{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T07:59:56Z","timestamp":1764403196115,"version":"build-2065373602"},"reference-count":59,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:00:00Z","timestamp":1667520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PNRA","award":["PNRA 16 00120","UIDB\/04326\/2020","CEECIND\/00425\/2017"],"award-info":[{"award-number":["PNRA 16 00120","UIDB\/04326\/2020","CEECIND\/00425\/2017"]}]},{"DOI":"10.13039\/501100001871","name":"Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["PNRA 16 00120","UIDB\/04326\/2020","CEECIND\/00425\/2017"],"award-info":[{"award-number":["PNRA 16 00120","UIDB\/04326\/2020","CEECIND\/00425\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FCT Scientific Employment Stimulus","award":["PNRA 16 00120","UIDB\/04326\/2020","CEECIND\/00425\/2017"],"award-info":[{"award-number":["PNRA 16 00120","UIDB\/04326\/2020","CEECIND\/00425\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMSE"],"abstract":"<jats:p>The current study focuses on the biological diversity of two strains of Antarctic diatoms (strains IMA082A and IMA088A) collected and isolated from the Ross Sea (Antarctica) during the XXXIV Italian Antarctic Expedition. Both species presented the typical morphological characters of the genus Craspedostauros: cribrate areolae, two \u201cfore-and-aft\u201d chloroplasts and a narrow \u201cstauros\u201d. This classification is congruent with the molecular phylogeny based on the concatenated 18S rDNA-rbcL-psbC alignment, which showed that these algae formed a monophyletic lineage including six taxonomically accepted species of Craspedostauros. Since the study of the evolution of this genus and of others raphe-bearing diatoms with a \u201cstauros\u201d is particularly challenging and their phylogeny is still debated, we tested alternative tree topologies to evaluate the relationships among these taxa. The metabolic fingerprinting approach was implemented for the assessment of the chemical diversity of IMA082A and IMA088A. In conclusion, combining (1) traditional morphological features used in diatoms identification, (2) phylogenetic analyses of the small subunit rDNA (18S rDNA), rbcL and psbC genes, and (3) metabolic fingerprint, we described the strains IMA082A and IMA088A as Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. as new species, respectively.<\/jats:p>","DOI":"10.3390\/jmse10111656","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T09:19:33Z","timestamp":1667553573000},"page":"1656","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov."],"prefix":"10.3390","volume":"10","author":[{"given":"Riccardo","family":"Trentin","sequence":"first","affiliation":[{"name":"Department of Biology, University of Padova, 35131 Padova, Italy"},{"name":"Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, 8005-139 Faro, Portugal"}]},{"given":"Emanuela","family":"Moschin","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, 35131 Padova, Italy"}]},{"given":"Andr\u00e9","family":"Duarte Lopes","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Department of Chemistry and Pharmacy, Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, University of the Algarve, 8005-039 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0137-3605","authenticated-orcid":false,"given":"Stefano","family":"Schiaparelli","sequence":"additional","affiliation":[{"name":"Department of Earth, Environment and Life Sciences, University of Genoa, 16132 Genova, Italy"},{"name":"Italian National Antarctic Museum (MNA, Section of Genoa), University of Genoa, 16132 Genova, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4338-7703","authenticated-orcid":false,"given":"Lu\u00edsa","family":"Cust\u00f3dio","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7850-6031","authenticated-orcid":false,"given":"Isabella","family":"Moro","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Padova, 35131 Padova, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1080\/09670262.2012.741714","article-title":"Metabolic fingerprinting reveals differences between northern and southern strains of the cryptic diatom Chaetoceros socialis","volume":"47","author":"Huseby","year":"2012","journal-title":"Eur. 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