{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T12:38:22Z","timestamp":1768826302183,"version":"3.49.0"},"reference-count":83,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005285","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s","doi-asserted-by":"publisher","award":["201810267000022\/INCT-Estrat\u00e9gias de Intera\u00e7\u00e3o Pat\u00f3geno-Hospedeiro - FAPEG"],"award-info":[{"award-number":["201810267000022\/INCT-Estrat\u00e9gias de Intera\u00e7\u00e3o Pat\u00f3geno-Hospedeiro - FAPEG"]}],"id":[{"id":"10.13039\/501100005285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000013"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000013"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Paracoccidioides spp. are thermally dimorphic fungi that cause paracoccidioidomycosis and can affect both immunocompetent and immunocompromised individuals. The infection can lead to moderate or severe illness and death. Paracoccidioides spp. undergo micronutrients deprivation within the host, including iron. To overcome such cellular stress, this genus of fungi responds in multiple ways, such as the utilization of hemoglobin. A glycosylphosphatidylinositol (GPI)-anchored fungal receptor, Rbt5, has the primary role of acquiring the essential nutrient iron from hemoglobin. Conversely, it is not clear if additional proteins participate in the process of using hemoglobin by the fungus. Therefore, in order to investigate changes in the proteomic level of P. lutzii cell wall, we deprived the fungus of iron and then treated those cells with hemoglobin. Deprived iron cells were used as control. Next, we performed cell wall fractionation and the obtained proteins were submitted to nanoUPLC-MSE. Protein expression levels of the cell wall F1 fraction of cells exposed to hemoglobin were compared with the protein expression of the cell wall F1 fraction of iron-deprived cells. Our results showed that P. lutzii exposure to hemoglobin increased the level of adhesins expression by the fungus, according to the proteomic data. We confirmed that the exposure of the fungus to hemoglobin increased its ability to adhere to macrophages by flow cytometry. In addition, we found that HSP30 of P. lutzii is a novel hemoglobin-binding protein and a possible heme oxygenase. In order to investigate the importance of HSP30 in the Paracoccidioides genus, we developed a Paracoccidioides brasiliensis knockdown strain of HSP30 via Agrobacterium tumefaciens-mediated transformation and demonstrated that silencing this gene decreases the ability of P. brasiliensis to use hemoglobin as a nutrient source. Additional studies are needed to establish HSP30 as a virulence factor, which can support the development of new therapeutic and\/or diagnostic approaches.<\/jats:p>","DOI":"10.3390\/jof7010021","type":"journal-article","created":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T22:35:48Z","timestamp":1609540548000},"page":"21","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Interacting with Hemoglobin: Paracoccidioides spp. Recruits hsp30 on Its Cell Surface for Enhanced Ability to Use This Iron Source"],"prefix":"10.3390","volume":"7","author":[{"given":"Aparecido Ferreira","family":"de Souza","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, ICB II, Campus II, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74000-000, Brazil"}]},{"given":"Mariana Vieira","family":"Tomazett","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, ICB II, Campus II, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74000-000, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5350-7313","authenticated-orcid":false,"given":"Kleber Santiago","family":"Freitas e Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, ICB II, Campus II, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74000-000, Brazil"}]},{"given":"Juliana Santana","family":"de Curcio","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, ICB II, Campus II, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74000-000, Brazil"}]},{"given":"Christie Ataides","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, ICB II, Campus II, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74000-000, Brazil"}]},{"given":"Lilian Cristiane","family":"Baeza","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias M\u00e9dicas e Farmac\u00eauticas, Universidade Estadual do Oeste do Paran\u00e1, Cascavel 85819-110, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1086-4588","authenticated-orcid":false,"given":"Juliano Domiraci","family":"Paccez","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, ICB II, Campus II, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74000-000, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3037-3663","authenticated-orcid":false,"given":"Relber Aguiar","family":"Gon\u00e7ales","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4700-000 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4800-000 Guimar\u00e3es, Portugal"}]},{"given":"Fernando","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4700-000 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4800-000 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5482-8036","authenticated-orcid":false,"given":"Maristela","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, ICB II, Campus II, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74000-000, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5378-9840","authenticated-orcid":false,"given":"C\u00e9lia Maria","family":"de Almeida Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, ICB II, Campus II, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74000-000, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1111\/j.1462-5822.2010.01529.x","article-title":"The struggle for iron\u2014A metal at the host-pathogen interface","volume":"12","author":"Nairz","year":"2010","journal-title":"Cell. 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