{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:23:13Z","timestamp":1770880993381,"version":"3.50.1"},"reference-count":101,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T00:00:00Z","timestamp":1694304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Centre for Functional Ecology\u2014Science for People &amp; the Planet (CFE)","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"Centre for Functional Ecology\u2014Science for People &amp; the Planet (CFE)","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"Centre for Functional Ecology\u2014Science for People &amp; the Planet (CFE)","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]},{"name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior (MCTES)","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior (MCTES)","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior (MCTES)","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]},{"name":"Fundo social Europeu (FSE)","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"Fundo social Europeu (FSE)","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"Fundo social Europeu (FSE)","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]},{"name":"national funds (PIDDAC)","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"national funds (PIDDAC)","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"national funds (PIDDAC)","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]},{"name":"PRR\u2014Recovery and Resilience Plan","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"PRR\u2014Recovery and Resilience Plan","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"PRR\u2014Recovery and Resilience Plan","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]},{"name":"NextGeneration EU European Funds","award":["UI\/BD\/150843\/2021"],"award-info":[{"award-number":["UI\/BD\/150843\/2021"]}]},{"name":"NextGeneration EU European Funds","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"NextGeneration EU European Funds","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>With an impressive ability to survive in harsh environments, black fungi are an ecological group of melanized fungi that are widely recognized as a major contributor to the biodeterioration of stone cultural heritage materials. As part of the ongoing efforts to study the fungal diversity thriving in a deteriorated limestone funerary art piece at the Lemos Pantheon, a national monument located in \u00c1gueda, Portugal, two isolates of an unknown microcolonial black fungus were retrieved. These isolates were thoroughly studied through a comprehensive analysis based on a multi-locus phylogeny of a combined dataset of ITS rDNA, LSU, and rpb2, along with morphological, physiological, and ecological characteristics. Based on the data obtained from this integrative analysis, we propose a new genus, Saxispiralis gen. nov., and a new species, Saxispiralis lemnorum sp. nov., in the recently described Aeminiaceae family (order Mycosphaerellales). Prior to this discovery, this family only had one known genus and species, Aeminium\u00a0ludgeri, also isolated from deteriorated limestone. Additionally, considering the isolation source of the fungus and to better understand its potential contribution to the overall stone monument biodeterioration, its in vitro biodeteriorative potential was also evaluated. This work represents a significant contribution to the understanding of the fungal diversity involved in the biodeterioration of limestone heritage.<\/jats:p>","DOI":"10.3390\/jof9090916","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T10:48:28Z","timestamp":1694429308000},"page":"916","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Expanding the Microcolonial Black Fungi Aeminiaceae Family: Saxispiralis lemnorum gen. et sp. nov. (Mycosphaerellales), Isolated from Deteriorated Limestone in the Lemos Pantheon, Portugal"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-0213","authenticated-orcid":false,"given":"Diana S.","family":"Paiva","sequence":"first","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7845-6046","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Trov\u00e3o","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8587-9469","authenticated-orcid":false,"given":"Lu\u00eds","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1748-6364","authenticated-orcid":false,"given":"Nuno","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"given":"Igor","family":"Tiago","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1748-6345","authenticated-orcid":false,"given":"Ant\u00f3nio","family":"Portugal","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology (CFE)\u2014Science for People & the Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"FitoLab\u2014Laboratory for Phytopathology, Instituto Pedro Nunes (IPN), Rua Pedro Nunes, 3030-199 Coimbra, Portugal"},{"name":"TERRA\u2014Associate Laboratory for Sustainable Land Use and Ecosystem Services, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/2192-1709-2-1","article-title":"Early life on land and the first terrestrial ecosystems","volume":"2","year":"2013","journal-title":"Ecol. 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