{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T15:31:11Z","timestamp":1776871871843,"version":"3.51.2"},"reference-count":63,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:00:00Z","timestamp":1666051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"PT national funds (FCT\/MCTES, Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia and Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior)","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"PT national funds (FCT\/MCTES, Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia and Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior)","doi-asserted-by":"publisher","award":["UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDP\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Bone tissue infection is a major clinical challenge with high morbidity and a significant healthcare burden. Therapeutic approaches are usually based on systemic antibacterial therapies, despite the potential adverse effects associated with antibiotic resistance, persistent and opportunistic infections, hypersensitivity, and toxicity issues. Most recently, tissue engineering strategies, embracing local delivery systems and antibacterial biomaterials, have emerged as a promising alternative to systemic treatments. Despite the reported efficacy in managing bacterial infection, little is known regarding the outcomes of these devices on the bone healing process. Accordingly, this systematic review aims, for the first time, to characterize the efficacy of antibacterial biomaterials\/tissue engineering constructs on the healing process of the infected bone within experimental animal models and upon microtomographic characterization. Briefly, a systematic evaluation of pre-clinical studies was performed according to the PRISMA guidelines, further complemented with bias analysis and methodological quality assessments. Data reported a significant improvement in the healing of the infected bone when an antibacterial construct was implanted, compared with the control\u2014construct devoid of antibacterial activity, particularly at longer time points. Furthermore, considering the assessment of bias, most included studies revealed an inadequate reporting methodology, which may lead to an unclear or high risk of bias and directly hinder future studies.<\/jats:p>","DOI":"10.3390\/jfb13040193","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:58:51Z","timestamp":1666141131000},"page":"193","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Antimicrobial Biomaterials for the Healing of Infected Bone Tissue: A Systematic Review of Microtomographic Data on Experimental Animal Models"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5908-1437","authenticated-orcid":false,"given":"Lorena Castro","family":"Mariano","sequence":"first","affiliation":[{"name":"BoneLab\u2014Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"LAQV\/REQUIMTE, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9391-9574","authenticated-orcid":false,"given":"Maria Helena Raposo","family":"Fernandes","sequence":"additional","affiliation":[{"name":"BoneLab\u2014Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"LAQV\/REQUIMTE, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5365-2123","authenticated-orcid":false,"given":"Pedro Sousa","family":"Gomes","sequence":"additional","affiliation":[{"name":"BoneLab\u2014Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"LAQV\/REQUIMTE, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rothe, R., Hauser, S., Neuber, C., Laube, M., Schulze, S., Rammelt, S., and Pietzsch, J. (2020). Adjuvant drug-assisted bone healing: Advances and challenges in drug delivery approaches. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12050428"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Reichert, J.C., N\u00f6th, U., Berner, A., and Hutmacher, D.W. (2016). Bone. Regenerative Medicine\u2014From Protocol to Patient: 5 Regenerative Therapies II, Springer. [3rd ed.].","DOI":"10.1007\/978-3-319-28386-9_9"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.ijmm.2009.10.003","article-title":"Interaction of staphylococci with bone","volume":"300","author":"Wright","year":"2010","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3389\/fcimb.2015.00085","article-title":"Staphylococcus aureus vs. Osteoblast: Relationship and Consequences in Osteomyelitis","volume":"5","author":"Josse","year":"2015","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1002\/jor.24422","article-title":"Impact of Bacterial Infections on Osteogenesis: Evidence From In Vivo Studies","volume":"37","author":"Croes","year":"2019","journal-title":"J. Orthop. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.chom.2013.05.015","article-title":"Staphylococcus aureus osteomyelitis: Bad to the bone","volume":"13","author":"Olson","year":"2013","journal-title":"Cell Host Microbe."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.7150\/ijms.45960","article-title":"The Process of Osteoblastic Infection by Staphylococcus Aureus","volume":"17","author":"Wen","year":"2020","journal-title":"Int. J. Med. Sci."},{"key":"ref_8","first-page":"25","article-title":"Antibiotics and bacterial resistance in the 21st century","volume":"6","author":"Fair","year":"2014","journal-title":"Perspect. Med. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1080\/10717544.2021.1998246","article-title":"Recent advances in the local antibiotics delivery systems for management of osteomyelitis","volume":"28","author":"Wassif","year":"2021","journal-title":"Drug Deliv."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1517\/17425247.2011.628655","article-title":"Dual delivery of an antibiotic and a growth factor addresses both the microbiological and biological challenges of contaminated bone fractures","volume":"8","author":"Wenke","year":"2011","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"100412","DOI":"10.1016\/j.mtbio.2022.100412","article-title":"Scaffolds in the microbial resistant era: Fabrication, materials, properties and tissue engineering applications","volume":"16","author":"Aljabali","year":"2022","journal-title":"Mater. Today Bio"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/bonekey.2014.59","article-title":"Quantitative analysis of bone and soft tissue by micro-computed tomography: Applications to ex vivo and in vivo studies","volume":"3","author":"Campbell","year":"2014","journal-title":"Bonekey Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.bonr.2016.05.006","article-title":"Effect of micro-computed tomography voxel size and segmentation method on trabecular bone microstructure measures in mice","volume":"5","author":"Christiansen","year":"2016","journal-title":"Bone Rep."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Moher, D., Liberati, A., Tetzlaff, J., Altman, D.G., and PRISMA Group (2009). Preferred reporting items for systematic reviews and meta-analyses: The PRISMA statement. PLoS Med., 6.","DOI":"10.1371\/journal.pmed.1000097"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/ebm2.7","article-title":"A protocol format for the preparation, registration and publication of systematic reviews of animal intervention studies","volume":"2","author":"Hooijmans","year":"2015","journal-title":"Evid. Based Preclin. Med."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Hooijmans, C.R., Rovers, M.M., de Vries, R.B., Leenaars, M., Ritskes-Hoitinga, M., and Langendam, M.W. (2014). SYRCLE\u2019s risk of bias tool for animal studies. BMC Med. Res. Methodol., 14.","DOI":"10.1186\/1471-2288-14-43"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"332","DOI":"10.22203\/eCM.v027a24","article-title":"A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis in a contaminated in vivo bone defect model","volume":"27","author":"McLaren","year":"2014","journal-title":"Eur. Cells Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1039\/C8BM00903A","article-title":"Regenerating infected bone defects with osteocompatible microspheres possessing antibacterial activity","volume":"7","author":"Wei","year":"2018","journal-title":"Biomater. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3106","DOI":"10.1039\/C9BM01968E","article-title":"Sustainable release of vancomycin from micro-arc oxidised 3D-printed porous Ti6Al4V for treating methicillin-resistant Staphylococcus aureus bone infection and enhancing osteogenesis in a rabbit tibia osteomyelitis model","volume":"8","author":"Zhang","year":"2020","journal-title":"Biomater. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.apmt.2019.06.012","article-title":"3D printed dual-functional biomaterial with self-assembly micro-nano surface and enriched nano argentum for antibacterial and bone regeneration","volume":"17","author":"Li","year":"2019","journal-title":"Appl. Mater. Today"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1002\/jor.24507","article-title":"Efficacy of Antibiotic-Loaded Hydroxyapatite\/Collagen Composites Is Dependent on Adsorbability for Treating Staphylococcus aureus Osteomyelitis in Rats","volume":"38","author":"Egawa","year":"2020","journal-title":"J. Orthop. Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Boyle, K.K., Sosa, B., Osagie, L., Turajane, K., Bostrom, M.P.G., and Yang, X. (2019). Vancomycin-laden calcium phosphate-calcium sulfate composite allows bone formation in a rat infection model. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0222034"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s10856-019-6261-7","article-title":"Efficacy of novel nano-hydroxyapatite\/polyurethane composite scaffolds with silver phosphate particles in chronic osteomyelitis","volume":"30","author":"Zhang","year":"2019","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5855","DOI":"10.2147\/IJN.S247088","article-title":"Injectable Chitosan-Based Thermosensitive Hydrogel\/Nanoparticle-Loaded System for Local Delivery of Vancomycin in the Treatment of Osteomyelitis","volume":"15","author":"Tao","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"111807","DOI":"10.1016\/j.msec.2020.111807","article-title":"Antimicrobial hydroxyapatite and its composites for the repair of infected femoral condyle","volume":"121","author":"Tian","year":"2021","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Hasan, R., Schaner, K., Mulinti, P., and Brooks, A. (2021). A Bioglass-Based Antibiotic (Vancomycin) Releasing Bone Void Filling Putty to Treat Osteomyelitis and Aid Bone Healing. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22147736"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"025037","DOI":"10.1088\/1758-5090\/aa6ed6","article-title":"3D-printed bioceramic scaffolds with antibacterial and osteogenic activity","volume":"9","author":"Zhang","year":"2017","journal-title":"Biofabrication"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4805","DOI":"10.1039\/C9RA10275B","article-title":"Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL\/HA composite scaffolds","volume":"10","author":"Tian","year":"2020","journal-title":"RSC Adv."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5027","DOI":"10.2147\/IJN.S241859","article-title":"One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment","volume":"15","author":"Weng","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"196","DOI":"10.22203\/eCM.v027a15","article-title":"A systematic review of animal models for Staphylococcus aureus osteomyelitis","volume":"27","author":"Reizner","year":"2014","journal-title":"Eur. Cell Mater."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Calabro, L., Richards, R.G., and Moriarty, T.F. (2015). Preclinical Models of Infection in Bone and Joint Surgery. Bone and Joint Infections: From Microbiology to Diagnostics and Treatment, John Wiley & Sons.","DOI":"10.1002\/9781118581742.ch4"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"339","DOI":"10.17221\/6914-VETMED","article-title":"A comparison of the microarchitecture of lower limb long bones between some animal models and humans: A review","volume":"58","author":"Cvetkovic","year":"2013","journal-title":"Vet. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3109\/03008207.2015.1027341","article-title":"Selection of animal models for pre-clinical strategies in evaluating the fracture healing, bone graft substitutes and bone tissue regeneration and engineering","volume":"56","author":"Oryan","year":"2015","journal-title":"Connect. Tissue Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1177\/0300985815593124","article-title":"Animal Models for Evaluation of Bone Implants and Devices: Comparative Bone Structure and Common Model Uses","volume":"52","author":"Wancket","year":"2015","journal-title":"Vet. Pathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1210\/endo.139.2.5751","article-title":"Interspecies differences in bone composition, density, and quality: Potential implications for in vivo bone research","volume":"139","author":"Aerssens","year":"1998","journal-title":"Endocrinology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1002\/ame2.12083","article-title":"Animal modeling in bone research-Should we follow the White Rabbit?","volume":"2","author":"Macedo","year":"2019","journal-title":"Anim. Model Exp. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jot.2015.05.002","article-title":"Bone defect animal models for testing efficacy of bone substitute biomaterials","volume":"3","author":"Li","year":"2015","journal-title":"J. Orthop. Translat."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.22203\/eCM.v013a01","article-title":"Animal models for implant biomaterial research in bone: A review","volume":"13","author":"Pearce","year":"2007","journal-title":"Eur. Cell Mater."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1097\/00003086-198604000-00036","article-title":"The critical size defect as an experimental model for craniomandibulofacial nonunions","volume":"205","author":"Schmitz","year":"1986","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"S20","DOI":"10.1097\/BOT.0000000000000978","article-title":"Size Matters: Defining Critical in Bone Defect Size!","volume":"31","author":"Schemitsch","year":"2017","journal-title":"J. Orthop. Trauma"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"S91","DOI":"10.1016\/j.injury.2017.04.021","article-title":"Management of traumatic bone defects: Metaphyseal versus diaphyseal defects","volume":"48","author":"Blokhuis","year":"2017","journal-title":"Injury"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"172","DOI":"10.22203\/eCM.v040a11","article-title":"Animal models for studying metaphyseal bone fracture healing","volume":"40","author":"Ignatius","year":"2020","journal-title":"Eur. Cell Mater."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e00084-17","DOI":"10.1128\/CMR.00084-17","article-title":"Staphylococcal Osteomyelitis: Disease Progression, Treatment Challenges, and Future Directions","volume":"31","author":"Kavanagh","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1128\/microbiolspec.GPP3-0039-2018","article-title":"Staphylococcus aureus Secreted Toxins and Extracellular Enzymes","volume":"7","author":"Tam","year":"2019","journal-title":"Microbiol. Spectr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.msec.2017.05.017","article-title":"Scaffolds for Bone Tissue Engineering: State of the art and new perspectives","volume":"78","author":"Roseti","year":"2017","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Aoki, K., and Saito, N. (2020). Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12020095"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Dorati, R., DeTrizio, A., Modena, T., Conti, B., Benazzo, F., Gastaldi, G., and Genta, I. (2017). Biodegradable scaffolds for bone regeneration combined with drug-delivery systems in osteomyelitis therapy. Pharmaceuticals, 10.","DOI":"10.3390\/ph10040096"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1365-2842.1997.tb00311.x","article-title":"Bioactive glass particles of narrow size range for the treatment of oral bone defects: A 1-24 month experiment with several materials and particle sizes and size ranges","volume":"24","author":"Schepers","year":"1997","journal-title":"J. Oral Rehabil."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4714","DOI":"10.3390\/ijms15034714","article-title":"Current progress in bioactive ceramic scaffolds for bone repair and regeneration","volume":"15","author":"Gao","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Lu, H., Liu, Y., Guo, J., Wu, H., Wang, J., and Wu, G. (2016). Biomaterials with Antibacterial and Osteoinductive Properties to Repair Infected Bone Defects. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17030334"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1128\/AAC.03147-14","article-title":"Optimizing the Clinical Use of Vancomycin. Antimicrob","volume":"60","author":"Alvarez","year":"2016","journal-title":"Agents Chemother."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.ijid.2019.02.005","article-title":"Antibiotic penetration into bone and joints: An updated review","volume":"81","author":"Thabit","year":"2019","journal-title":"Int. J. Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Loc-Carrillo, C., Wang, C., Canden, A., Burr, M., and Agarwal, J. (2016). Local Intramedullary Delivery of Vancomycin Can Prevent the Development of Long Bone Staphylococcus aureus Infection. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0160187"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1055\/s-0029-1214162","article-title":"Local antibiotic therapy in osteomyelitis","volume":"23","author":"Gogia","year":"2009","journal-title":"Semin. Plast. Surg."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1097\/01.blo.0000175713.30506.77","article-title":"Local antibiotic delivery vehicles in the treatment of musculoskeletal infection","volume":"437","author":"Hanssen","year":"2005","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1007\/s10856-021-06590-y","article-title":"Synthesis of silver nanoparticle-decorated hydroxyapatite nanocomposite with combined bioactivity and antibacterial properties","volume":"32","author":"Bee","year":"2021","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.biomaterials.2010.09.066","article-title":"Biological actions of silver nanoparticles embedded in titanium controlled by micro-galvanic effects","volume":"32","author":"Cao","year":"2011","journal-title":"Biomaterials"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0079-6468(08)70024-9","article-title":"Antimicrobial activity and action of silver","volume":"31","author":"Russell","year":"1994","journal-title":"Prog. Med. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Fiore, M., Bruschi, A., Giannini, C., Morante, L., Rondinella, C., Filippini, M., Sambri, A., and De Paolis, M. (2022). Is Silver the New Gold? A Systematic Review of the Preclinical Evidence of Its Use in Bone Substitutes as Antiseptic. Antibiotics, 11.","DOI":"10.3390\/antibiotics11080995"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s10534-010-9381-6","article-title":"Antibacterial effect of silver nanoparticles on Staphylococcus aureus","volume":"24","author":"Li","year":"2011","journal-title":"Biometals"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"8856","DOI":"10.3390\/molecules20058856","article-title":"Silver nanoparticles as potential antibacterial agents","volume":"20","author":"Franci","year":"2015","journal-title":"Molecules"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Koopmann, A.-K., Schuster, C., Torres-Rodr\u00edguez, J., Kain, S., Pertl-Obermeyer, H., Petutschnigg, A., and H\u00fcsing, N. (2020). Tannin-Based Hybrid Materials and Their Applications: A Review. Molecules, 25.","DOI":"10.3390\/molecules25214910"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1021\/acssuschemeng.5b01407","article-title":"Tannic Acid Induced Self-Assembly of Three-Dimensional Graphene with Good Adsorption and Antibacterial Properties","volume":"4","author":"Luo","year":"2016","journal-title":"ACS Sustain. Chem. Eng."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/13\/4\/193\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:56:54Z","timestamp":1760144214000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/13\/4\/193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,18]]},"references-count":63,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["jfb13040193"],"URL":"https:\/\/doi.org\/10.3390\/jfb13040193","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,18]]}}}