{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:32:09Z","timestamp":1775694729455,"version":"3.50.1"},"reference-count":80,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P.","doi-asserted-by":"publisher","award":["2024.03583.BDANA"],"award-info":[{"award-number":["2024.03583.BDANA"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P.","doi-asserted-by":"publisher","award":["UID\/00276\/2025"],"award-info":[{"award-number":["UID\/00276\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P.","doi-asserted-by":"publisher","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\/Minist\u00e9rio da Educa\u00e7\u00e3o, Ci\u00eancia e Inova\u00e7\u00e3o","award":["UID\/04462"],"award-info":[{"award-number":["UID\/04462"]}]},{"DOI":"10.13039\/501100001871","name":"Associate Laboratory LS4FUTURE","doi-asserted-by":"publisher","award":["LA\/P\/0087\/2020"],"award-info":[{"award-number":["LA\/P\/0087\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Enterococcus spp. are opportunistic pathogens and commensals in humans and animals and are widely used as indicators of bacterial exchange, providing insights into antimicrobial resistance (AMR) and virulence dissemination within the One Health continuum. Enterococcus from healthy companion animals and their tutors were characterized to compare AMR profiles and virulence traits between hosts and within households in Lisbon, Portugal. Fecal samples (n = 45) were collected from 17 animals and 11 tutors. Enterococci were recovered from selective media, subjected to random amplified polymorphic DNA PCR (RAPD-PCR) and species identification, tested for antimicrobial susceptibility, and screened for virulence traits. Among animal isolates, 61% were Enterococcus faecalis, 29% E. faecium, and 10% E. hirae, whereas human enterococci comprised 52% E. faecalis, 35% E. faecium, 8% E. hirae, and 4% other species. Erythromycin resistance was identical in both groups (29%; Chi-squared test, p = 0.99). Ampicillin resistance was detected in all animal samples but was absent in human samples, whereas tetracycline and rifampicin resistance showed moderate host-specific patterns. Hemolytic activity was detected in 16% of animal and 31% of human isolates, all cylA-positive. Significant associations were observed between host origin and resistance to ampicillin and rifampicin, and between species and resistance to erythromycin and tetracycline. These findings suggest that companion animals can harbor, and potentially disseminate, AMR and virulence traits, reinforcing the need for One Health surveillance.<\/jats:p>","DOI":"10.3390\/ijms27020654","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T08:09:06Z","timestamp":1767946146000},"page":"654","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["One Health Insights into Enterococcus: Antimicrobial Resistance and Virulence in Companion Animals and Their Tutors"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8944-3235","authenticated-orcid":false,"given":"Joana","family":"Monteiro Marques","sequence":"first","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal"},{"name":"National Institute of Agricultural and Veterinary Research, IP (INIAV), 2780-157 Oeiras, Portugal"}]},{"given":"Beatriz","family":"Pita","sequence":"additional","affiliation":[{"name":"NOVA School of Science and Technology (NOVA FCT), NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"given":"Daniel","family":"Pinto","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5605-1419","authenticated-orcid":false,"given":"Maria Teresa","family":"Barreto-Crespo","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier (ITQB), 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8879-974X","authenticated-orcid":false,"given":"Rosario","family":"Mato","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry\/Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8349-3802","authenticated-orcid":false,"given":"Teresa","family":"Semedo-Lemsaddek","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal"},{"name":"Biosystems & Integrative Sciences Institute (BioISI), Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"ref_1","unstructured":"(2025, September 28). 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