{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:42:36Z","timestamp":1762522956104,"version":"build-2065373602"},"reference-count":126,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portugal 2020 program","award":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]},{"name":"MARE\u2014Marine and Environmental Sciences Centre","award":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]},{"name":"Associate Laboratory ARNET","award":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]},{"name":"FCT","award":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]},{"name":"FCT","award":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"],"award-info":[{"award-number":["MAR20-01-77P3-FEAMP-000006","UIDB\/04292\/2020","UIDP\/04292\/2020","LA\/P\/0069\/2020","2020.07688.BD","UIDB\/04423\/2020","UIDP\/04423\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMSE"],"abstract":"<jats:p>Managing fishery resources is crucial to ensure the marine environment continues to provide diverse goods and services. To overcome difficulties of classical methods used for fish stock management, molecular tools have shown potential to address this issue assessing both targeted and non-targeted species. This study aims to evaluate the spatiotemporal diversity of fish using 12S rRNA gene eDNA metabarcoding sequencing in the Berlengas archipelago and compare two seawater eDNA sampling sources: samples collected by fishermen during their activities and those collected by our research team. The results indicated that autumn presented the highest diversity and that the area around Berlenga Island was the richest area, increasing biodiversity across the region. Fisher-collected samples were generally less diverse than those by the research team but detected species typical of deeper and open-ocean habitats, validating this sampling method. Our study also highlighted eDNA\u2019s role in monitoring fish species by detecting unexpected species for the region, such as Atlantic salmon (Salmo salar) and Atlantic cod (Gadus morhua), while cautioning against false positives like orange clownfish (Amphiprion percula) and blue tilapia (Oreochromis aureus). Future optimisation of our eDNA sampling methodology could better refine marine ecosystem dynamics around the UNESCO Biosphere Reserve of the Berlengas Archipelago, Portugal.<\/jats:p>","DOI":"10.3390\/jmse13010060","type":"journal-article","created":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T13:37:51Z","timestamp":1735738671000},"page":"60","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unveiling the UNESCO Biosphere Reserve of the Berlengas Archipelago in Portugal as a Hotspot of Fish Species Using eDNA Metabarcoding and the Collaboration of Fishing Crews"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7995-7304","authenticated-orcid":false,"given":"Marco","family":"Sim\u00f5es","sequence":"first","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre\/ARNET\u2014Aquatic Research Network Associate Laboratory, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"},{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, 4450-208 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8558-5786","authenticated-orcid":false,"given":"C\u00e1tia","family":"Costa","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre\/ARNET\u2014Aquatic Research Network Associate Laboratory, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"},{"name":"Department of Life Sciences, Centre for Functional Ecology, Associate Laboratory TERRA, Faculty of Sciences and Technology, University of Coimbra, 3000-456 Coimbra, Portugal"},{"name":"CESAM\u2014Centre for Environmental and Marine Studies, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Maria","family":"da Luz Calado","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre\/ARNET\u2014Aquatic Research Network Associate Laboratory, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2162-3787","authenticated-orcid":false,"given":"Nuno","family":"Vasco-Rodrigues","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre\/ARNET\u2014Aquatic Research Network Associate Laboratory, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0883-0610","authenticated-orcid":false,"given":"Maria Jorge","family":"Campos","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre\/ARNET\u2014Aquatic Research Network Associate Laboratory, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"}]},{"given":"S\u00e9rgio Miguel","family":"Leandro","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre\/ARNET\u2014Aquatic Research Network Associate Laboratory, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-630 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1328-1732","authenticated-orcid":false,"given":"Agostinho","family":"Antunes","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, 4450-208 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s10750-005-1115-3","article-title":"Biogeographic patterns of intertidal macroinvertebrates and their association with macroalgae distribution along the Portuguese coast","volume":"555","author":"Pereira","year":"2006","journal-title":"Hydrobiologia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2592","DOI":"10.1111\/j.1365-2486.2007.01451.x","article-title":"Do distributional shifts of northern and southern species of algae match the warming pattern?","volume":"13","author":"Lima","year":"2007","journal-title":"Glob. 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