{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T12:58:54Z","timestamp":1774529934489,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T00:00:00Z","timestamp":1642377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Aim: Bone disease after kidney transplant (KT) results from multiple factors, including previous bone and mineral metabolism disturbances and effects of transplant-related medications. New biomolecules have been recently associated with the development and progression of the chronic kidney disease\u2013associated bone and mineral disorder (CKD-MBD). These include sclerostin and the soluble receptor activator of nuclear factor-kB ligand (sRANKL). Methods: To better understand the role of biomarkers in post-transplant bone disease, this study was designed to prospectively evaluate and correlate results from the histomorphometric analysis of bone biopsies after KT with emerging serum biomarkers of the CKD-MBD: sclerostin, Dickkopf-related protein 1 (Dkk-1), sRANKL and osteo-protegerin (OPG). Results: Our data shows a significant increase in plasma levels of bioactive sclerostin after KT accompanied by a significant reduction in plasma levels of Dkk-1, suggesting a promotion of the inhibition of bone formation by osteoblasts through the activation of these inhibitors of the Wnt signaling pathway. In addition, we found a significant increase in plasma levels of free sRANKL after KT accompanied by a significant reduction in plasma levels of its decoy receptor OPG, suggesting an enhanced bone resorption by osteoclasts mediated by this mechanism. Conclusions: Taken together, these results suggest that the loss of bone volume observed after KT could be explain mainly by the inhibition of bone formation mediated by sclerostin accompanied by an enhanced bone resorption mediated by sRANKL.<\/jats:p>","DOI":"10.3390\/jcm11020457","type":"journal-article","created":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T08:20:42Z","timestamp":1642407642000},"page":"457","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Could Bone Biomarkers Predict Bone Turnover after Kidney Transplantation?\u2014A Proof-of-Concept Study"],"prefix":"10.3390","volume":"11","author":[{"given":"Juliana","family":"Magalh\u00e3es","sequence":"first","affiliation":[{"name":"Nephrology and Infectious Diseases Research Group, Institute for Innovation and Health Research (I3S), Institute of Biomedical Engineering (INEB), University of Porto, 4200-135 Porto, Portugal"},{"name":"Faculty of Medicine, University of Porto, 4200-250 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8388-1848","authenticated-orcid":false,"given":"Janete","family":"Quelhas-Santos","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Porto, 4200-250 Porto, Portugal"}]},{"given":"Luciano","family":"Pereira","sequence":"additional","affiliation":[{"name":"Nephrology and Infectious Diseases Research Group, Institute for Innovation and Health Research (I3S), Institute of Biomedical Engineering (INEB), University of Porto, 4200-135 Porto, Portugal"},{"name":"Nephrology Department, Faculty of Medicine, University of Porto, 4200-250 Porto, Portugal"}]},{"given":"Ricardo","family":"Neto","sequence":"additional","affiliation":[{"name":"Nephrology and Infectious Diseases Research Group, Institute for Innovation and Health Research (I3S), Institute of Biomedical Engineering (INEB), University of Porto, 4200-135 Porto, Portugal"},{"name":"Nephrology Department, Faculty of Medicine, University of Porto, 4200-250 Porto, Portugal"}]},{"given":"In\u00eas","family":"Castro-Ferreira","sequence":"additional","affiliation":[{"name":"Nephrology and Infectious Diseases Research Group, Institute for Innovation and Health Research (I3S), Institute of Biomedical Engineering (INEB), University of Porto, 4200-135 Porto, Portugal"},{"name":"Nephrology Department, Faculty of Medicine, University of Porto, 4200-250 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6813-314X","authenticated-orcid":false,"given":"Sandra","family":"Martins","sequence":"additional","affiliation":[{"name":"Centro Hospitalar de S\u00e3o Jo\u00e3o and EPI Unit, Clinical Pathology Department, Institute of Public Health, University of Porto, 4200-319 Porto, Portugal"}]},{"given":"Jo\u00e3o Miguel","family":"Fraz\u00e3o","sequence":"additional","affiliation":[{"name":"Nephrology and Infectious Diseases Research Group, Institute for Innovation and Health Research (I3S), Institute of Biomedical Engineering (INEB), University of Porto, 4200-135 Porto, Portugal"},{"name":"Nephrology Department, Faculty of Medicine, University of Porto, 4200-250 Porto, Portugal"}]},{"given":"Catarina","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Nephrology and Infectious Diseases Research Group, Institute for Innovation and Health Research (I3S), Institute of Biomedical Engineering (INEB), University of Porto, 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/nrneph.2009.192","article-title":"Bone disease after renal transplantation","volume":"6","author":"Malluche","year":"2010","journal-title":"Nat. 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