{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T11:06:56Z","timestamp":1770462416252,"version":"3.49.0"},"reference-count":98,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Centre for Functional Ecology\u2014Science for People &amp; the Planet (CFE) and co-funded by Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"Centre for Functional Ecology\u2014Science for People &amp; the Planet (CFE) and co-funded by Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior (MCTES) from Fundo Social Europeu (FSE)","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior (MCTES) from Fundo Social Europeu (FSE)","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"FCT\/MCTES through national funds (PIDDAC)","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"FCT\/MCTES through national funds (PIDDAC)","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"Recovery and Resilience Plan (PRR)","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"Recovery and Resilience Plan (PRR)","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"the NextGeneration EU European","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"the NextGeneration EU European","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>In this study, we explored the biodiversity and abundance of culturable fungi in four samples associated with different biodeterioration outlines collected from the Lemos Pantheon, a limestone-built artwork in Portugal. We compared the results from prolonged standard freezing with those previously obtained from fresh samples to analyze differences in the obtained community and assess the effectiveness of the standard freezing incubation protocol in uncovering a different segment of culturable fungal diversity. Our results showed a slight decrease in culturable diversity, but over 70% of the obtained isolates were not present in the previously studied fresh samples. We also identified a high number of potential new species with this procedure. Moreover, the use of a wide variety of selective culture media positively influenced the diversity of the cultivable fungi obtained in this study. These findings highlight the importance of developing new protocols under varying conditions to accurately characterize the culturable fraction in a given sample. The identification and study of these communities and their possible contribution to the biodeterioration process is crucial knowledge for formulating effective conservation and restoration plans to prevent further damage to valuable cultural heritage assets.<\/jats:p>","DOI":"10.3390\/jof9040501","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T06:54:19Z","timestamp":1682060059000},"page":"501","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Exploring Differences in Culturable Fungal Diversity Using Standard Freezing Incubation\u2014A Case Study in the Limestones of Lemos Pantheon (Portugal)"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-0213","authenticated-orcid":false,"given":"Diana S.","family":"Paiva","sequence":"first","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8587-9469","authenticated-orcid":false,"given":"Lu\u00eds","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4385-4498","authenticated-orcid":false,"given":"Em\u00edlia","family":"Pereira","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7845-6046","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Trov\u00e3o","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"FitoLab\u2014Laboratory for Phytopathology, Instituto Pedro Nunes, Rua Pedro Nunes, 3030-199 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1748-6364","authenticated-orcid":false,"given":"Nuno","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9301-3585","authenticated-orcid":false,"given":"Igor","family":"Tiago","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1748-6345","authenticated-orcid":false,"given":"Ant\u00f3nio","family":"Portugal","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"FitoLab\u2014Laboratory for Phytopathology, Instituto Pedro Nunes, Rua Pedro Nunes, 3030-199 Coimbra, Portugal"},{"name":"TERRA\u2014Associate Laboratory for Sustainable Land Use and Ecosystem Services, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"780","DOI":"10.3389\/fmicb.2019.00780","article-title":"Living at the Extremes: Extremophiles and the Limits of Life in a Planetary Context","volume":"10","author":"Merino","year":"2019","journal-title":"Front. 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