{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T10:23:56Z","timestamp":1768040636069,"version":"3.49.0"},"reference-count":147,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,22]],"date-time":"2022-05-22T00:00:00Z","timestamp":1653177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CESAM by FCT\/MCTES","award":["UIDP\/50017\/2020+UIDB\/50017\/2020+ LA\/P\/0094\/2020"],"award-info":[{"award-number":["UIDP\/50017\/2020+UIDB\/50017\/2020+ LA\/P\/0094\/2020"]}]},{"name":"CESAM by FCT\/MCTES","award":["PTDC\/BIA-BIC\/3488\/2012"],"award-info":[{"award-number":["PTDC\/BIA-BIC\/3488\/2012"]}]},{"name":"CESAM by FCT\/MCTES","award":["PD\/BD\/113605\/2015"],"award-info":[{"award-number":["PD\/BD\/113605\/2015"]}]},{"DOI":"10.13039\/501100001871","name":"the research project Generosi","doi-asserted-by":"publisher","award":["UIDP\/50017\/2020+UIDB\/50017\/2020+ LA\/P\/0094\/2020"],"award-info":[{"award-number":["UIDP\/50017\/2020+UIDB\/50017\/2020+ LA\/P\/0094\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"the research project Generosi","doi-asserted-by":"publisher","award":["PTDC\/BIA-BIC\/3488\/2012"],"award-info":[{"award-number":["PTDC\/BIA-BIC\/3488\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"the research project Generosi","doi-asserted-by":"publisher","award":["PD\/BD\/113605\/2015"],"award-info":[{"award-number":["PD\/BD\/113605\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"the grants from FCT to SC","doi-asserted-by":"publisher","award":["UIDP\/50017\/2020+UIDB\/50017\/2020+ LA\/P\/0094\/2020"],"award-info":[{"award-number":["UIDP\/50017\/2020+UIDB\/50017\/2020+ LA\/P\/0094\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"the grants from FCT to SC","doi-asserted-by":"publisher","award":["PTDC\/BIA-BIC\/3488\/2012"],"award-info":[{"award-number":["PTDC\/BIA-BIC\/3488\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"the grants from FCT to SC","doi-asserted-by":"publisher","award":["PD\/BD\/113605\/2015"],"award-info":[{"award-number":["PD\/BD\/113605\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Amphibians constitute the class of vertebrates with the highest proportion of threatened species, with infectious diseases being considered among the greatest causes for their worldwide decline. Aquatic oomycetes, known as \u201cwater molds\u201d, are fungus-like microorganisms that are ubiquitous in freshwater ecosystems and are capable of causing disease in a broad range of amphibian hosts. Various species of Achlya sp., Leptolegnia sp., Aphanomyces sp., and mainly, Saprolegnia sp., are responsible for mass die-offs in the early developmental stages of a wide range of amphibian populations through a disease known as saprolegniosis, aka, molding or a \u201cSaprolegnia-like infection\u201d. In this context, the main objective of the present review was to bring together updated information about saprolegniosis in amphibians to integrate existing knowledge, identify current knowledge gaps, and suggest future directions within the saprolegniosis\u2013amphibian research field. Based on the available literature and data, an integrated and critical interpretation of the results is discussed. Furthermore, the occurrence of saprolegniosis in natural and laboratory contexts and the factors that influence both pathogen incidence and host susceptibility are also addressed. The focus of this work was the species Saprolegnia sp., due to its ecological importance on amphibian population dynamics and due to the fact that this is the most reported genera to be associated with saprolegniosis in amphibians. In addition, integrated emerging therapies, and their potential application to treat saprolegniosis in amphibians, were evaluated, and future actions are suggested.<\/jats:p>","DOI":"10.3390\/jof8050537","type":"journal-article","created":{"date-parts":[[2022,5,22]],"date-time":"2022-05-22T07:13:57Z","timestamp":1653203637000},"page":"537","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Saprolegniosis in Amphibians: An Integrated Overview of a Fluffy Killer Disease"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3504-1972","authenticated-orcid":false,"given":"Sara","family":"Costa","sequence":"first","affiliation":[{"name":"CESAM & Department of Biology Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9325-0329","authenticated-orcid":false,"given":"Isabel","family":"Lopes","sequence":"additional","affiliation":[{"name":"CESAM & Department of Biology Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1093\/czoolo\/59.3.427","article-title":"Climate change and infectious diseases of wildlife: Altered interactions between pathogens, vectors and hosts","volume":"59","author":"Gallana","year":"2013","journal-title":"Curr. Zool."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1461-0248.2006.00890.x","article-title":"Predator disease out-break modulates top-down, bottom-up and climatic effects on herbivore population dynamics","volume":"9","author":"Wilmers","year":"2006","journal-title":"Ecol. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1111\/j.1523-1739.2006.00524.x","article-title":"Evidence for the role of infectious disease in species extinction and endangerment","volume":"20","author":"Smith","year":"2006","journal-title":"Conserv. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3165","DOI":"10.1073\/pnas.0506889103","article-title":"Emerging infectious disease and the loss of biodiversity in a Neotropical amphibian community","volume":"103","author":"Lips","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"735","DOI":"10.3201\/eid0506.990601","article-title":"Emerging Infectious Diseases and Amphibian Population Declines","volume":"5","author":"Daszak","year":"1999","journal-title":"Emerg. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1046\/j.1472-4642.2003.00016.x","article-title":"Infectious disease and amphibian population declines","volume":"9","author":"Daszak","year":"2003","journal-title":"Divers. Distrib."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1126\/science.aav0379","article-title":"Amphibian fungal panzootic causes catastrophic and ongoing loss of biodiversity","volume":"363","author":"Scheele","year":"2019","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-69486-x","article-title":"Batrachochytrium salamandrivorans (Bsal) not detected in an intensive survey of wild North American amphibians","volume":"10","author":"Waddle","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15325","DOI":"10.1073\/pnas.1307356110","article-title":"Batrachochytrium salamandrivorans sp. nov. causes lethal chytridiomycosis in amphibians","volume":"110","author":"Martel","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","unstructured":"(2020, January 01). OIE OIE-Listed Diseases, Infections and Infestations in Force in 2020. Available online: https:\/\/www.oie.int\/animal-health-in-the-world\/oie-listed-diseases-2020\/."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.aaf.2018.12.003","article-title":"Molecular identification of oomycete species affecting aquaculture in Bangladesh","volume":"4","author":"Sarowar","year":"2019","journal-title":"Aquac. Fish."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1111\/j.1365-2427.2010.02546.x","article-title":"The ecology and emergence of diseases in fresh waters","volume":"56","author":"Johnson","year":"2011","journal-title":"Freshw. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11049","DOI":"10.1073\/pnas.92.24.11049","article-title":"Synergism between UV-B radiation and a pathogen magnifies amphibian embryo mortality in nature","volume":"92","author":"Kiesecker","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1046\/j.1523-1739.2001.0150041064.x","article-title":"Transfer of a Pathogen from Fish to Amphibians","volume":"15","author":"Kiesecker","year":"2001","journal-title":"Conserv. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.funbio.2014.05.005","article-title":"Saprolegnia strains isolated from river insects and amphipods are broad spectrum pathogens","volume":"118","author":"Sarowar","year":"2014","journal-title":"Fungal Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1890\/08-0079.1","article-title":"The ecology of climate change and infectious diseases","volume":"90","author":"Lafferty","year":"2009","journal-title":"Ecology"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Grogan, L.F., Berger, L., Rose, K., Grillo, V., Cashins, S.D., and Skerratt, L.F. (2014). Surveillance for Emerging Biodiversity Diseases of Wildlife. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1004015"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.pbi.2010.04.001","article-title":"Oomycete\u2013plant coevolution: Recent advances and future prospects","volume":"13","author":"Thines","year":"2010","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1053\/saep.2002.123980","article-title":"An overview of amphibian skin disease","volume":"11","author":"Pessier","year":"2002","journal-title":"Semin. Avian Exot. Pet Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/ilar.48.3.235","article-title":"Diseases of amphibians","volume":"48","author":"Densmore","year":"2007","journal-title":"ILAR J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"363","DOI":"10.2307\/3544324","article-title":"Habitat Selection of Amphibians during Their Aquatic Phase","volume":"33","author":"Strijbosch","year":"1979","journal-title":"Oikos"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/BF01946687","article-title":"Effects of water acidification on the distribution pattern and the reproductive success of amphibians","volume":"42","author":"Leuven","year":"1986","journal-title":"Experientia"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"475","DOI":"10.2307\/4919","article-title":"Reproductive Success of Natterjack Toads Bufo calamita in Two Contrasting Habitats","volume":"57","author":"Banks","year":"1988","journal-title":"J. Anim. Ecol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1080\/04416651.2010.495674","article-title":"An enigmatic mortality event in the only population of the critically endangered cameroonian frog Xenopus longipes","volume":"59","author":"Blackburn","year":"2010","journal-title":"Afr. J. Herpetol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"43","DOI":"10.5818\/1529-9651-21.2.43","article-title":"Saprolegniasis in a Chinese Giant Salamander (Andrias davidianus)","volume":"21","author":"Chowdry","year":"2012","journal-title":"J. Herpetol. Med. Surg."},{"key":"ref_26","first-page":"25","article-title":"Comparison of methods for controlling Saprolegnia-like infection in the egg sacks of Asiatic salamanders (Hynobius)","volume":"140","author":"Michaels","year":"2018","journal-title":"Herpetol. Bull."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1139\/cjz-2019-0221","article-title":"Water mould exposure induces enzymatic antioxidant defences in embryos of the Two-colored Oval Frog (Elachistocleis bicolor) (Anura: Microhylidae)","volume":"98","author":"Ghirardi","year":"2020","journal-title":"Can. J. Zool."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.fbr.2018.12.001","article-title":"Comprehensive, classical and molecular characterization methods of Saprolegnia (Oomycota; Stramnipila), an important fungal pathogen of fish","volume":"33","author":"Magray","year":"2019","journal-title":"Fungal Biol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.fbr.2013.05.001","article-title":"The impact of the water moulds Saprolegnia diclina and Saprolegnia parasitica on natural ecosystems and the aquaculture industry","volume":"27","author":"McLaggan","year":"2013","journal-title":"Fungal Biol. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.fgb.2007.02.010","article-title":"Re-evaluation of the enigmatic species complex Saprolegnia diclina-Saprolegnia parasitica based on morphological, physiological and molecular data","volume":"44","author":"Cerenius","year":"2007","journal-title":"Fungal Genet. Biol."},{"key":"ref_31","first-page":"116","article-title":"DNA barcoding as a means to identify organisms associated with amphibian eggs","volume":"3","author":"Johnson","year":"2008","journal-title":"Herpetol. Conserv. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Sandoval-Sierra, J.V., and Di\u00e9guez-Uribeondo, J. (2015). A Comprehensive Protocol for Improving the Description of Saprolegniales (Oomycota): Two Practical Examples (Saprolegnia aenigmatica sp. nov. and Saprolegnia racemosa sp. nov.). PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0132999"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/bs.adgen.2020.03.001","article-title":"Recent advances in oomycete genomics","volume":"105","author":"McGowan","year":"2020","journal-title":"Advances in Genetics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/s11557-018-1381-x","article-title":"New phylogenetic insights into Saprolegniales (Oomycota, Straminipila) based upon studies of specimens isolated from Brazil and Argentina","volume":"17","author":"Rocha","year":"2018","journal-title":"Mycol. Prog."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1080\/15572536.2007.11832567","article-title":"Species boundaries within Saprolegnia (Saprolegniales, Oomycota) based on morphological and DNA sequence data","volume":"99","author":"Hulvey","year":"2007","journal-title":"Mycologia"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.funbio.2013.10.005","article-title":"Species identification in the genus Saprolegnia (Oomycetes): Defining DNA-based molecular operational taxonomic units","volume":"118","year":"2014","journal-title":"Fungal Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_38","first-page":"e01173","article-title":"Climate\u2019s cascading effects on disease, predation, and hatching success in Anaxyrus canorus, the threatened Yosemite toad","volume":"23","author":"Sadinski","year":"2020","journal-title":"Glob. Ecol. Conserv."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"234","DOI":"10.11144\/javeriana.SC16-3.fros","article-title":"Primer registro de Saprolegnia sp. en una poblaci\u00f3n de anfibios en Colombia","volume":"16","year":"2011","journal-title":"Univ. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1163\/156853898X00151","article-title":"Aquatic fungi growing on the spawn of certain amphibians","volume":"19","author":"Czeczuga","year":"1998","journal-title":"Amphibia-Reptilia"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2570","DOI":"10.1890\/0012-9658(2006)87[2570:AEAPDA]2.0.CO;2","article-title":"Amphibian embryo and parental defenses and a larval predator reduce egg mortality from water mold","volume":"87","author":"Touchon","year":"2006","journal-title":"Ecology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1139\/z06-058","article-title":"Hatching plasticity in two temperate anurans: Responses to a pathogen and predation cues","volume":"84","author":"Touchon","year":"2006","journal-title":"Can. J. Zool."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0006-3207(94)90616-5","article-title":"Pathogenic fungus contributes to amphibian losses in the pacific northwest","volume":"67","author":"Blaustein","year":"1994","journal-title":"Biol. Conserv."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1046\/j.1523-1739.1997.95509.x","article-title":"Influences of Egg Laying Behavior on Pathogenic Infection of Amphibian Eggs","volume":"11","author":"Kiesecker","year":"1997","journal-title":"Conserv. Biol."},{"key":"ref_45","first-page":"161","article-title":"Susceptibility of newly-metamorphosed frogs to a pathogenic water mould (Saprolegnia sp.)","volume":"17","author":"Romansic","year":"2007","journal-title":"Herpetol. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3354\/dao02456","article-title":"Genetic comparison of water molds from embryos of amphibians Rana cascadae, Bufo boreas and Pseudacris regilla","volume":"99","author":"Ault","year":"2012","journal-title":"Dis. Aquat. Organ."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1163\/156853803322440745","article-title":"Susceptibility of frog (Rana temporaria) and toad (Bufo bufo) eggs to invasion by Saprolegnia","volume":"24","author":"Robinson","year":"2003","journal-title":"Amphibia-Reptilia"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1574-6968.2007.01002.x","article-title":"Saprolegnia diclina: Another species responsible for the emergent disease \u2018Saprolegnia infections\u2019 in amphibians","volume":"279","author":"Lizana","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1007\/s00442-010-1889-5","article-title":"Differences in susceptibility to Saprolegnia infections among embryonic stages of two anuran species","volume":"165","author":"Lizana","year":"2011","journal-title":"Oecologia"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3354\/dao03434","article-title":"High mortality in Bufo gargarizans eggs associated with an undescribed Saprolegnia ferax strain in the Republic of Korea","volume":"137","author":"Groffen","year":"2019","journal-title":"Dis. Aquat. Organ."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"330","DOI":"10.2307\/1566126","article-title":"Mucocutaneous fungal disease in tadpoles of Bufo marinus in Australia","volume":"35","author":"Berger","year":"2001","journal-title":"J. Herpetol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1071\/WR9940621","article-title":"Population ecology of the Australian frog Crinia signifera: Egg-laying patterns and egg mortality","volume":"21","author":"Williamson","year":"1994","journal-title":"Wildl. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1080\/23766808.2016.1151133","article-title":"Rainbow trout (Oncorhynchus mykiss) threaten Andean amphibians","volume":"2","author":"Bosch","year":"2016","journal-title":"Neotrop. Biodivers."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2442","DOI":"10.1890\/0012-9658(1999)080[2442:PRCBLA]2.0.CO;2","article-title":"Pathogen reverses competition between larval amphibians","volume":"80","author":"Kiesecker","year":"1999","journal-title":"Ecology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3354\/dao068235","article-title":"Effects of nitrate and the pathogenic water mold Saprolegnia on survival of amphibian larvae","volume":"68","author":"Romansic","year":"2006","journal-title":"Dis. Aquat. Organ."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"79","DOI":"10.2307\/3669450","article-title":"Saprolegnia on tadpoles again in Oklahoma","volume":"7","author":"Bragg","year":"1962","journal-title":"Southwest. Nat."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3354\/dao02512","article-title":"Multihost saprobes are facultative pathogens of bullfrog Lithobates catesbeianus eggs","volume":"101","author":"Ruthig","year":"2012","journal-title":"Dis. Aquat. Organ."},{"key":"ref_58","first-page":"216","article-title":"Evaluation of stress responses to water molds in embryos of Physalaemus albonotatus","volume":"13","author":"Ghirardi","year":"2018","journal-title":"Herpetol. Conserv. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1111\/aec.12014","article-title":"Early breeding protects anuran eggs from Saprolegnia infection","volume":"38","author":"Perotti","year":"2013","journal-title":"Austral Ecol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3354\/dao02042","article-title":"Water molds of the genera Saprolegnia and Leptolegnia are pathogenic to the North American frogs Rana catesbeiana and Pseudacris crucifer, respectively","volume":"84","author":"Ruthig","year":"2009","journal-title":"Dis. Aquat. Organ."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1080\/15572536.2008.11832474","article-title":"Saprolegniaceae identified on amphibian eggs throughout the Pacific Northwest, USA, by internal transcribed spacer sequences and phylogenetic analysis","volume":"100","author":"Petrisko","year":"2008","journal-title":"Mycologia"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"187","DOI":"10.3354\/dao02007","article-title":"Effects of the pathogenic water mold Saprolegnia ferax on survival of amphibian larvae","volume":"83","author":"Romansic","year":"2009","journal-title":"Dis. Aquat. Organ."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s10682-007-9168-6","article-title":"Intraspecific variation in resistance of frog eggs to fungal infection","volume":"22","author":"Sagvik","year":"2008","journal-title":"Evol. Ecol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Sagvik, J., Uller, T., and Olsson, M. (2008). A genetic component of resistance to fungal infection in frog embryos. Proc. R. Soc. Lond. B Biol. Sci., 275.","DOI":"10.1098\/rspb.2008.0078"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s00442-009-1280-6","article-title":"Pre-hatching exposure to water mold reduces size at metamorphosis in the moor frog","volume":"160","author":"Uller","year":"2009","journal-title":"Oecologia"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.envres.2008.09.001","article-title":"Effect of road deicing salt on the susceptibility of amphibian embryos to infection by water molds","volume":"109","author":"Karraker","year":"2009","journal-title":"Environ. Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"10","DOI":"10.4489\/MYCO.2008.36.1.010","article-title":"Detection of infectious fungal diseases of frogs inhabiting in Korea","volume":"36","author":"Kim","year":"2008","journal-title":"Mycobiology"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1007\/s00442-008-1026-x","article-title":"The influence of temperature and spatial distribution on the susceptibility of southern leopard frog eggs to disease","volume":"156","author":"Ruthig","year":"2008","journal-title":"Oecologia"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"346","DOI":"10.2307\/2404134","article-title":"A field study of fertilization and embryonic development in the common frog (Rana temporaria) with particular reference to acidity and temperature","volume":"28","author":"Beattie","year":"1991","journal-title":"J. Appl. Ecol."},{"key":"ref_70","first-page":"257","article-title":"Spatial variation in species composition of Saprolegnia, a parasitic oomycete of amphibian eggs, in Scotland","volume":"25","author":"Muir","year":"2015","journal-title":"Herpetol. J."},{"key":"ref_71","first-page":"34","article-title":"Parasitism of spadefoot tadpoles by Saprolegnia","volume":"14","author":"Bragg","year":"1957","journal-title":"Herpetologica"},{"key":"ref_72","first-page":"713","article-title":"Cutaneous acariasis in the African clawed frog (Xenopus laevis)","volume":"54","author":"Ford","year":"2004","journal-title":"Comp. Med."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1139\/z87-446","article-title":"Aggression and exploitation as mechanisms of competition in larval salamanders","volume":"65","author":"Walls","year":"1987","journal-title":"Can. J. Zool."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/oik.01598","article-title":"Population of origin and environment interact to determine oomycete infections in spotted salamander populations","volume":"124","author":"Urban","year":"2015","journal-title":"Oikos"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1163\/1568539X-00003126","article-title":"Singly laid mole salamander (Ambystoma talpoideum) eggs resist mortality from water mold infection","volume":"151","author":"Croshaw","year":"2014","journal-title":"Behaviour"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1007\/s002449900200","article-title":"The Effects of Used Motor Oil, Silt, and the Water Mold Saprolegnia parasitica on the Growth and Survival of Mole Salamanders (Genus Ambystoma)","volume":"32","author":"Lefcort","year":"1997","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/jzo.12615","article-title":"Preoviposition paternal care in a fully aquatic giant salamander: Nest cleaning by a den master","volume":"307","author":"Terry","year":"2019","journal-title":"J. Zool."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1046\/j.1365-2435.1999.00336.x","article-title":"Implications of pathogenic fungi for life-history evolution in amphibians","volume":"13","author":"Green","year":"1999","journal-title":"Funct. Ecol."},{"key":"ref_79","unstructured":"(2022, February 18). AmphibiaWeb. Available online: https:\/\/amphibiaweb.org\/index.html."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Jiang, R.H.Y., de Bruijn, I., Haas, B.J., Belmonte, R., L\u00f6bach, L., Christie, J., van den Ackerveken, G., Bottin, A., Bulone, V., and D\u00edaz-Moreno, S.M. (2013). Distinctive Expansion of Potential Virulence Genes in the Genome of the Oomycete Fish Pathogen Saprolegnia parasitica. PLoS Genet., 9.","DOI":"10.1371\/journal.pgen.1003272"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.3389\/fmicb.2017.02658","article-title":"Quantitative proteomic analysis of four developmental stages of Saprolegnia parasitica","volume":"8","author":"Srivastava","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_82","unstructured":"(2020, January 11). QGIS.org QGIS. Available online: https:\/\/qgis.org\/en\/site\/."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Cramp, R.L., and Franklin, C.E. (2018). Exploring the link between ultraviolet B radiation and immune function in amphibians: Implications for emerging infectious diseases. Conserv. Physiol., 6.","DOI":"10.1093\/conphys\/coy035"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1890\/0012-9658(2002)083[2951:OCONWP]2.0.CO;2","article-title":"Optical characteristics of natural waters protect amphibians from UV-B in the U.S. Pacific Northwest","volume":"83","author":"Palen","year":"2004","journal-title":"Ecology"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Savory, F., Leonard, G., and Richards, T.A. (2015). The Role of Horizontal Gene Transfer in the Evolution of the Oomycetes. PLOS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004805"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"2662","DOI":"10.1002\/lno.11242","article-title":"The contrasting roles of aquatic fungi and oomycetes in the degradation and transformation of polymeric organic matter","volume":"64","author":"Masigol","year":"2019","journal-title":"Limnol. Oceanogr."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/j.fgb.2004.06.001","article-title":"Biomechanical evidence for convergent evolution of the invasive growth process among fungi and oomycete water molds","volume":"41","author":"Money","year":"2004","journal-title":"Fungal Genet. Biol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/jfd.12368","article-title":"Saprolegnia diclina IIIA and S. parasitica employ different infection strategies when colonizing eggs of Atlantic salmon, Salmo salar L.","volume":"39","author":"Songe","year":"2016","journal-title":"J. Fish Dis."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3354\/dao013155","article-title":"Winter saprolegniosis in channel catfish","volume":"13","author":"Bly","year":"1992","journal-title":"Dis. Aquat. Organ."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Rezinciuc, S., Sandoval-Sierra, J.V., Ruiz-Le\u00f3n, Y., Van West, P., and Di\u00e9guez-Uribeondo, J. (2018). Specialized attachment structure of the fish pathogenic oomycete Saprolegnia parasitica. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0190361"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/S0953-7562(09)81060-5","article-title":"Repeated zoospore emergence in Saprolegnia parasitica","volume":"98","author":"Cerenius","year":"1994","journal-title":"Mycol. Res."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0007-1536(84)80004-2","article-title":"Influence of environmental factors on zoospores of Saprolegnia diclina","volume":"82","author":"Smith","year":"1984","journal-title":"Trans. Br. Mycol. Soc."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/S10267-002-0089-5","article-title":"Chemotactic and chemokinetic activities of Saprolegnia parasitica toward different metabolites and fish tissue extracts","volume":"44","author":"Hatai","year":"2003","journal-title":"Mycoscience"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1577\/1548-8667(1993)005<0240:COZOTF>2.3.CO;2","article-title":"Chemotaxis of zoospores of two fish-egg-pathogenic strains of saprolegnia diclina (oomycotina: Saprolegniaceae) toward salmonid egg chorion extracts and selected amino acids and sugars","volume":"5","author":"Rand","year":"1993","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"e00408-17","DOI":"10.1128\/mSphere.00408-17","article-title":"Genomic, Network, and Phylogenetic Analysis of the Oomycete Effector Arsenal","volume":"2","author":"McGowan","year":"2017","journal-title":"mSphere"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.funeco.2014.04.001","article-title":"Multiple zoosporic parasites pose a significant threat to amphibian populations","volume":"11","author":"Gleason","year":"2014","journal-title":"Fungal Ecol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.dci.2016.07.002","article-title":"Amphibian immunity\u2013stress, disease, and climate change","volume":"66","year":"2017","journal-title":"Dev. Comp. Immunol."},{"key":"ref_98","first-page":"123","article-title":"Influence of pH, Temperature and Sodium Chloride Concentration on Growth Rate of Saprolegnia sp.","volume":"30","author":"Koeypudsa","year":"2005","journal-title":"J. Sci. Res. Chula. Univ."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1577\/A03-058.1","article-title":"Changes in Water Hardness Influence Colonization of Saprolegnia diclina","volume":"66","author":"Barnes","year":"2004","journal-title":"N. Am. J. Aquac."},{"key":"ref_100","unstructured":"Wright, K.M., and Whitaker, B.R. (2001). Amphibian Medicine and Captive Husbandry, Krieger Publishing Company."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3390\/d2020281","article-title":"Direct and Indirect Effects of Climate Change on Amphibian Populations","volume":"2","author":"Blaustein","year":"2010","journal-title":"Diversity"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1111\/gcb.12720","article-title":"Warmer winters reduce frog fecundity and shift breeding phenology, which consequently alters larval development and metamorphic timing","volume":"21","author":"Benard","year":"2015","journal-title":"Glob. Chang. Biol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1046\/j.1461-0248.2003.00480.x","article-title":"How should environmental stress affect the population dynamics of disease?","volume":"6","author":"Lafferty","year":"2003","journal-title":"Ecol. Lett."},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Becker, M.H., Walke, J.B., Cikanek, S., Savage, A.E., Mattheus, N., Santiago, C.N., Minbiole, K.P.C., Harris, R.N., Belden, L.K., and Gratwicke, B. (2015). Composition of symbiotic bacteria predicts survival in Panamanian golden frogs infected with a lethal fungus. Proc. R. Soc. B Biol. Sci., 282.","DOI":"10.1098\/rspb.2014.2881"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3354\/dao02391","article-title":"Bacterial skin flora variation and in vitro inhibitory activity against Saprolegnia parasitica in brown and rainbow trout","volume":"96","year":"2011","journal-title":"Dis. Aquat. Organ."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Antwis, R.E., Haworth, R.L., Engelmoer, D.J.P., Ogilvy, V., Fidgett, A.L., and Preziosi, R.F. (2014). Ex situ Diet Influences the Bacterial Community Associated with the Skin of Red-Eyed Tree Frogs (Agalychnis callidryas). PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0085563"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.scitotenv.2016.07.230","article-title":"Diversity of cutaneous microbiome of Pelophylax perezi populations inhabiting different environments","volume":"572","author":"Costa","year":"2016","journal-title":"Sci. Total Environ."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"4518","DOI":"10.1128\/IAI.02196-14","article-title":"Role of pathogen-derived cell wall carbohydrates and prostaglandin E2 in immune response and suppression of fish immunity by the oomycete Saprolegnia parasitica","volume":"82","author":"Belmonte","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1053\/j.jepm.2008.10.003","article-title":"Edematous Frogs, Urinary Tract Disease, and Disorders of Fluid Balance in Amphibians","volume":"18","author":"Pessier","year":"2009","journal-title":"J. Exot. Pet Med."},{"key":"ref_110","unstructured":"National Research Council (US) Subcommittee on Amphibian Standards (1974). Amphibians\u2014Guidelines for the Breeding, Care and Management of Laboratory Animals, National Academies Press (US)."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S1094-9194(17)30103-2","article-title":"Amphibian Therapeutics","volume":"3","author":"Walker","year":"2000","journal-title":"Vet. Clin. N. Am. Exot. Anim. Pract."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-55314-4","article-title":"Effects of disease, antibiotic treatment and recovery trajectory on the microbiome of farmed seabass (Dicentrarchus labrax)","volume":"9","author":"Rosado","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1053\/j.jepm.2008.10.005","article-title":"Wound Management in Amphibians: Etiology and Treatment of Cutaneous Lesions","volume":"18","author":"Poll","year":"2009","journal-title":"J. Exot. Pet Med."},{"key":"ref_114","unstructured":"Mader, D.R. (1996). Amphibian Husbandry and Medicine, W.B. Saunders. Reptile Me."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1093\/ilar.48.3.297","article-title":"Compendium of Drugs and Compounds Used in Amphibians","volume":"48","author":"Smith","year":"2007","journal-title":"ILAR J."},{"key":"ref_116","unstructured":"Kirk, R., and Bonagura, J. (1992). Amphibian Medicine, W.B. Saunders. Kirk\u2019s Cur."},{"key":"ref_117","unstructured":"Ewert, J., Cooper, J.E., Langton, T., Matz, G., Reilly, K., and Schwantje, H. (2004, January 22\u201324). Background information On the species-specific proposals for amphibians. Proceedings of the 8th Meeting of the Working Party, Strasbourg, France."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1016\/S0195-5616(93)50155-9","article-title":"Amphibians","volume":"23","author":"Raphael","year":"1993","journal-title":"Vet. Clin. N. Am.-Small Anim. Pract."},{"key":"ref_119","unstructured":"Ali, S.E., Evensen, \u00d8., and Skaar, I. (2015). Recent advances in the mitigation of Saprolegnia infections in freshwater fish and their eggs. Battle Against Microb. Pathog. Basic Sci. Technol. Adv. Educ. Prog., 692\u2013697."},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Ali, S.E., Thoen, E., Evensen, O., and Skaar, I. (2014). Boric Acid inhibits germination and colonization of Saprolegnia spores in vitro and in vivo. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0091878"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"6154","DOI":"10.1128\/AEM.01195-14","article-title":"Clotrimazole as a Potent Agent for Treating the Oomycete Fish Pathogen Saprolegnia parasitica through Inhibition of Sterol 14\u03b1-Demethylase (CYP51)","volume":"80","author":"Warrilow","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"e5706","DOI":"10.7717\/peerj.5706","article-title":"The efficacy of Virkon-S for the control of saprolegniasis in common carp, Cyprinus carpio L.","volume":"2018","author":"Rahman","year":"2018","journal-title":"PeerJ"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1080\/13880200390501839","article-title":"Effect of selected essential oils and pure compounds on Saprolegnia parasitica","volume":"41","author":"Tampieri","year":"2003","journal-title":"Pharm. Biol."},{"key":"ref_124","first-page":"1","article-title":"Antifungal and toxicity effects of new combined essential oils on Oncorhynchus mykiss in comparison with malachite green","volume":"4","author":"Mousavi","year":"2012","journal-title":"Iran. J. Vet. Sci. Technol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1016\/j.sjbs.2020.04.008","article-title":"Anti-saprolegnia potency of some plant extracts against Saprolegnia diclina, the causative agent of saprolengiasis","volume":"27","author":"Mostafa","year":"2020","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Miljanovi\u0107, A., Grbin, D., Pavi\u0107, D., Dent, M., Jerkovi\u0107, I., Marijanovi\u0107, Z., and Bielen, A. (2021). Essential oils of sage, rosemary, and bay laurel inhibit the life stages of oomycete pathogens important in aquaculture. Plants, 10.","DOI":"10.3390\/plants10081676"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"8033","DOI":"10.3390\/molecules20058033","article-title":"Chemical characterization and anti-oomycete activity of Laureliopsis philippianna essential oils against Saprolegnia parasitica and S. australis","volume":"20","author":"Madrid","year":"2015","journal-title":"Molecules"},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"83","DOI":"10.3389\/fvets.2020.00083","article-title":"Comparative Therapeutic Effects of Natural Compounds Against Saprolegnia spp. (Oomycota) and Amyloodinium ocellatum (Dinophyceae)","volume":"7","author":"Tedesco","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_129","first-page":"3239","article-title":"Antifungal activity and chemical composition of Iranian medicinal herbs against fish pathogenic fungus, Saprolegnia parasitica","volume":"19","author":"Adel","year":"2020","journal-title":"Iran. J. Fish. Sci."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.biocel.2011.12.002","article-title":"Frog skin epithelium: Electrolyte transport and chytridiomycosis","volume":"44","author":"Campbell","year":"2012","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"113","DOI":"10.3354\/dao01838","article-title":"Electrolyte depletion and osmotic imbalance in amphibians with chytridiomycosis","volume":"77","author":"Voyles","year":"2007","journal-title":"Dis. Aquat. Organ."},{"key":"ref_132","unstructured":"Wright, K. (2006, January 7\u201311). Important clinical aspects of amphibian physiology. Proceedings of the the North American Veterinary Conference-Exotics\u2014Reptiles and Amphibians, Orlando, FL, USA."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1093\/toxsci\/kfw138","article-title":"Boric Acid Is Reproductively Toxic to Adult Xenopus laevis, but Not Endocrine Active","volume":"154","author":"Fort","year":"2016","journal-title":"Toxicol. Sci."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/s002449900423","article-title":"Effects of Boron and Nitrate on Hatching Success of Amphibian Eggs","volume":"35","author":"Laposata","year":"1998","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1080\/02772248.2011.634643","article-title":"Effects of clotrimazole and amiodarone on early development of amphibian (Xenopus tropicalis)","volume":"94","author":"Shi","year":"2012","journal-title":"Toxicol. Environ. Chem."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/17386357.2007.9647309","article-title":"Effect of endocrine disruptors on the oocyte maturation and ovulation in amphibians, Rana dybowskii","volume":"11","author":"Choi","year":"2007","journal-title":"Integr. Biosci."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.aquatox.2008.10.005","article-title":"Clotrimazole exposure modulates aromatase activity in gonads and brain during gonadal differentiation in Xenopus tropicalis frogs","volume":"91","author":"Gyllenhammar","year":"2009","journal-title":"Aquat. Toxicol."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1163\/156853809788795245","article-title":"Assessing whether disinfectants against the fungus Batrachochytrium dendrobatidis have negative effects on tadpoles and zooplankton","volume":"30","author":"Schmidt","year":"2009","journal-title":"Amphib. Reptil."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1163\/15685381-00002837","article-title":"Effects of the disinfectant Virkon S on early life-stages of the moor frog (Rana arvalis)","volume":"33","author":"Hangartner","year":"2012","journal-title":"Amphib. Reptil."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1051\/alr\/2018001","article-title":"Current knowledge on use of essential oils as alternative treatment against fish parasites","volume":"31","year":"2018","journal-title":"Aquat. Living Resour."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1177\/0192623311399785","article-title":"Evaluation of the toxicity of eugenol at anesthetic doses in African clawed frogs (Xenopus laevis)","volume":"39","author":"Goulet","year":"2011","journal-title":"Toxicol. Pathol."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1111\/j.1467-2995.2012.00753.x","article-title":"The effect of three anaesthetic protocols on the stress response in cane toads (Rhinella marina)","volume":"39","author":"Sernia","year":"2012","journal-title":"Vet. Anaesth. Analg."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1053\/j.jepm.2008.11.006","article-title":"Anesthetic Considerations for Amphibians","volume":"18","author":"Mitchell","year":"2009","journal-title":"J. Exot. Pet Med."},{"key":"ref_144","doi-asserted-by":"crossref","unstructured":"Salbego, J., and Maia, J.L. (2017). dos S.; Toni, C.; Rodrigues, A.S.S.; Sousa, E.M.O.; Silva, L.V.F. da; Mour\u00e3o, R.H.V.; Barata, L.E.S.; Heinzmann, B.M.; Baldisserotto, B. Anesthesia and sedation of map treefrog (Hypsiboas geographicus) tadpoles with essential oils. Ci\u00eancia Rural, 47.","DOI":"10.1590\/0103-8478cr20160909"},{"key":"ref_145","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-G\u00f3mez, O., Byrne, A.Q., Gunderson, A.R., Jenkinson, T.S., Noss, C.F., Rothstein, A.P., Womack, M.C., and Rosenblum, E.B. (2020). Invasive vegetation affects amphibian skin microbiota and body condition. PeerJ, 2020.","DOI":"10.7717\/peerj.8549"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1111\/j.1095-8649.2010.02566.x","article-title":"Current knowledge on non-native freshwater fish introductions","volume":"76","author":"Gozlan","year":"2010","journal-title":"J. Fish Biol."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.aquaculture.2014.09.005","article-title":"Saprolegnia species affecting the salmonid aquaculture in Chile and their associations with fish developmental stage","volume":"434","author":"Zaror","year":"2014","journal-title":"Aquaculture"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/5\/537\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:16:33Z","timestamp":1760138193000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/5\/537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,22]]},"references-count":147,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["jof8050537"],"URL":"https:\/\/doi.org\/10.3390\/jof8050537","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,22]]}}}