{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T01:00:08Z","timestamp":1775696408034,"version":"3.50.1"},"reference-count":84,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T00:00:00Z","timestamp":1666569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project Phytophthora Research Centre","award":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000453"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000453"]}]},{"name":"Czech Ministry for Education, Youth and Sports","award":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000453"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000453"]}]},{"name":"European Regional Development Fund","award":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000453"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000453"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>We explored the virome of the \u201cPhytophthora palustris complex\u201d, a group of aquatic specialists geographically limited to Southeast and East Asia, the native origin of many destructive invasive forest Phytophthora spp. Based on high-throughput sequencing (RNAseq) of 112 isolates of \u201cP. palustris\u201d collected from rivers, mangroves, and ponds, and natural forests in subtropical and tropical areas in Indonesia, Taiwan, and Japan, 52 putative viruses were identified, which, to varying degrees, were phylogenetically related to the families Botybirnaviridae, Narnaviridae, Tombusviridae, and Totiviridae, and the order Bunyavirales. The prevalence of all viruses in their hosts was investigated and confirmed by RT-PCR. The rich virus composition, high abundance, and distribution discovered in our study indicate that viruses are naturally infecting taxa from the \u201cP. palustris complex\u201d in their natural niche, and that they are predominant members of the host cellular environment. Certain Indonesian localities are the viruses\u2019 hotspots and particular \u201cP. palustris\u201d isolates show complex multiviral infections. This study defines the first bi-segmented bunya-like virus together with the first tombus-like and botybirna-like viruses in the genus Phytophthora and provides insights into the spread and evolution of RNA viruses in the natural populations of an oomycete species.<\/jats:p>","DOI":"10.3390\/jof8111118","type":"journal-article","created":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T11:53:55Z","timestamp":1666612435000},"page":"1118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Natural Populations from the Phytophthora palustris Complex Show a High Diversity and Abundance of ssRNA and dsRNA Viruses"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6613-5405","authenticated-orcid":false,"given":"Leticia","family":"Botella","sequence":"first","affiliation":[{"name":"Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zem\u011bd\u011blsk\u00e1 3, 613 00 Brno, Czech Republic"},{"name":"Department of Genetics and Agrobiotechnology, Faculty of Agriculture and Technology, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Na S\u00e1dk\u00e1ch 1780, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2219-8647","authenticated-orcid":false,"given":"Mar\u00edlia Horta","family":"Jung","sequence":"additional","affiliation":[{"name":"Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zem\u011bd\u011blsk\u00e1 3, 613 00 Brno, Czech Republic"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4081-6392","authenticated-orcid":false,"given":"Michael","family":"Rost","sequence":"additional","affiliation":[{"name":"Department of Genetics and Agrobiotechnology, Faculty of Agriculture and Technology, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Na S\u00e1dk\u00e1ch 1780, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2034-0718","authenticated-orcid":false,"given":"Thomas","family":"Jung","sequence":"additional","affiliation":[{"name":"Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zem\u011bd\u011blsk\u00e1 3, 613 00 Brno, Czech Republic"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1111\/j.1365-3059.2008.01886.x","article-title":"The biosecurity threat to the UK and global environment from international trade in plants","volume":"57","author":"Brasier","year":"2008","journal-title":"Plant Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/j.1469-8137.2012.04364.x","article-title":"Biogeographical patterns and determinants of invasion by forest pathogens in Europe","volume":"197","author":"Santini","year":"2013","journal-title":"New Phytol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.envsci.2013.09.011","article-title":"International plant trade associated risks: Laissez-faire or novel solutions","volume":"37","author":"Hantula","year":"2014","journal-title":"Environ. 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