{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T09:24:02Z","timestamp":1769851442638,"version":"3.49.0"},"reference-count":56,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["UIDB\/05748\/2020"],"award-info":[{"award-number":["UIDB\/05748\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["UIDP\/05748\/2020"],"award-info":[{"award-number":["UIDP\/05748\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["SFRH\/UIDB\/151042\/2021"],"award-info":[{"award-number":["SFRH\/UIDB\/151042\/2021"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJPB"],"abstract":"<jats:p>The endomembrane system in plant cells enables the cell to manage and coordinate a variety of membranous compartments so that they and their contents arrive at the right location. The secretory pathway is an essential part of this complex network and has its gateway at the Endoplasmic Reticulum. Therefore, alterations at the ER can affect how protein trafficking takes place and how cargo leaves this organelle. With this work, we assessed how abnormalities at the Endoplasmic Reticulum would interfere with protein sorting and trafficking. We used an Arabidopsis mutant\u2014leb-2 GFP-h, presenting abnormal ER morphology, and evaluated the expression of aspartic proteinases and genes related to vacuolar transport along with the localization of a specific vacuolar sorting signal\u2014plant-specific insert (PSI). Our results show that alterations in the leb-2 GFP-h mutant did not disrupt the transport of PSI\u2013mCherry to the vacuole but influenced the expression of endogenous aspartic proteinases. Furthermore, the study of key endomembrane genes expression revealed an upregulation of the SNARE proteins AtVAMP722 and AtVAMP723. The leb-2 mutant seems not to interfere with vacuolar routes but may be implicated in secretion events.<\/jats:p>","DOI":"10.3390\/ijpb14040075","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T08:19:43Z","timestamp":1700122783000},"page":"1034-1050","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2347-7130","authenticated-orcid":false,"given":"Tatiana","family":"Cardoso","sequence":"first","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre and Inov4Agro\u2014Institute for Innovation, Training and Sustainability of Agrifood Production, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"given":"Miguel","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre and Inov4Agro\u2014Institute for Innovation, Training and Sustainability of Agrifood Production, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5910-1998","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Neves","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"given":"Sofia","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"given":"In\u00eas","family":"Moura","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"given":"Ana","family":"S\u00e9neca","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre and Inov4Agro\u2014Institute for Innovation, Training and Sustainability of Agrifood Production, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9489-9904","authenticated-orcid":false,"given":"Jos\u00e9","family":"Pissarra","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre and Inov4Agro\u2014Institute for Innovation, Training and Sustainability of Agrifood Production, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7938-1074","authenticated-orcid":false,"given":"Susana","family":"Pereira","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre and Inov4Agro\u2014Institute for Innovation, Training and Sustainability of Agrifood Production, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8050-5102","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Pereira","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"},{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre and Inov4Agro\u2014Institute for Innovation, Training and Sustainability of Agrifood Production, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1093\/pcp\/pcu049","article-title":"The Plant Endomembrane System\u2014A Complex Network Supporting Plant Development and Physiology","volume":"55","author":"Morita","year":"2014","journal-title":"Plant Cell Physiol."},{"key":"ref_2","unstructured":"Cevher-Keskin, B. (2020). Electrodialysis, IntechOpen."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.pbi.2014.09.001","article-title":"ER\u2014The Key to the Highway","volume":"22","author":"Stefano","year":"2014","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1111\/j.1600-0854.2008.00795.x","article-title":"The Endomembrane System: A Green Perspective","volume":"9","author":"Frigerio","year":"2008","journal-title":"Traffic"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1093\/jxb\/erx262","article-title":"Protein Secretion in Plants: Conventional and Unconventional Pathways and New Techniques","volume":"69","author":"Wang","year":"2018","journal-title":"J. Exp. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/jipb.12895","article-title":"The Roles of Endomembrane Trafficking in Plant Abiotic Stress Responses","volume":"62","author":"Wang","year":"2020","journal-title":"J. Integr. Plant Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1046\/j.1365-313X.1997.11061151.x","article-title":"The Plant ER: A Dynamic Organelle Composed of a Large Number of Discrete Functional Domains","volume":"11","author":"Staehelin","year":"1997","journal-title":"Plant J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.ceb.2013.02.020","article-title":"ER-PM Connections: Sites of Information Transfer and Inter-Organelle Communication","volume":"25","author":"Stefan","year":"2013","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15033","DOI":"10.1038\/celldisc.2015.33","article-title":"ER Network Homeostasis Is Critical for Plant Endosome Streaming and Endocytosis","volume":"1","author":"Stefano","year":"2015","journal-title":"Cell Discov."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3672","DOI":"10.1105\/tpc.109.068270","article-title":"GNOM-LIKE1\/ERMO1 and SEC24a\/ERMO2 Are Required for Maintenance of Endoplasmic Reticulum Morphology in Arabidopsis Thaliana","volume":"21","author":"Nakano","year":"2009","journal-title":"Plant Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1007\/BF01282989","article-title":"Endoplasmic Reticulum and Cytoplasmic Streaming: Fluorescence Microscopical Observations in Adaxial Epidermis Cells of Onion Bulb Scales","volume":"131","author":"Quader","year":"1986","journal-title":"Protoplasma"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1093\/pcp\/pcg089","article-title":"The ER Body, a Novel Endoplasmic Reticulum-Derived Structure in Arabidopsis","volume":"44","author":"Matsushima","year":"2003","journal-title":"Plant Cell Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1104\/pp.104.053876","article-title":"Diversity and Formation of Endoplasmic Reticulum-Derived Compartments in Plants. Are These Compartments Specific to Plant Cells?","volume":"136","author":"Matsushima","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sampaio, M., Neves, J., Cardoso, T., Pissarra, J., Pereira, S., and Pereira, C. (2022). Coping with Abiotic Stress in Plants\u2014An Endomembrane Trafficking Perspective. Plants, 1.","DOI":"10.3390\/plants11030338"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1105\/tpc.112.095679","article-title":"Mechanisms and Concepts Paving the Way towards a Complete Transport Cycle of Plant Vacuolar Sorting Receptors","volume":"24","author":"Gershlick","year":"2012","journal-title":"Plant Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1104\/pp.108.124552","article-title":"What Is Moving in the Secretory Pathway of Plants?","volume":"147","author":"Rojo","year":"2008","journal-title":"Plant Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1104\/pp.107.108985","article-title":"Newly Formed Vacuoles in Root Meristems of Barley and Pea Seedlings Have Characteristics of Both Protein Storage and Lytic Vacuoles","volume":"145","author":"Olbrich","year":"2007","journal-title":"Plant Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/S0092-8674(00)81256-8","article-title":"Plant Cells Contain Two Functionally Distinct Vacuolar Compartments","volume":"85","author":"Paris","year":"1996","journal-title":"Cell"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1046\/j.1432-1327.1998.2550133.x","article-title":"Identification and Proteolytic Processing of Procardosin A","volume":"255","author":"Verissimo","year":"1998","journal-title":"Eur. J. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1023\/A:1010675015318","article-title":"Molecular Cloning and Characterization of CDNA Encoding Cardosin B, an Aspartic Proteinase Accumulating Extracellularly in the Transmitting Tissue of Cynara cardunculus L","volume":"45","author":"Vieira","year":"2001","journal-title":"Plant Mol. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/s004250050183","article-title":"Cardosin A, an Abundant Aspartic Proteinase, Accumulates in Protein Storage Vacuoles in the Stigmatic Papillae of Cynara cardunculus L","volume":"203","author":"Pissarra","year":"1997","journal-title":"Planta"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s00709-008-0288-9","article-title":"Cardosins in Postembryonic Development of Cardoon: Towards an Elucidation of the Biological Function of Plant Aspartic Proteinases","volume":"232","author":"Pereira","year":"2008","journal-title":"Protoplasma"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/tpj.12274","article-title":"Cardosin A Contains Two Vacuolar Sorting Signals Using Different Vacuolar Routes in Tobacco Epidermal Cells","volume":"76","author":"Pereira","year":"2013","journal-title":"Plant J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1111\/j.1432-1033.2004.04136.x","article-title":"Structure and Function of Plant Aspartic Proteinases","volume":"271","author":"Faro","year":"2004","journal-title":"Eur. J. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1093\/jxb\/erz034","article-title":"Atypical and Nucellin-like Aspartic Proteases: Emerging Players in Plant Developmental Processes and Stress Responses","volume":"70","author":"Soares","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"38190","DOI":"10.1074\/jbc.M006093200","article-title":"The Saposin-like Domain of the Plant Aspartic Proteinase Precursor Is a Potent Inducer of Vesicle Leakage","volume":"275","author":"Egas","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.jplph.2005.08.007","article-title":"Plant-Specific Insertions in the Soybean Aspartic Proteinases, SoyAP1 and SoyAP2, Perform Different Functions of Vacuolar Targeting","volume":"163","author":"Terauchi","year":"2006","journal-title":"J. Plant Physiol."},{"key":"ref_28","first-page":"18","article-title":"In Silico Insights into Protein-Protein Interactions and Folding Dynamics of the Saposin-like Domain of Solanum Tuberosum Aspartic Protease","volume":"9","author":"Bryksa","year":"2014","journal-title":"PLoS ONE"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.phytochem.2018.02.004","article-title":"Transgenic Expression of Plant-Specific Insert of Potato Aspartic Proteases (StAP-PSI) Confers Enhanced Resistance to Botrytis Cinerea in Arabidopsis Thaliana","volume":"149","author":"Frey","year":"2018","journal-title":"Phytochemistry"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.bbamem.2013.08.004","article-title":"Possible Mechanism of Structural Transformations Induced by StAsp-PSI in Lipid Membranes","volume":"1838","author":"Daleo","year":"2014","journal-title":"Biochim. Biophys. Acta-Biomembr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/j.1365-313X.2010.04421.x","article-title":"Protein Trafficking to the Cell Wall Occurs through Mechanisms Distinguishable from Default Sorting in Tobacco","volume":"65","author":"Lenucci","year":"2011","journal-title":"Plant J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e25129","DOI":"10.4161\/psb.25129","article-title":"Unconventional Pathways of Secretory Plant Proteins from the Endoplasmic Reticulum to the Vacuole Bypassing the Golgi Complex","volume":"8","author":"Bellucci","year":"2013","journal-title":"Plant Signal. Behav."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.plaphy.2013.10.010","article-title":"Pietro Two Glycosylated Vacuolar GFPs Are New Markers for ER-to-Vacuole Sorting","volume":"73","author":"Stigliano","year":"2013","journal-title":"Plant Physiol. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1093\/jxb\/erx376","article-title":"Trafficking Routes to the Plant Vacuole: Connecting Alternative and Classical Pathways","volume":"69","author":"Barozzi","year":"2018","journal-title":"J. Exp. Bot."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Vieira, V., Peixoto, B., Costa, M., Pereira, S., Pissarra, J., and Pereira, C. (2019). N-Linked Glycosylation Modulates Golgi-Independent Vacuolar Sorting Mediated by the Plant Specific Insert. Plants, 8.","DOI":"10.3390\/plants8090312"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1093\/pcp\/pcp157","article-title":"Quantitative Analysis of ER Body Morphology in an Arabidopsis Mutant","volume":"50","author":"Nagano","year":"2009","journal-title":"Plant Cell Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/978-1-0716-0880-7_6","article-title":"Transient Transformation of A. Thaliana Seedlings by Vacuum Infiltration BT\u2014Arabidopsis Protocols","volume":"Volume 2200","author":"Salinas","year":"2021","journal-title":"Methods in Molecular Biology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1046\/j.1365-313x.1998.00343.x","article-title":"Floral Dip: A Simplified Method for Agrobacterium-Mediated Transformation of Arabidopsis Thaliana","volume":"16","author":"Clough","year":"1998","journal-title":"Plant J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1104\/pp.105.063743","article-title":"Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis","volume":"139","author":"Czechowski","year":"2005","journal-title":"Plant Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1093\/mp\/sss117","article-title":"Pietro AtSYP51\/52 Functions Diverge in the Post-Golgi Traffic and Differently Affect Vacuolar Sorting","volume":"6","author":"Bleve","year":"2013","journal-title":"Mol. Plant"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3132","DOI":"10.1105\/tpc.114.127159","article-title":"Arabidopsis SNAREs SYP61 and SYP121 Coordinate the Trafficking of Plasma Membrane Aquaporin PIP2;7 to Modulate the Cell Membrane Water Permeability","volume":"26","author":"Hachez","year":"2014","journal-title":"Plant Cell"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1365-313X.2011.04786.x","article-title":"The Trafficking Protein SYP121 of Arabidopsis Connects Programmed Stomatal Closure and K+ Channel Activity with Vegetative Growth","volume":"69","author":"Eisenach","year":"2012","journal-title":"Plant J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1111\/j.1365-313X.2010.04394.x","article-title":"Arabidopsis Qa-SNARE SYP2 Proteins Localized to Different Subcellular Regions Function Redundantly in Vacuolar Protein Sorting and Plant Development","volume":"64","author":"Shirakawa","year":"2010","journal-title":"Plant J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1105\/tpc.15.00305","article-title":"The Arabidopsis R-SNARE VAMP721 Interacts with KAT1 and KC1 K+ Channels to Moderate K+ Current at the Plasma Membrane","volume":"27","author":"Zhang","year":"2015","journal-title":"Plant Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1104\/pp.108.121566","article-title":"Secretory Pathways in Plant Immune Responses","volume":"147","author":"Kwon","year":"2008","journal-title":"Plant Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1038\/nature06545","article-title":"Co-Option of a Default Secretory Pathway for Plant Immune Responses","volume":"451","author":"Kwon","year":"2008","journal-title":"Nature"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Zhang, L., Zhang, H., Liu, P., Hao, H., Jin, J.B., and Lin, J. (2011). Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are Required for Cell Plate Formation. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0026129"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1016\/j.phytochem.2008.07.012","article-title":"Expression and Characterization of the Recombinant Aspartic Proteinase A1 from Arabidopsis Thaliana","volume":"69","author":"Yada","year":"2008","journal-title":"Phytochemistry"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Neves, J., Sampaio, M., S\u00e9neca, A., Pereira, S., Pissarra, J., and Pereira, C. (2021). Abiotic Stress Triggers the Expression of Genes Involved in Protein Storage Vacuole and Exocyst-Mediated Routes. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910644"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Neves, J., Monteiro, J., Sousa, B., Soares, C., Pereira, S., Fidalgo, F., Pissarra, J., and Pereira, C. (2022). Relevance of the Exocyst in Arabidopsis Exo70e2 Mutant for Cellular Homeostasis under Stress. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010424"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1046\/j.1365-313X.2003.01636.x","article-title":"A Novel ER-Derived Compartment, the ER Body, Selectively Accumulates a \u03b2-Glucosidase with an ER-Retention Signal in Arabidopsis","volume":"33","author":"Matsushima","year":"2003","journal-title":"Plant J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"493","DOI":"10.2174\/138920305774933268","article-title":"Aspartic Proteinase Content of the Arabidopsis Genome","volume":"6","author":"Faro","year":"2005","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4675","DOI":"10.1046\/j.1432-1033.2002.03168.x","article-title":"The Three Typical Aspartic Proteinase Genes of Arabidopsis Thaliana Are Differentially Expressed","volume":"269","author":"Chen","year":"2002","journal-title":"Eur. J. Biochem."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ito, Y., Uemura, T., and Nakano, A. (2014). Formation and Maintenance of the Golgi Apparatus in Plant Cells, Elsevier Inc.. [1st ed.].","DOI":"10.1016\/B978-0-12-800180-6.00006-2"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"631643","DOI":"10.3389\/fpls.2020.631643","article-title":"Interaction Between the SNARE SYP121 and the Plasma Membrane Aquaporin PIP2;7 Involves Different Protein Domains","volume":"11","author":"Laloux","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1104\/pp.108.121129","article-title":"SNAREs: Cogs and Coordinators in Signaling and Development","volume":"147","author":"Bassham","year":"2008","journal-title":"Plant Physiol."}],"container-title":["International Journal of Plant Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2037-0164\/14\/4\/75\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:23:53Z","timestamp":1760131433000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2037-0164\/14\/4\/75"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,16]]},"references-count":56,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["ijpb14040075"],"URL":"https:\/\/doi.org\/10.3390\/ijpb14040075","relation":{},"ISSN":["2037-0164"],"issn-type":[{"value":"2037-0164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,16]]}}}