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Compos. Sci."],"abstract":"<jats:p>The development of scaffolds for tissue-engineered growth of the anterior cruciate ligament (ACL) is a promising approach to overcome the limitations of current solutions. This work proposes novel biodegradable and biocompatible scaffolds matching the mechanical characteristics of the native human ligament. Poly(L-lactic acid) (PLA) scaffolds reinforced with graphite nano-platelets (PLA+EG) as received, chemically functionalized (PLA+f-EG), or functionalized and decorated with silver nanoparticles [PLA+((f-EG)+Ag)], were fabricated by conventional braiding and using 3D-printing technology. The dimensions of both braided and 3D-printed scaffolds were finely controlled. The results showed that the scaffolds exhibited high porosity (&gt;60%), pore interconnectivity, and pore size suitable for ligament tissue ingrowth, with no relevant differences between PLA and composite scaffolds. The wet state dynamic mechanical analysis at 37 \u00b0C revealed an increase in the storage modulus of the composite constructs, compared to neat PLA scaffolds. Either braided or 3D-printed scaffolds presented storage modulus values similar to those found in soft tissues. The tailorable design of the braided structures, as well as the reproducibility, the high speed, and the simplicity of 3D-printing allowed to obtain two different scaffolds suitable for ligament tissue engineering.<\/jats:p>","DOI":"10.3390\/jcs7030104","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T01:58:22Z","timestamp":1678240702000},"page":"104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Engineering Ligament Scaffolds Based on PLA\/Graphite Nanoplatelet Composites by 3D Printing or Braiding"],"prefix":"10.3390","volume":"7","author":[{"given":"Magda","family":"Silva","sequence":"first","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s, Associate PT Government Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"},{"name":"Department of Polymer Engineering, Institute for Polymers and Composites, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"given":"Isabel","family":"Pinho","sequence":"additional","affiliation":[{"name":"Department of Polymer Engineering, Institute for Polymers and Composites, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"given":"Hugo","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Department of Polymer Engineering, Institute for Polymers and Composites, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1590-6636","authenticated-orcid":false,"given":"Ana C.","family":"Vale","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s, Associate PT Government Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3538-5804","authenticated-orcid":false,"given":"Maria C.","family":"Paiva","sequence":"additional","affiliation":[{"name":"Department of Polymer Engineering, Institute for Polymers and Composites, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8741-4091","authenticated-orcid":false,"given":"Nat\u00e1lia M.","family":"Alves","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s, Associate PT Government Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0790-5801","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Covas","sequence":"additional","affiliation":[{"name":"Department of Polymer Engineering, Institute for Polymers and Composites, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4131","DOI":"10.1016\/S0142-9612(02)00156-4","article-title":"Silk matrix for tissue engineered anterior cruciate ligaments","volume":"23","author":"Altman","year":"2002","journal-title":"Biomaterials"},{"key":"ref_2","first-page":"20","article-title":"Novel strategies in tendon and ligament tissue engineering: Advanced biomaterials and regeneration motifs","volume":"2","author":"Kuo","year":"2010","journal-title":"Sport. 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