta peatonal 7, Col. Independencia, Guadalajara 44350, Jalisco, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9851-8961","authenticated-orcid":false,"given":"Carmen Magdalena","family":"Gurrola-D\u00edaz","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Enfermedades Cr\u00f3nico-Degenerativas, Instituto Transdisciplinar de Investigaci\u00f3n e Innovaci\u00f3n en Salud, Departamento de Biolog\u00eda Molecular y Gen\u00f3mica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Puerta peatonal 7, Col. Independencia, Guadalajara 44350, Jalisco, Mexico"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,7]]},"reference":[{"key":"ref_1","unstructured":"(2023, December 05). Global Initiative for Chronic Obstructive Lung Disease Global Strategy for Diagnosis, Management, and Prevention of Chronic Obstructive Pulmonary Disease (2023 Report). 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