{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T12:32:04Z","timestamp":1776429124542,"version":"3.51.2"},"reference-count":48,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T00:00:00Z","timestamp":1750896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017014","name":"CINTESIS, R&amp;D Unit","doi-asserted-by":"publisher","award":["UIDB\/4255\/2020"],"award-info":[{"award-number":["UIDB\/4255\/2020"]}],"id":[{"id":"10.13039\/501100017014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017014","name":"CINTESIS, R&amp;D Unit","doi-asserted-by":"publisher","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}],"id":[{"id":"10.13039\/501100017014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017014","name":"CINTESIS, R&amp;D Unit","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}],"id":[{"id":"10.13039\/501100017014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017014","name":"CINTESIS, R&amp;D Unit","doi-asserted-by":"publisher","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}],"id":[{"id":"10.13039\/501100017014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017014","name":"CINTESIS, R&amp;D Unit","doi-asserted-by":"publisher","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}],"id":[{"id":"10.13039\/501100017014","id-type":"DOI","asserted-by":"publisher"}]},{"name":"scope of the project RISE, Associated Laboratory","award":["UIDB\/4255\/2020"],"award-info":[{"award-number":["UIDB\/4255\/2020"]}]},{"name":"scope of the project RISE, Associated Laboratory","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}]},{"name":"scope of the project RISE, Associated Laboratory","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}]},{"name":"scope of the project RISE, Associated Laboratory","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}]},{"name":"scope of the project RISE, Associated Laboratory","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences\u2014UCIBIO","award":["UIDB\/4255\/2020"],"award-info":[{"award-number":["UIDB\/4255\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences\u2014UCIBIO","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences\u2014UCIBIO","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences\u2014UCIBIO","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences\u2014UCIBIO","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy\u2014i4HB","award":["UIDB\/4255\/2020"],"award-info":[{"award-number":["UIDB\/4255\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy\u2014i4HB","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy\u2014i4HB","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy\u2014i4HB","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy\u2014i4HB","award":["LA\/P\/0053\/2020"],"award-info":[{"award-number":["LA\/P\/0053\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Delta-9-tetrahydrocannabinol (THC) is a psychoactive element of Cannabis sativa and affects the human cannabinoid system through its receptors, CB1R and CB2R. CB1R was found in several brain areas, including the hippocampal formation (HF), and it is responsible for most THC side effects. We investigated THC\u2019s effects in the HF of female Wistar rats to assess changes in its neurotransmission. Female Wister rats (n = 20) were gonadectomized under anesthesia at 8 weeks old. Afterwards, they received estradiol benzoate (EB) and\/or THC. Immunohistochemistry was performed to assess the expression of the cholinergic receptor alpha 7 subunit (CHRNA7), the vesicular acetylcholine transporter (VAChT), the vesicular glutamate transporter (VGLUT), the gamma-aminobutyric acid type A receptor (GABRA), the CB1 receptor, and estradiol receptor alpha (EB\u03b1). In the HF, the expression of CHRNA7 was increased by EB and by THC in the Oil groups but decreased by THC in the EB groups. The same is true for VGLUT expression in the DG and hilum and for GABRA expression in the hilum. The expression of VAChT and CB1 is reduced by EB, while the concomitant administration of THC increases it. GAD expression is reduced by EB administration in CA1, CA3, and DG. Our results may help with decision-making regarding the prescription of low doses of THC as a therapeutical approach.<\/jats:p>","DOI":"10.3390\/ijms26136144","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T06:55:13Z","timestamp":1750920913000},"page":"6144","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exogenous Administration of Delta-9-Tetrahydrocannabinol Affects Adult Hippocampal Neurotransmission in Female Wistar Rats"],"prefix":"10.3390","volume":"26","author":[{"given":"Ana M.","family":"Neves","sequence":"first","affiliation":[{"name":"Department of Biomedicine, Unit of Anatomy, Faculty of Medicine of the University of Porto (FMUP), Alameda Professor Hernani Monteiro, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6762-4534","authenticated-orcid":false,"given":"Sandra","family":"Leal","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, University Institute of Health Sciences\u2014CESPU, 4585-116 Gandra, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, University Institute of Health Sciences\u2014CESPU (1HTOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8873-5591","authenticated-orcid":false,"given":"Bruno M.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, Faculty of Pharmacy of the University of Porto (FFUP), R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5078-980X","authenticated-orcid":false,"given":"Susana I.","family":"S\u00e1","sequence":"additional","affiliation":[{"name":"RISE\u2014HEALTH, Department of Biomedicine, Unit of Anatomy, Faculty of Medicine of the University of Porto (FMUP), Alameda Professor Hernani Monteiro, 4200-319 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.jep.2018.09.004","article-title":"Cannabis sativa: A comprehensive ethnopharmacological review of a medicinal plant with a long history","volume":"227","author":"Bonini","year":"2018","journal-title":"J. Ethnopharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/npp.2017.140","article-title":"Sex-Dependent Effects of Cannabis and Cannabinoids: A Translational Perspective","volume":"43","author":"Cooper","year":"2018","journal-title":"Neuropsychopharmacology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1098\/rstb.2011.0381","article-title":"Targeting the endocannabinoid system with cannabinoid receptor agonists: Pharmacological strategies and therapeutic possibilities","volume":"367","author":"Pertwee","year":"2012","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Blebea, N.M., Pricopie, A.I., Vlad, R.A., and Hancu, G. (2024). Phytocannabinoids: Exploring Pharmacological Profiles and Their Impact on Therapeutical Use. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25084204"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0301-0082(02)00080-1","article-title":"Retrograde signaling in the regulation of synaptic transmission: Focus on endocannabinoids","volume":"68","author":"Alger","year":"2002","journal-title":"Prog. Neurobiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.biopsych.2015.07.028","article-title":"An Introduction to the Endogenous Cannabinoid System","volume":"79","author":"Lu","year":"2016","journal-title":"Biol. Psychiatry"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1038\/npp.2009.31","article-title":"Hippocampal CB(1) receptors mediate the memory impairing effects of Delta(9)-tetrahydrocannabinol","volume":"34","author":"Wise","year":"2009","journal-title":"Neuropsychopharmacology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1016\/j.cell.2013.10.042","article-title":"\u03949-THC-caused synaptic and memory impairments are mediated through COX-2 signaling","volume":"155","author":"Chen","year":"2013","journal-title":"Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1001\/jamapsychiatry.2015.3278","article-title":"Effects of Cannabis Use on Human Behavior, Including Cognition, Motivation, and Psychosis: A Review","volume":"73","author":"Volkow","year":"2016","journal-title":"JAMA Psychiatry"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1159\/000360925","article-title":"Anatomy of the hippocampal formation","volume":"34","author":"Schultz","year":"2014","journal-title":"Front. Neurol. Neurosci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"239","DOI":"10.4103\/0972-2327.104323","article-title":"Hippocampus in health and disease: An overview","volume":"15","author":"Anand","year":"2012","journal-title":"Ann. Indian. Acad. Neurol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1093\/schbul\/sbs062","article-title":"Glutamate dysfunction in hippocampus: Relevance of dentate gyrus and CA3 signaling","volume":"38","author":"Tamminga","year":"2012","journal-title":"Schizophr. Bull."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/jnc.14052","article-title":"Cholinergic modulation of the hippocampal region and memory function","volume":"142","author":"Haam","year":"2017","journal-title":"J. Neurochem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Dolfen, N., Veldman, M.P., Gann, M.A., von Leupoldt, A., Puts, N.A.J., Edden, R.A.E., Mikkelsen, M., Swinnen, S., Schwabe, L., and Albouy, G. (2021). A role for GABA in the modulation of striatal and hippocampal systems under stress. Commun. Biol., 4.","DOI":"10.1038\/s42003-021-02535-x"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.neubiorev.2016.03.010","article-title":"Effect of cannabis on glutamate signalling in the brain: A systematic review of human and animal evidence","volume":"64","author":"Colizzi","year":"2016","journal-title":"Neurosci. Biobehav. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1101\/lm.1893710","article-title":"Cannabinoid and cholinergic systems interact during performance of a short-term memory task in the rat","volume":"17","author":"Goonawardena","year":"2010","journal-title":"Learn. Mem."},{"key":"ref_17","unstructured":"Preedy, V.R. (2017). Chapter e14\u2014New Ethological and Morphological Perspectives for the Investigation of Panicolytic-Like Effects of Cannabidiol. Handbook of Cannabis and Related Pathologies, Academic Press."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1089\/can.2016.0010","article-title":"Sex Differences in Cannabis Use and Effects: A Cross-Sectional Survey of Cannabis Users","volume":"1","author":"Cuttler","year":"2016","journal-title":"Cannabis Cannabinoid Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1210\/en.2011-1643","article-title":"Minireview: Endocannabinoids and gonadal hormones: Bidirectional interactions in physiology and behavior","volume":"153","author":"Gorzalka","year":"2012","journal-title":"Endocrinology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1016\/S0024-3205(01)01539-9","article-title":"Endogenous cannabinoids: Metabolism and their role in reproduction","volume":"70","author":"Habayeb","year":"2002","journal-title":"Life Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1097\/MED.0000000000000686","article-title":"Effects of marijuana on reproductive health: Preconception and gestational effects","volume":"28","author":"Ryan","year":"2021","journal-title":"Curr. Opin. Endocrinol. Diabetes Obes."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/s12974-023-02768-z","article-title":"\u03b17 Nicotinic acetylcholine receptor: A key receptor in the cholinergic anti-inflammatory pathway exerting an antidepressant effect","volume":"20","author":"Liu","year":"2023","journal-title":"J. Neuroinflamm."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"509","DOI":"10.21037\/atm-21-273","article-title":"Cellular responses and functions of \u03b17 nicotinic acetylcholine receptor activation in the brain: A narrative review","volume":"9","author":"Xu","year":"2021","journal-title":"Ann. Transl. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5238","DOI":"10.1128\/MCB.00245-09","article-title":"The vesicular acetylcholine transporter is required for neuromuscular development and function","volume":"29","author":"Lima","year":"2009","journal-title":"Mol. Cell. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10254-003-0005-1","article-title":"Nicotinic acetylcholine receptors: From structure to brain function","volume":"147","author":"Hogg","year":"2003","journal-title":"Rev. Physiol. Biochem. Pharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"115455","DOI":"10.1016\/j.bbr.2025.115455","article-title":"Standardized extract of Ginkgo biloba induced memory consolidation in female mice with hypofunction of vesicular acetylcholine transporter","volume":"482","author":"Muratori","year":"2025","journal-title":"Behav. Brain Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.yhbeh.2015.06.022","article-title":"Estrogen-cholinergic interactions: Implications for cognitive aging","volume":"74","author":"Newhouse","year":"2015","journal-title":"Horm. Behav."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Yoon, E.J., Choi, Y., and Park, D. (2022). Improvement of Cognitive Function in Ovariectomized Rats by Human Neural Stem Cells Overexpressing Choline Acetyltransferase via Secretion of NGF and BDNF. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23105560"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1007\/s40520-019-01359-4","article-title":"Acetylcholinesterase inhibitors targeting the cholinergic anti-inflammatory pathway: A new therapeutic perspective in aging-related disorders","volume":"33","author":"Benfante","year":"2021","journal-title":"Aging Clin. Exp. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Piovesana, R., Salazar Intriago, M.S., Dini, L., and Tata, A.M. (2021). Cholinergic Modulation of Neuroinflammation: Focus on \u03b17 Nicotinic Receptor. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094912"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4147","DOI":"10.1113\/jphysiol.2014.273896","article-title":"Nicotinic ACh receptors in the hippocampal circuit; functional expression and role in synaptic plasticity","volume":"592","author":"Yakel","year":"2014","journal-title":"J. Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.pnpbp.2009.03.039","article-title":"The septo-hippocampal system, learning and recovery of function","volume":"33","author":"Niewiadomska","year":"2009","journal-title":"Prog. Neuropsychopharmacol. Biol. Psychiatry"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"17651","DOI":"10.1073\/pnas.1215381109","article-title":"Elimination of the vesicular acetylcholine transporter in the forebrain causes hyperactivity and deficits in spatial memory and long-term potentiation","volume":"109","author":"Martyn","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1111\/ejn.15939","article-title":"The effects of delta-9-tetrahydrocannabinol administration in the regulation of female rat sociosexual behaviour","volume":"57","author":"Costa","year":"2023","journal-title":"Eur. J. Neurosci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"\u00c5gmo, A., and Laan, E. (2022). Sexual incentive motivation, sexual behavior, and general arousal: Do rats and humans tell the same story?. Neurosci. Biobehav. Rev., 135.","DOI":"10.1016\/j.neubiorev.2022.104595"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.bbi.2025.04.030","article-title":"Beneficial and adverse effects of THC on cognition in the HIV-1 transgenic rat model: Importance of exploring task- and sex-dependent outcomes","volume":"128","author":"Ayoub","year":"2025","journal-title":"Brain Behav. Immun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1111\/jnc.13872","article-title":"Hippocampal GABAergic transmission: A new target for adenosine control of excitability","volume":"139","author":"Rombo","year":"2016","journal-title":"J. Neurochem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1177\/1535370221989263","article-title":"Influence of glutamate and GABA transport on brain excitatory\/inhibitory balance","volume":"246","author":"Sears","year":"2021","journal-title":"Exp. Biol. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1016\/j.neubiorev.2005.12.002","article-title":"Cannabinoids and prefrontal cortical function: Insights from preclinical studies","volume":"30","author":"Egerton","year":"2006","journal-title":"Neurosci. Biobehav. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1523\/JNEUROSCI.21-07-02425.2001","article-title":"Agonist-induced internalization and trafficking of cannabinoid CB1 receptors in hippocampal neurons","volume":"21","author":"Coutts","year":"2001","journal-title":"J. Neurosci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1046\/j.1471-4159.1999.0730493.x","article-title":"Internalization and recycling of the CB1 cannabinoid receptor","volume":"73","author":"Hsieh","year":"1999","journal-title":"J. Neurochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.3892\/ijmm.2016.2779","article-title":"Overlapping molecular pathways between cannabinoid receptors type 1 and 2 and estrogens\/androgens on the periphery and their involvement in the pathogenesis of common diseases (Review)","volume":"38","author":"Hojnik","year":"2016","journal-title":"Int. J. Mol. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1590\/S0102-67202012000100011","article-title":"Rat\u2019s age versus human\u2019s age: What is the relationship?","volume":"25","author":"Andreollo","year":"2012","journal-title":"Arq. Bras. Cir. Dig."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2477","DOI":"10.1111\/bcp.13710","article-title":"The pharmacokinetics and the pharmacodynamics of cannabinoids","volume":"84","author":"Lucas","year":"2018","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1080\/01616412.2016.1203124","article-title":"Regulation of NPY and \u03b1-MSH expression by estradiol in the arcuate nucleus of Wistar female rats: A stereological study","volume":"38","author":"Leal","year":"2016","journal-title":"Neurol. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1089\/can.2022.0256","article-title":"Low-Dose \u03949-THC Produces Antinociception in Female, But Not Male Rats","volume":"8","author":"Morgan","year":"2023","journal-title":"Cannabis Cannabinoid Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.brainres.2014.07.041","article-title":"Effects of sex steroids and estrogen receptor agonists on the expression of estrogen receptor alpha in the principal division of the bed nucleus of the stria terminalis of female rats","volume":"1582","author":"Leite","year":"2014","journal-title":"Brain Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.neuroscience.2015.09.030","article-title":"Effects of gonadal steroids and of estrogen receptor agonists on the expression of estrogen receptor alpha in the medial preoptic nucleus of female rats","volume":"310","author":"Martins","year":"2015","journal-title":"Neuroscience"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/13\/6144\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:59:06Z","timestamp":1760032746000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/13\/6144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,26]]},"references-count":48,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["ijms26136144"],"URL":"https:\/\/doi.org\/10.3390\/ijms26136144","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,26]]}}}