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Current control methods are dominated by inefficient cultural practices and broad-spectrum insecticides that, in addition to having toxic effects on non-target organisms, are becoming less effective due to acquired resistance. The increasing awareness of the real impact of insecticides on health and the environment has promoted the exploration of new insecticidal compounds, addressing novel molecular targets. This study explores the efficacy of two orally delivered spider venom peptides (SVPs), J-atracotoxin-Hv1c (Hv1c) and \u00b5-theraphotoxin-Hhn2b (TRTX), to manage D. suzukii, through survival assays and the evaluation of gene expression associated with detoxification pathways. Treatment with TRTX at 111.5 \u00b5M for 48 h enhanced fly longevity compared with the control group. Gene expression analysis suggests that detoxification and stress-related mechanisms, such as expression of P450 proteins and apoptotic stimuli signaling, are triggered in D. suzukii flies in response to these treatments. Our results highlight the potential interest of SVPs to control this pest, shedding light on how to ultimately develop improved target-specific formulations.<\/jats:p>","DOI":"10.3390\/insects14060533","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T02:02:28Z","timestamp":1686189748000},"page":"533","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Towards a Sustainable Management of the Spotted-Wing Drosophila: Disclosing the Effects of Two Spider Venom Peptides on Drosophila suzukii"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6278-9727","authenticated-orcid":false,"given":"Laura","family":"Regalado","sequence":"first","affiliation":[{"name":"iB2, Biology Department, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Sciences, University of Porto, 4050-453 Porto, Portugal"}]},{"given":"Sara","family":"Sario","sequence":"additional","affiliation":[{"name":"iB2, Biology Department, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Sciences, University of Porto, 4050-453 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5212-6172","authenticated-orcid":false,"given":"Rafael J.","family":"Mendes","sequence":"additional","affiliation":[{"name":"iB2, Biology Department, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Sciences, University of Porto, 4050-453 Porto, Portugal"}]},{"given":"Javier","family":"Valle","sequence":"additional","affiliation":[{"name":"Proteomics and Protein Chemistry Unit, Department of Medicine and Life Sciences, Pompeu Fabra University, 08002 Barcelona, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4735-6242","authenticated-orcid":false,"given":"Peta J.","family":"Harvey","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, QLD 4072, Australia"}]},{"given":"C\u00e1tia","family":"Teixeira","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Faculty of Sciences, University of Porto, 4050-453 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6018-4724","authenticated-orcid":false,"given":"Paula","family":"Gomes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Faculty of Sciences, University of Porto, 4050-453 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6317-6666","authenticated-orcid":false,"given":"David","family":"Andreu","sequence":"additional","affiliation":[{"name":"Proteomics and Protein Chemistry Unit, Department of Medicine and Life Sciences, Pompeu Fabra University, 08002 Barcelona, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4129-6381","authenticated-orcid":false,"given":"Concei\u00e7\u00e3o","family":"Santos","sequence":"additional","affiliation":[{"name":"iB2, Biology Department, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Sciences, University of Porto, 4050-453 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Garcia, F.R.M. 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