{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T09:37:09Z","timestamp":1776505029390,"version":"3.51.2"},"reference-count":113,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,24]],"date-time":"2024-11-24T00:00:00Z","timestamp":1732406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["24-43-10005"],"award-info":[{"award-number":["24-43-10005"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["B23RNFM-041"],"award-info":[{"award-number":["B23RNFM-041"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"name":"BRFFR","award":["24-43-10005"],"award-info":[{"award-number":["24-43-10005"]}]},{"name":"BRFFR","award":["B23RNFM-041"],"award-info":[{"award-number":["B23RNFM-041"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cancer is one of the most common diseases in developed countries. Recently, gene therapy has emerged as a promising approach to cancer treatment and has already entered clinical practice worldwide. RNA interference-based therapy is a promising method for cancer treatment. However, there are a number of limitations that require vectors to deliver therapeutic nucleic acids to target tissues and organs. Active research is currently underway to find highly effective, low-toxic nanomaterials capable of acting as nanocarriers. In this study, we demonstrated for the first time the ability of symmetrical polyamidoamine dendronized thiacalix[4]arenes (PAMAM-calix-dendrimers) to form stable positively charged complexes with siRNAs, protect them from enzymatic degradation, and efficiently deliver gene material to HeLa cells. A distinctive feature of PAMAM-calix-dendrimers was the unusual decrease in hemo- and cytotoxicity with increasing generation, while these compounds did not cause toxic effects at concentrations required for siRNA binding and delivery. A comparative analysis of the efficiency of complex formation of PAMAM-calix-dendrimers and classical PAMAM dendrimers with siRNAs was also performed. The findings may facilitate the creation of novel unique gene delivery systems for cancer nanomedicine development.<\/jats:p>","DOI":"10.3390\/ijms252312614","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T03:59:01Z","timestamp":1732507141000},"page":"12614","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Non-Viral Systems Based on PAMAM-Calix-Dendrimers for Regulatory siRNA Delivery into Cancer Cells"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1924-8028","authenticated-orcid":false,"given":"Pavel","family":"Padnya","sequence":"first","affiliation":[{"name":"A.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4950-8078","authenticated-orcid":false,"given":"Igor","family":"Shiabiev","sequence":"additional","affiliation":[{"name":"A.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, Russia"}]},{"given":"Dmitry","family":"Pysin","sequence":"additional","affiliation":[{"name":"A.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8473-5327","authenticated-orcid":false,"given":"Tatiana","family":"Gerasimova","sequence":"additional","affiliation":[{"name":"Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center, Russian Academy of Sciences, 8 Arbuzov Street, 420088 Kazan, Russia"}]},{"given":"Bahdan","family":"Ranishenka","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Cell Engineering of NASB, 27 Akademicheskaya St., 220072 Minsk, Belarus"}]},{"given":"Alesia","family":"Stanavaya","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Cell Engineering of NASB, 27 Akademicheskaya St., 220072 Minsk, Belarus"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8864-7076","authenticated-orcid":false,"given":"Viktar","family":"Abashkin","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Cell Engineering of NASB, 27 Akademicheskaya St., 220072 Minsk, Belarus"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1414-4465","authenticated-orcid":false,"given":"Dzmitry","family":"Shcharbin","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Cell Engineering of NASB, 27 Akademicheskaya St., 220072 Minsk, Belarus"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6785-6645","authenticated-orcid":false,"given":"Xiangyang","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China"},{"name":"CQM\u2014Centro de Qu\u00edmica da Madeira, Universidade da Madeira, Campus Universit\u00e1rio da Penteada, 9020-105 Funchal, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1065-0854","authenticated-orcid":false,"given":"Mingwu","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4540-2364","authenticated-orcid":false,"given":"Anastasia","family":"Nazarova","sequence":"additional","affiliation":[{"name":"A.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3019-7866","authenticated-orcid":false,"given":"Ivan","family":"Stoikov","sequence":"additional","affiliation":[{"name":"A.M. Butlerov Chemistry Institute, Kazan Federal University, 18 Kremlyovskaya Str., 420008 Kazan, Russia"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,24]]},"reference":[{"key":"ref_1","unstructured":"(2024, October 25). Cancer. Available online: https:\/\/www.who.int\/health-topics\/cancer."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4992","DOI":"10.1158\/1078-0432.CCR-16-3066","article-title":"Immuno-Oncology Clinical Trial Design: Limitations, Challenges, and Opportunities","volume":"23","author":"Baik","year":"2017","journal-title":"Clin. 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