{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:12:46Z","timestamp":1760148766449,"version":"build-2065373602"},"reference-count":72,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T00:00:00Z","timestamp":1686096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia and Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020","UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020","UIDP\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Calcium silicate-based cements are used in a variety of clinical conditions affecting the pulp tissue, relying on their inductive effect on tissue mineralization. This work aimed to evaluate the biological response of calcium silicate-based cements with distinct properties\u2014the fast-setting Biodentine\u2122 and TotalFill\u00ae BC RRM\u2122 Fast Putty, and the classical slow-setting ProRoot\u00ae MTA, in an ex vivo model of bone development. Briefly, eleven-day-old embryonic chick femurs were cultured for 10 days in organotypic conditions, being exposed to the set cements\u2019 eluates and, at the end of the culture period, evaluated for osteogenesis\/bone formation by combining microtomographic analysis and histological histomorphometric assessment. ProRoot\u00ae MTA and TotalFill\u00ae extracts presented similar levels of calcium ions, although significantly lower than those released from BiodentineTM. All extracts increased the osteogenesis\/tissue mineralization, assayed by microtomographic (BV\/TV) and histomorphometric (% of mineralized area; % of total collagen area, and % of mature collagen area) indexes, although displaying distinct dose-dependent patterns and quantitative values. The fast-setting cements displayed better performance than that of ProRoot\u00ae MTA, with BiodentineTM presenting the best performance, within the assayed experimental model.<\/jats:p>","DOI":"10.3390\/jfb14060314","type":"journal-article","created":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T02:02:15Z","timestamp":1686103335000},"page":"314","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ex Vivo Osteogenesis Induced by Calcium Silicate-Based Cement Extracts"],"prefix":"10.3390","volume":"14","author":[{"given":"Gabriel","family":"Kato","sequence":"first","affiliation":[{"name":"Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"LAQV\/Requimte, University of Porto, 4100-007 Porto, Portugal"}]},{"given":"Rita","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"LAQV\/Requimte, University of Porto, 4100-007 Porto, Portugal"}]},{"given":"Cl\u00e1udia","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5365-2123","authenticated-orcid":false,"given":"Pedro Sousa","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"LAQV\/Requimte, University of Porto, 4100-007 Porto, Portugal"}]},{"given":"Liliana","family":"Grenho","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"LAQV\/Requimte, University of Porto, 4100-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9391-9574","authenticated-orcid":false,"given":"Maria Helena","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"},{"name":"LAQV\/Requimte, University of Porto, 4100-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"991377","DOI":"10.3389\/fphar.2022.991377","article-title":"Trends of calcium silicate biomaterials in medical research and applications: A bibliometric analysis from 1990 to 2020","volume":"13","author":"Yin","year":"2022","journal-title":"Front. 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