{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T02:51:17Z","timestamp":1774579877203,"version":"3.50.1"},"reference-count":88,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union Next-Generation EU (PIANO NAZIONALE DI RIPRESA E RESILIENZA (PNRR)","award":["CN000022"],"award-info":[{"award-number":["CN000022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Understanding the responses of olive trees to drought stress is crucial for improving cultivation and developing drought-tolerant varieties. Water transport and storage within the plant is a key factor in drought-tolerance strategies. Water management can be based on a variety of factors such as stomatal control, osmoprotectant molecules, proteins and wood properties. The aim of the study was to evaluate the water management strategy under drought stress from an anatomical and biochemical point of view in three young Italian olive cultivars (Giarraffa, Leccino and Maurino) previously distinguished for their physiological and metabolomic responses. For each cultivar, 15 individuals in pots were exposed or not to 28 days of water withholding. Every 7 days, the content of sugars (including mannitol), proline, aquaporins, osmotins, and dehydrins, in leaves and stems, as well as the chemical and anatomical characteristics of the wood of the three cultivars, were analyzed. \u2018Giarraffa\u2019 reduced glucose levels and increased mannitol production, while \u2018Leccino\u2019 accumulated more proline. Both \u2018Leccino\u2019 and \u2018Maurino\u2019 increased sucrose and aquaporin levels, possibly due to their ability to remove embolisms. \u2018Maurino\u2019 and \u2018Leccino\u2019 accumulated more dehydrins and osmotins. While neither genotype nor stress affected wood chemistry, \u2018Maurino\u2019 had a higher vessel-to-xylem area ratio and a larger hydraulic diameter, which allows it to maintain a high transpiration rate but may make it more susceptible to cavitation. The results emphasized the need for an integrated approach, highlighting the importance of the relative timing and sequence of each parameter analyzed, allowing, overall, to define a \u201cstrategy\u201d rather than a \u201cresponse\u201d to drought of each cultivar.<\/jats:p>","DOI":"10.3390\/ijms252011059","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T08:09:08Z","timestamp":1728979748000},"page":"11059","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Unravelling Different Water Management Strategies in Three Olive Cultivars: The Role of Osmoprotectants, Proteins, and Wood Properties"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1589-9074","authenticated-orcid":false,"given":"Sara","family":"Parri","sequence":"first","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"}]},{"given":"Claudia","family":"Faleri","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"}]},{"given":"Marco","family":"Romi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3040-6787","authenticated-orcid":false,"given":"Jos\u00e9 C.","family":"del R\u00edo","sequence":"additional","affiliation":[{"name":"Instituto de Recursos Naturales y Agrobiolog\u00eda de Sevilla, CSIC, Reina Mercedes 10, E-41012 Seville, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2728-7331","authenticated-orcid":false,"given":"Jorge","family":"Rencoret","sequence":"additional","affiliation":[{"name":"Instituto de Recursos Naturales y Agrobiolog\u00eda de Sevilla, CSIC, Reina Mercedes 10, E-41012 Seville, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3083-6218","authenticated-orcid":false,"given":"Maria Celeste Pereira","family":"Dias","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"given":"Sara","family":"Anichini","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"},{"name":"Department of Agriculture, Food, Environment and Forestry, University of Florence, Piazzale delle Cascine, 18, 50144 Firenze, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-6013","authenticated-orcid":false,"given":"Claudio","family":"Cantini","sequence":"additional","affiliation":[{"name":"Institute for BioEconomy (IBE), National Research Council (CNR), Strada Provinciale Aurelia Vecchia 49, 58022 Follonica, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4511-3557","authenticated-orcid":false,"given":"Giampiero","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"ref_1","unstructured":"Ali, E., Cramer, W., Carnicer, J., Georgopoulou, E., Hilmi, N.J.M., Le Cozannet, G., and Lionello, P. 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