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We recently provided a Saccharomyces cerevisiae strain able to strongly overproduce succinic acid from glycerol and CO2 in which the Dct-02 transporter from Aspergillus niger, assumed to be an anion channel, was used to export succinic acid from the cells. In a different study, we reported a new group of succinic acid transporters from the AceTr family, which were also described as anion channels. Here, we expressed these transporters in a succinic acid overproducing strain and compared their impact on extracellular succinic acid accumulation with that of the Dct-02 transporter. The results show that the tested transporters of the AceTr family hinder succinic acid accumulation in the extracellular medium at low pH, which is in strong contrast to Dct-02. Data suggests that the AceTr transporters prefer monovalent succinate, whereas Dct-02 prefers divalent succinate anions. In addition, the results provided deeper insights into the characteristics of Dct-02, showing its ability to act as a succinic acid importer (thus being bidirectional) and verifying its capability of exporting malate.<\/jats:p>","DOI":"10.3390\/jof8080822","type":"journal-article","created":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T22:51:46Z","timestamp":1659912706000},"page":"822","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Dicarboxylate Transporters from the AceTr Family and Dct-02 Oppositely Affect Succinic Acid Production in S. cerevisiae"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8203-4575","authenticated-orcid":false,"given":"Toni","family":"Renduli\u0107","sequence":"first","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"School of Science, Jacobs University Bremen gGmbH, Campus Ring 1, 28759 Bremen, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0394-3819","authenticated-orcid":false,"given":"Frederico","family":"Mendon\u00e7a Bahia","sequence":"additional","affiliation":[{"name":"School of Science, Jacobs University Bremen gGmbH, Campus Ring 1, 28759 Bremen, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8431-1567","authenticated-orcid":false,"given":"Isabel","family":"Soares-Silva","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7947-3000","authenticated-orcid":false,"given":"Elke","family":"Nevoigt","sequence":"additional","affiliation":[{"name":"School of Science, Jacobs University Bremen gGmbH, Campus Ring 1, 28759 Bremen, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0143-9758","authenticated-orcid":false,"given":"Margarida","family":"Casal","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"fnaa118","DOI":"10.1093\/femsle\/fnaa118","article-title":"Membrane transporters in the bioproduction of organic acids: State of the art and future perspectives for industrial applications","volume":"367","author":"Ribas","year":"2020","journal-title":"FEMS Microbiol. 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